FASTQ files of total RNA-Seq data from the POPS SGA (Small for Gestational Age) samples

All the samples were obtained from the Pregnancy Outcome Prediction–a prospective cohort study of nulliparous women attending the Rosie Hospital, Cambridge (UK) for their dating ultrasound scan between January 14, 2008, and July 31, 2012. Ethical approval for the study was given by the Cambridgeshire 2 Research Ethics Committee (reference number 07/H0308/163) and all participants provided written informed consent. Cases of preeclampsia (PET) were defined on the basis of the 2013 ACOG criteria and cases of small for gestational age (SGA)infants were confined to severe SGA, i.e. a customized birth weight <5th percentile. Chorionic villi from the corresponding placentas (free from decidua, visible infarction, calcification, hematoma, or damage) were collected and processed within 30 minutes of separation from the uterus. After repeated washes in chilled phosphate buffered saline, the samples were placed in RNA later (Applied Biosystems) and stored at -80°C. Total placental RNA was extracted using mirVana Isolation Kit (Ambion). For each placenta, approximately 5 mg of tissue were homogenized in the Lysis/Binding solution for 20 sec at 6 m/s using a bead beater (FastPrep24) and Lysing Matrix D Tubes (MP Biomedicals). The samples were then spun at 13,000 rpm for 5 min at 4°C and the supernatants recovered. Afterwards, the manufacturer's instructions were followed. Immediately after the RNA extraction, placental RNA samples were DNase-treated using DNA-free DNA Removal Kit (Ambion), aliquoted, and stored in -80°C. Quantity and quality of the RNA samples were assessed using the Agilent 2100 Bioanalyzer, the Agilent RNA 6000 Nano Kit (Agilent Technologies), and Qubit fluorometer. Libraries were prepared starting with 300-500 ng of good quality total RNA (RIN ≥7.5) using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat (Illumina), according to the manufacturer's instructions. The kit contains 96 uniquely indexed adapter combinations in order to allow pooling of multiple samples prior to sequencing. After determining their size (with the Agilent 2100 Bioanalyzer and the Agilent High Sensitivity DNA Kit by Agilent Technologies) and concentration (by qPCR with the KAPA Illumina ABI Prism Library Quantification Kit, Kapa Biosystems), libraries have been pooled and sequenced (single-end, 125 bp) using a Single End V4 Cluster Kit and an Illumina HiSeq2500 or HiSeq4000 instrument.

52 samples
DAC: EGAC00001000582
Technology: Illumina HiSeq 4000

Access Policy1 Study Files

Metadata

Request Access

POPS data access policy

