Dissecting the Spatial Heterogeneity of Single Circulating Tumor Cells in Hepatocellular Carcinoma

The dismal outcome of hepatocellular carcinoma (HCC) is closely associated with intra- and extrahepatic metastases, which have been proposed to be initiated by circulating tumor cells (CTCs). However, the plasticity of gene expression and immune evasion mechanisms of CTCs during systemic circulation are not well defined. Here, we determined intra- and inter-vascular transcriptional heterogeneity within the circulatory system using single-cell RNA-sequencing of 113 CTCs isolated from four key vascular sites along their dissemination route in ten HCC patients.

Type: Other
Archiver: European Genome-Phenome Archive (EGA)

1 Dataset 3 Publications

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Dataset ID	Description	Technology	Samples
EGAD00001007642	We determined intra- and inter-vascular transcriptional heterogeneity within the circulatory system using single-cell RNA-sequencing of 113 CTCs isolated from four key vascular sites along their dissemination route in ten HCC patients. The dataset consists of 146 paired-end fastq files from 113 sigle CTCs, HuH-7 cell line, Hep3B cellline, white blood cell, tumor and normal bulk tissue.	Illumina HiSeq 4000	146

Publications	Citations
Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinoma. Sun YF, Wu L, Liu SP, Jiang MM, Hu B, Zhou KQ, Guo W, Xu Y, Zhong Y, Zhou XR, Zhang ZF, Liu G, Liu S, Shi YH, Ji Y, Du M, Li NN, Li GB, Zhao ZK, Huang XY, Xu LQ, Yu QC, Peng DH, Qiu SJ, Sun HC, Dean M, Wang XD, Chung WY, Dennison AR, Zhou J, Hou Y, Fan J, Yang XR. Nat Commun 12: 2021 4091	75
Single-Cell Transcriptomics of Liver Cancer: Hype or Insights? Zhang QY, Ho DW, Tsui YM, Ng IO. Cell Mol Gastroenterol Hepatol 14: 2022 513-525	7
Analyzing sex imbalance in EGA and dbGaP biological databases: Recommendations for better practices. Ruiz-Serra V, Buslón N, Philippe OR, Saby D, Morales M, Pontes C, Andirkó AM, Holliday GL, Jené A, Moldes M, Rambla J, Valencia A, Rementeria MJ, Cortés A, Cirillo D. iScience 27: 2024 110831	0