Single cell RNA sequencing of relapsed/refractory multiple myeloma

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Dataset ID	Description	Technology	Samples
EGAD00001006903	This dataset includes 87 scRNA-seq samples of bone marrow aspirates of 20 relapsed/refractory patients generated with the 3´(v2) kit of the 10x Chromium platform. Bone marrow cells have been sorted using CD138 +/- fractions using magnetic beads for plasma cell enrichment and processed independently. For 14/20 patients multiple treatment timepoints are available that includes samples before treatment and at relapse during treatment.	Illumina HiSeq 4000	87
EGAD00001008150	Four PAIRED WGS samples, tumor and control, were sequenced on a HiSeq X Ten and the library preparation kit used was Illumina TruSeq Nano DNA. The tumor was multiple myeloma from bone marrow.	HiSeq X Ten	4

Publications	Citations
Subclone-specific microenvironmental impact and drug response in refractory multiple myeloma revealed by single-cell transcriptomics. Tirier SM, Mallm JP, Steiger S, Poos AM, Awwad MHS, Giesen N, Casiraghi N, Susak H, Bauer K, Baumann A, John L, Seckinger A, Hose D, Müller-Tidow C, Goldschmidt H, Stegle O, Hundemer M, Weinhold N, Raab MS, Rippe K. Nat Commun 12: 2021 6960	99
Resolving therapy resistance mechanisms in multiple myeloma by multiomics subclone analysis. Poos AM, Prokoph N, Przybilla MJ, Mallm JP, Steiger S, Seufert I, John L, Tirier SM, Bauer K, Baumann A, Rohleder J, Munawar U, Rasche L, Kortüm KM, Giesen N, Reichert P, Huhn S, Müller-Tidow C, Goldschmidt H, Stegle O, Raab MS, Rippe K, Weinhold N. Blood 142: 2023 1633-1646	40
The biological and clinical impact of deletions before and after large chromosomal gains in multiple myeloma. Cirrincione AM, Poos AM, Ziccheddu B, Kaddoura M, Bärtsch MA, Maclachlan K, Chojnacka M, Diamond B, John L, Reichert P, Huhn S, Blaney P, Gagler D, Rippe K, Zhang Y, Dogan A, Lesokhin AM, Davies F, Goldschmidt H, Fenk R, Weisel KC, Mai EK, Korde N, Morgan GJ, Usmani S, Landgren O, Raab MS, Weinhold N, Maura F. Blood 144: 2024 771-783	9
Enhancer Extrachromosomal Circular DNA ANKRD28 Elicits Drug Resistance via POU2F2-Mediated Transcriptional Network in Multiple Myeloma. Chen B, Liu J, Zhang Y, Shi C, Zhu D, Zhang G, Xiao F, Zhong L, Zhang M, Ng LG, Huang H, Lu T, Hou J. Adv Sci (Weinh) 12: 2025 e2415695	2
Biallelic antigen escape is a mechanism of resistance to anti-CD38 antibodies in multiple myeloma. Diamond B, Baughn L, Poorebrahim M, Poos AM, Lee H, Kaddoura M, Wiedmeier-Nutor JE, Durante M, Otteson G, Jevremovic D, Tang H, Fröhling S, Baertsch MA, Papadimitriou M, Ziccheddu B, Jelinek T, Lemoine C, Rak A, Green DJ, Landgren O, Neri P, Bergsagel L, Braggio E, Kumar S, Raab MS, Fonseca R, Bahlis N, Weinhold N, Maura F. Blood 146: 2025 1575-1585	3