You are not authroised to access POPS data unless you have a written permission from the data access committee which you can contact below: D. Stephen Charnock-Jones. Professor of Reproductive Biology. Department of Obstetrics and Gynaecology. University of Cambridge. The Rosie Hospital, Cambridge. CB2 0SW UK email dscj1@cam.ac.uk Tel +44 1223 336875 Fax +44 1223 215327

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID	Study Title	Study Type
EGAS00001002205	Genomic data from analysis of the human placenta, part of the Pregnancy Outcome Prediction study (POPs)	Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID	File Type	Size
EGAF00001707733	fq.gz	283.1 MB
EGAF00001707734	fq.gz	282.8 MB
EGAF00001707735	fq.gz	283.2 MB
EGAF00001707736	fq.gz	264.1 MB
EGAF00001707737	fq.gz	335.0 MB
EGAF00001707738	fq.gz	333.8 MB
EGAF00001707739	fq.gz	331.5 MB
EGAF00001707740	fq.gz	333.3 MB
EGAF00001707741	fq.gz	333.9 MB
EGAF00001707742	fq.gz	337.2 MB
EGAF00001707743	fq.gz	337.4 MB
EGAF00001707744	fq.gz	312.7 MB
EGAF00001707745	fq.gz	306.0 MB
EGAF00001707746	fq.gz	307.1 MB
EGAF00001707747	fq.gz	311.6 MB
EGAF00001707748	fq.gz	312.9 MB
EGAF00001707749	fq.gz	308.6 MB
EGAF00001707750	fq.gz	310.1 MB
EGAF00001707751	fq.gz	311.7 MB
EGAF00001707752	fq.gz	413.4 MB
EGAF00001707753	fq.gz	420.3 MB
EGAF00001707754	fq.gz	416.2 MB
EGAF00001707755	fq.gz	395.3 MB
EGAF00001707756	fq.gz	370.2 MB
EGAF00001707757	fq.gz	372.8 MB
EGAF00001707758	fq.gz	373.2 MB
EGAF00001707759	fq.gz	373.5 MB
EGAF00001707760	fq.gz	371.4 MB
EGAF00001707761	fq.gz	373.3 MB
EGAF00001707762	fq.gz	373.0 MB
EGAF00001707763	fq.gz	347.1 MB
EGAF00001707764	fq.gz	402.6 MB
EGAF00001707765	fq.gz	394.6 MB
EGAF00001707766	fq.gz	391.6 MB
EGAF00001707767	fq.gz	384.1 MB
EGAF00001707768	fq.gz	390.6 MB
EGAF00001707769	fq.gz	392.2 MB
EGAF00001707770	fq.gz	393.7 MB
EGAF00001707771	fq.gz	363.9 MB
EGAF00001707772	fq.gz	365.2 MB
EGAF00001707773	fq.gz	365.4 MB
EGAF00001707774	fq.gz	342.4 MB
EGAF00001707775	fq.gz	356.4 MB
EGAF00001707776	fq.gz	352.9 MB
EGAF00001707777	fq.gz	353.5 MB
EGAF00001707778	fq.gz	352.5 MB
EGAF00001707779	fq.gz	351.0 MB
EGAF00001707780	fq.gz	356.1 MB
EGAF00001707781	fq.gz	352.7 MB
EGAF00001707782	fq.gz	334.9 MB
EGAF00001707783	fq.gz	325.6 MB
EGAF00001707784	fq.gz	325.0 MB
EGAF00001707785	fq.gz	325.4 MB
EGAF00001707786	fq.gz	325.6 MB
EGAF00001707787	fq.gz	323.8 MB
EGAF00001707788	fq.gz	325.6 MB
EGAF00001707789	fq.gz	325.3 MB
EGAF00001707790	fq.gz	368.2 MB
EGAF00001707791	fq.gz	368.0 MB
EGAF00001707792	fq.gz	344.2 MB
EGAF00001707793	fq.gz	358.1 MB
EGAF00001707794	fq.gz	354.6 MB
EGAF00001707795	fq.gz	355.9 MB
EGAF00001707796	fq.gz	355.3 MB
EGAF00001707797	fq.gz	353.3 MB
EGAF00001707798	fq.gz	358.7 MB
EGAF00001707799	fq.gz	355.5 MB
EGAF00001707800	fq.gz	336.1 MB
EGAF00001707847	fq.gz	432.2 MB
EGAF00001707848	fq.gz	549.3 MB
EGAF00001707849	fq.gz	547.5 MB
EGAF00001707850	fq.gz	546.9 MB
EGAF00001707851	fq.gz	550.3 MB
