Description and determinants of the faecal resistome and microbiome of farmers and slaughterhouse workers: a metagenome-wide cross-sectional study.

Within this study, we extracted DNA of 194 stool samples of persons living or working on pig and broiler farms (54 pig farmers, 24 broiler farmers), 70 pig slaughterhouse workers and 46 control subjects (Lifelines population living in the Northern parts of the Netherlands, and without recent antimicrobial use and occupational livestock contact). Total DNA was sequenced and metagenomic analysis was performed. Subsequently, the faecal resistomes and microbiomes were described and analysed (doi: 10.1016/j.envint.2020.105939). Highlights study: • Food chain workers are exposed to antimicrobial resistant (AMR) bacteria in livestock. • We describe faecal resistomes and microbiomes of farmers and slaughterhouse workers. • Faecal AMR gene loads in pork exposed workers exceed those in broiler farmers/controls. • Resistome and microbiome differences are shown between and within occupational groups. • On-farm working hours and working/living on a pig farm determine the faecal resistome. • Contact with livestock may be a determinant for human AMR gene carriage. This study is part of the EFFORT project: http://www.effort-against-amr.eu/.

Type: Other
Archiver: European Genome-Phenome Archive (EGA)

2 Datasets 2 Publications

Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data

Dataset ID	Description	Technology	Samples
EGAD00001005443	Metagenomes of stool samples from 46 Lifelines control subjects (no antimicrobial use in the past three months before sampling, no occupational lifestock contact). Samples were sequenced and analysed as part of the EFFORT project and derived from the LifeLines cohort from the Northern parts of the Netherlands. http:///www.lifelines.nl.	Illumina HiSeq 4000	46
EGAD00001005444	Metagenomes of stool samples from 54 pig farmers, 24 broiler farmers and 70 slaughter line workers. Note: Access to the data will only be granted for antibiotic resistance studies in accordance with the EFFORT consents issued by the participants.	Illumina HiSeq 4000	148

Publications	Citations
Description and determinants of the faecal resistome and microbiome of farmers and slaughterhouse workers: A metagenome-wide cross-sectional study. Van Gompel L, Luiken REC, Hansen RB, Munk P, Bouwknegt M, Heres L, Greve GD, Scherpenisse P, Jongerius-Gortemaker BGM, Tersteeg-Zijderveld MHG, García-Cobos S, Dohmen W, Dorado-García A, Wagenaar JA, Urlings BAP, Aarestrup FM, Mevius DJ, Heederik DJJ, Schmitt H, Bossers A, Smit LAM. Environ Int 143: 2020 105939	20
Metagenomics-Based Approach to Source-Attribution of Antimicrobial Resistance Determinants - Identification of Reservoir Resistome Signatures. Duarte ASR, Röder T, Van Gompel L, Petersen TN, Hansen RB, Hansen IM, Bossers A, Aarestrup FM, Wagenaar JA, Hald T. Front Microbiol 11: 2020 601407	22

Publications

Citations

Description and determinants of the faecal resistome and microbiome of farmers and slaughterhouse workers: A metagenome-wide cross-sectional study.
Van Gompel L, Luiken REC, Hansen RB, Munk P, Bouwknegt M, Heres L, Greve GD, Scherpenisse P, Jongerius-Gortemaker BGM, Tersteeg-Zijderveld MHG, García-Cobos S, Dohmen W, Dorado-García A, Wagenaar JA, Urlings BAP, Aarestrup FM, Mevius DJ, Heederik DJJ, Schmitt H, Bossers A, Smit LAM. Environ Int 143: 2020 105939

Metagenomics-Based Approach to Source-Attribution of Antimicrobial Resistance Determinants - Identification of Reservoir Resistome Signatures.
Duarte ASR, Röder T, Van Gompel L, Petersen TN, Hansen RB, Hansen IM, Bossers A, Aarestrup FM, Wagenaar JA, Hald T. Front Microbiol 11: 2020 601407