Copied to clipboard!

Single-cell RNA-sequencing reveals that glioblastoma recapitulates a normal neurodevetlopmental hierarchy

We discovered a conserved neural tri- lineage cancer hierarchy centered around glial progenitor-like cells. We also found that this progenitor population contains the majority of the cancer’s cycling cells, and, using RNA velocity, is often the originator of the other cell types. Finally, we show that this hierarchal map can be used to identify therapeutic targets specific to progenitor cancer stem cells. Our analyses show that normal brain development reconciles glioblastoma development, suggests a possible origin for glioblastoma hierarchy, and helps to identify cancer stem cell-specific targets.

Type: Other
Archive: European Genome-phenome Archive (EGA)

1 Dataset 15 Publications

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD00001006206	10xGenomics single-cell RNA sequencing of glioblastoma patient tumor	Illumina HiSeq 4000	25

Publications	Citations
Single-cell RNA-seq reveals that glioblastoma recapitulates a normal neurodevelopmental hierarchy. Couturier CP, Ayyadhury S, Le PU, Nadaf J, Monlong J, Riva G, Allache R, Baig S, Yan X, Bourgey M, Lee C, Wang YCD, Wee Yong V, Guiot MC, Najafabadi H, Misic B, Antel J, Bourque G, Ragoussis J, Petrecca K. Nat Commun 11: 2020 3406	492
Analysis of Cellular Heterogeneity in Immune Microenvironment of Primary Central Nervous System Lymphoma by Single-Cell Sequencing. Wei B, Liu Z, Fan Y, Wang S, Dong C, Rao W, Yang F, Cheng G, Zhang J. Front Oncol 11: 2021 683007	19
Profiling Glioblastoma Cases with an Expression of DCX, OLIG2 and NES. Odrzywolski A, Jarosz B, Kiełbus M, Telejko I, Ziemianek D, Knaga S, Rola R. Int J Mol Sci 22: 2021 13217	4
Glioblastoma scRNA-seq shows treatment-induced, immune-dependent increase in mesenchymal cancer cells and structural variants in distal neural stem cells. Couturier CP, Nadaf J, Li Z, Baig S, Riva G, Le P, Kloosterman DJ, Monlong J, Nkili Meyong A, Allache R, Degenhard T, Al-Rashid M, Guiot MC, Bourque G, Ragoussis J, Akkari L, Quintana FJ, Petrecca K. Neuro Oncol 24: 2022 1494-1508	47
A single-cell atlas of glioblastoma evolution under therapy reveals cell-intrinsic and cell-extrinsic therapeutic targets. Wang L, Jung J, Babikir H, Shamardani K, Jain S, Feng X, Gupta N, Rosi S, Chang S, Raleigh D, Solomon D, Phillips JJ, Diaz AA. Nat Cancer 3: 2022 1534-1552	312
Glioblastoma heterogeneity at single cell resolution. Eisenbarth D, Wang YA. Oncogene 42: 2023 2155-2165	148
Kunitz-type protease inhibitor TFPI2 remodels stemness and immunosuppressive tumor microenvironment in glioblastoma. Pang L, Dunterman M, Guo S, Khan F, Liu Y, Taefi E, Bahrami A, Geula C, Hsu WH, Horbinski C, James CD, Chen P. Nat Immunol 24: 2023 1654-1670	65
Reference-free inferring of transcriptomic events in cancer cells on single-cell data. Eralp B, Sefer E. BMC Cancer 24: 2024 607	27
Cellular diversity through space and time: adding new dimensions to GBM therapeutic development. Johnson AL, Lopez-Bertoni H. Front Genet 15: 2024 1356611	9
Mapping chromatin remodelling in glioblastoma identifies epigenetic regulation of key molecular pathways and novel druggable targets. Vinel C, Boot J, Jin W, Pomella N, Hadaway A, Mein C, Zabet NR, Marino S. BMC Biol 23: 2025 26	10
Programs, origins and immunomodulatory functions of myeloid cells in glioma. Miller TE, El Farran CA, Couturier CP, Chen Z, D'Antonio JP, Verga J, Villanueva MA, Gonzalez Castro LN, Tong YE, Saadi TA, Chiocca AN, Zhang Y, Fischer DS, Heiland DH, Guerriero JL, Petrecca K, Suva ML, Shalek AK, Bernstein BE. Nature 640: 2025 1072-1082	118
Targeting legumain-mediated cell-cell interaction sensitizes glioblastoma to immunotherapy in preclinical models. Pang L, Guo S, Huang Y, Khan F, Liu Y, Zhou F, Lathia JD, Chen P. J Clin Invest 135: 2025 e186034	14
Charting the Single-Cell and Spatial Landscape of IDH-Wildtype Glioblastoma with GBmap. Ruiz-Moreno C, Salas SM, Samuelsson E, Minaeva M, Ibarra I, Grillo M, Brandner S, Roy A, Forsberg-Nilsson K, Kranendonk MEG, Theis FJ, Nilsson M, Stunnenberg HG. Neuro Oncol 27: 2025 2281-2295	50
Glycoprotein NMB mediates bidirectional GSC-TAM interactions to promote tumor progression. Liu Y, Pang L, Khan F, Wu J, Zhou F, Horbinski C, Bao S, Yu JS, Lathia JD, Chen P. JCI Insight 10: 2025 e187684	7
GDF15 Reprograms the Microenvironment to Drive Liver Metastasis of Uveal Melanoma. Sriramareddy S, Siddarajappa N, Park JS, Nguyen T, Kuznetsoff JN, Kurtenbach S, Dollar JJ, Adams DJ, Licht JD, Correa ZM, Chen YA, Harbour JW, Smalley KSM. Cancer Res 86: 2026 3968-3986	2