EGAF00001707852	fq.gz	549.9 MB
EGAF00001707853	fq.gz	555.6 MB
EGAF00001707854	fq.gz	555.3 MB
EGAF00001707855	fq.gz	514.4 MB
EGAF00001707856	fq.gz	501.2 MB
EGAF00001707857	fq.gz	502.7 MB
EGAF00001707858	fq.gz	510.7 MB
EGAF00001707859	fq.gz	512.2 MB
EGAF00001707860	fq.gz	507.3 MB
EGAF00001707861	fq.gz	510.9 MB
EGAF00001707862	fq.gz	510.5 MB
EGAF00001707863	fq.gz	472.1 MB
EGAF00001707864	fq.gz	414.3 MB
EGAF00001707865	fq.gz	415.4 MB
EGAF00001707866	fq.gz	288.4 MB
EGAF00001707867	fq.gz	324.0 MB
EGAF00001707868	fq.gz	323.2 MB
EGAF00001707869	fq.gz	323.5 MB
EGAF00001707870	fq.gz	322.9 MB
EGAF00001707871	fq.gz	323.2 MB
EGAF00001707872	fq.gz	323.2 MB
EGAF00001707873	fq.gz	322.9 MB
EGAF00001707874	fq.gz	301.8 MB
EGAF00001707875	fq.gz	383.5 MB
EGAF00001707876	fq.gz	381.7 MB
EGAF00001707877	fq.gz	378.9 MB
EGAF00001707878	fq.gz	381.0 MB
EGAF00001707879	fq.gz	382.3 MB
EGAF00001707880	fq.gz	385.4 MB
EGAF00001707881	fq.gz	386.4 MB
EGAF00001707882	fq.gz	358.5 MB
EGAF00001707883	fq.gz	350.0 MB
EGAF00001707884	fq.gz	350.9 MB
EGAF00001707885	fq.gz	371.0 MB
EGAF00001707886	fq.gz	393.2 MB
EGAF00001707887	fq.gz	388.2 MB
EGAF00001707888	fq.gz	388.0 MB
EGAF00001707889	fq.gz	386.2 MB
EGAF00001707890	fq.gz	383.7 MB
EGAF00001707891	fq.gz	389.5 MB
EGAF00001707892	fq.gz	386.4 MB
EGAF00001707893	fq.gz	367.7 MB
EGAF00001707894	fq.gz	360.4 MB
EGAF00001707895	fq.gz	359.7 MB
EGAF00001707896	fq.gz	359.9 MB
EGAF00001707897	fq.gz	360.1 MB
EGAF00001707898	fq.gz	358.2 MB
EGAF00001707899	fq.gz	360.5 MB
EGAF00001707900	fq.gz	359.7 MB
EGAF00001707901	fq.gz	336.9 MB
EGAF00001707902	fq.gz	402.5 MB
EGAF00001707903	fq.gz	400.5 MB
EGAF00001707961	fq.gz	415.5 MB
EGAF00001707962	fq.gz	415.3 MB
EGAF00001707963	fq.gz	415.9 MB
EGAF00001707964	fq.gz	415.6 MB
EGAF00001707965	fq.gz	415.4 MB
EGAF00001707966	fq.gz	387.5 MB
EGAF00001707967	fq.gz	489.6 MB
EGAF00001707968	fq.gz	488.9 MB
EGAF00001707969	fq.gz	487.4 MB
EGAF00001707970	fq.gz	489.8 MB
EGAF00001707971	fq.gz	490.1 MB
EGAF00001707972	fq.gz	494.9 MB
EGAF00001707973	fq.gz	495.0 MB
EGAF00001707974	fq.gz	458.3 MB
EGAF00001707975	fq.gz	446.6 MB
EGAF00001707976	fq.gz	448.9 MB
EGAF00001707977	fq.gz	456.0 MB
EGAF00001707978	fq.gz	457.5 MB
EGAF00001707979	fq.gz	452.6 MB
EGAF00001707980	fq.gz	356.2 MB
EGAF00001707981	fq.gz	357.6 MB
EGAF00001707982	fq.gz	352.9 MB
EGAF00001707983	fq.gz	355.0 MB
EGAF00001707984	fq.gz	355.6 MB
EGAF00001707985	fq.gz	329.8 MB
EGAF00001707986	fq.gz	326.3 MB
EGAF00001707987	fq.gz	326.5 MB
EGAF00001707988	fq.gz	327.0 MB
EGAF00001707989	fq.gz	325.9 MB
EGAF00001707990	fq.gz	326.3 MB
EGAF00001707991	fq.gz	326.3 MB
EGAF00001707992	fq.gz	326.5 MB
EGAF00001707993	fq.gz	304.5 MB
EGAF00001707994	fq.gz	386.6 MB
EGAF00001707995	fq.gz	385.4 MB
EGAF00001707996	fq.gz	383.9 MB
EGAF00001707997	fq.gz	386.1 MB
EGAF00001707998	fq.gz	386.5 MB
EGAF00001707999	fq.gz	400.3 MB
EGAF00001708000	fq.gz	392.6 MB
EGAF00001708001	fq.gz	400.3 MB
EGAF00001708002	fq.gz	401.0 MB
EGAF00001708003	fq.gz	400.1 MB
EGAF00001708004	fq.gz	372.1 MB
EGAF00001708005	fq.gz	382.6 MB
EGAF00001708006	fq.gz	379.5 MB
EGAF00001708007	fq.gz	380.8 MB
EGAF00001708008	fq.gz	381.0 MB
EGAF00001708009	fq.gz	379.2 MB
EGAF00001708010	fq.gz	384.5 MB
EGAF00001708011	fq.gz	380.5 MB
EGAF00001708012	fq.gz	359.7 MB
EGAF00001708013	fq.gz	401.2 MB
EGAF00001708014	fq.gz	403.4 MB
EGAF00001708015	fq.gz	404.0 MB
EGAF00001708016	fq.gz	404.5 MB
EGAF00001708017	fq.gz	401.6 MB
EGAF00001708075	fq.gz	455.6 MB
EGAF00001708076	fq.gz	456.8 MB
EGAF00001708077	fq.gz	421.2 MB
EGAF00001708078	fq.gz	400.1 MB
EGAF00001708079	fq.gz	400.1 MB
EGAF00001708080	fq.gz	400.6 MB
EGAF00001708081	fq.gz	400.1 MB
EGAF00001708082	fq.gz	400.2 MB
EGAF00001708083	fq.gz	399.5 MB
EGAF00001708084	fq.gz	399.9 MB
EGAF00001708085	fq.gz	373.0 MB
EGAF00001708086	fq.gz	473.9 MB
EGAF00001708087	fq.gz	472.3 MB
EGAF00001708088	fq.gz	470.5 MB
EGAF00001708089	fq.gz	473.5 MB
EGAF00001708090	fq.gz	473.3 MB
EGAF00001708091	fq.gz	478.1 MB
EGAF00001708092	fq.gz	478.8 MB
EGAF00001708093	fq.gz	443.8 MB
EGAF00001708094	fq.gz	389.8 MB
EGAF00001708095	fq.gz	390.2 MB
EGAF00001708096	fq.gz	361.9 MB
EGAF00001708097	fq.gz	352.8 MB
EGAF00001708098	fq.gz	354.3 MB
EGAF00001708099	fq.gz	359.6 MB
EGAF00001708100	fq.gz	360.8 MB
EGAF00001708101	fq.gz	356.5 MB
EGAF00001708102	fq.gz	358.5 MB
EGAF00001708103	fq.gz	358.9 MB
EGAF00001708104	fq.gz	332.4 MB
EGAF00001708105	fq.gz	370.6 MB
EGAF00001708106	fq.gz	369.4 MB
EGAF00001708107	fq.gz	369.5 MB
EGAF00001708108	fq.gz	368.3 MB
EGAF00001708109	fq.gz	368.9 MB
EGAF00001708110	fq.gz	368.3 MB
EGAF00001708111	fq.gz	369.3 MB
EGAF00001708112	fq.gz	344.3 MB
EGAF00001708113	fq.gz	404.5 MB
EGAF00001708114	fq.gz	403.8 MB
EGAF00001708115	fq.gz	376.6 MB
EGAF00001708116	fq.gz	439.2 MB
EGAF00001708117	fq.gz	430.4 MB
EGAF00001708118	fq.gz	428.5 MB
EGAF00001708119	fq.gz	420.2 MB
EGAF00001708120	fq.gz	427.3 MB
EGAF00001708121	fq.gz	429.5 MB
EGAF00001708122	fq.gz	429.9 MB
EGAF00001708123	fq.gz	404.3 MB
EGAF00001708124	fq.gz	426.6 MB
EGAF00001708125	fq.gz	421.1 MB
EGAF00001708126	fq.gz	421.3 MB
EGAF00001708127	fq.gz	420.3 MB
EGAF00001708128	fq.gz	417.2 MB
EGAF00001708129	fq.gz	423.5 MB
EGAF00001708130	fq.gz	419.8 MB
EGAF00001708131	fq.gz	398.8 MB
EGAF00001708189	fq.gz	433.1 MB
EGAF00001708190	fq.gz	434.3 MB
EGAF00001708191	fq.gz	440.9 MB
EGAF00001708192	fq.gz	442.6 MB
EGAF00001708193	fq.gz	437.5 MB
EGAF00001708194	fq.gz	441.0 MB
EGAF00001708195	fq.gz	440.7 MB
EGAF00001708196	fq.gz	407.7 MB
EGAF00001708197	fq.gz	386.7 MB
EGAF00001708198	fq.gz	386.2 MB
EGAF00001708199	fq.gz	386.3 MB
EGAF00001708200	fq.gz	385.8 MB
EGAF00001708201	fq.gz	386.1 MB
EGAF00001708202	fq.gz	385.3 MB
EGAF00001708203	fq.gz	386.2 MB
EGAF00001708204	fq.gz	360.7 MB
EGAF00001708205	fq.gz	458.8 MB
EGAF00001708206	fq.gz	456.8 MB
EGAF00001708207	fq.gz	454.8 MB
EGAF00001708208	fq.gz	441.4 MB
EGAF00001708209	fq.gz	438.9 MB
EGAF00001708210	fq.gz	435.1 MB
EGAF00001708211	fq.gz	437.5 MB
EGAF00001708212	fq.gz	438.0 MB
EGAF00001708213	fq.gz	442.2 MB
EGAF00001708214	fq.gz	443.0 MB
EGAF00001708215	fq.gz	411.5 MB
EGAF00001708216	fq.gz	403.6 MB
EGAF00001708217	fq.gz	403.9 MB
EGAF00001708218	fq.gz	409.3 MB
EGAF00001708219	fq.gz	410.1 MB
EGAF00001708220	fq.gz	404.3 MB
EGAF00001708221	fq.gz	406.3 MB
EGAF00001708222	fq.gz	407.8 MB
EGAF00001708223	fq.gz	379.0 MB
EGAF00001708224	fq.gz	346.2 MB
EGAF00001708225	fq.gz	345.4 MB
EGAF00001708226	fq.gz	345.2 MB
EGAF00001708227	fq.gz	503.5 MB
EGAF00001708228	fq.gz	506.3 MB
EGAF00001708229	fq.gz	507.1 MB
EGAF00001708230	fq.gz	507.9 MB
EGAF00001708231	fq.gz	504.5 MB
EGAF00001708232	fq.gz	507.4 MB
EGAF00001708233	fq.gz	506.2 MB
EGAF00001708234	fq.gz	471.6 MB
EGAF00001708235	fq.gz	556.0 MB
EGAF00001708236	fq.gz	546.0 MB
EGAF00001708237	fq.gz	542.7 MB
EGAF00001708238	fq.gz	530.4 MB
EGAF00001708239	fq.gz	541.1 MB
EGAF00001708240	fq.gz	544.5 MB
EGAF00001708241	fq.gz	545.6 MB
EGAF00001708242	fq.gz	512.5 MB
EGAF00001708243	fq.gz	537.7 MB
EGAF00001708244	fq.gz	532.5 MB
EGAF00001708245	fq.gz	532.8 MB
EGAF00001708246	fq.gz	304.1 MB
EGAF00001708247	fq.gz	364.7 MB
EGAF00001708248	fq.gz	362.1 MB
EGAF00001708249	fq.gz	361.9 MB
EGAF00001708250	fq.gz	353.9 MB
EGAF00001708251	fq.gz	361.0 MB
EGAF00001708252	fq.gz	362.1 MB
EGAF00001708253	fq.gz	362.4 MB
EGAF00001708254	fq.gz	336.4 MB
EGAF00001708255	fq.gz	346.4 MB
EGAF00001708256	fq.gz	344.1 MB
EGAF00001708257	fq.gz	345.1 MB
EGAF00001708258	fq.gz	344.9 MB
EGAF00001708316	fq.gz	457.8 MB
EGAF00001708317	fq.gz	457.8 MB
EGAF00001708318	fq.gz	461.7 MB
EGAF00001708319	fq.gz	462.4 MB
EGAF00001708320	fq.gz	429.4 MB
EGAF00001708321	fq.gz	419.3 MB
EGAF00001708322	fq.gz	420.4 MB
EGAF00001708323	fq.gz	426.2 MB
EGAF00001708324	fq.gz	427.7 MB
EGAF00001708325	fq.gz	422.7 MB
EGAF00001708326	fq.gz	425.2 MB
EGAF00001708327	fq.gz	425.6 MB
EGAF00001708328	fq.gz	394.4 MB
EGAF00001708329	fq.gz	335.0 MB
EGAF00001708330	fq.gz	334.0 MB
EGAF00001708331	fq.gz	333.8 MB
EGAF00001708332	fq.gz	333.6 MB
EGAF00001708333	fq.gz	334.3 MB
EGAF00001708334	fq.gz	333.5 MB
EGAF00001708335	fq.gz	345.1 MB
EGAF00001708336	fq.gz	345.4 MB
EGAF00001708337	fq.gz	345.1 MB
EGAF00001708338	fq.gz	345.7 MB
EGAF00001708339	fq.gz	322.5 MB
EGAF00001708340	fq.gz	410.5 MB
EGAF00001708341	fq.gz	408.4 MB
EGAF00001708342	fq.gz	405.3 MB
EGAF00001708343	fq.gz	407.3 MB
EGAF00001708344	fq.gz	408.1 MB
EGAF00001708345	fq.gz	412.4 MB
EGAF00001708346	fq.gz	412.0 MB
EGAF00001708347	fq.gz	382.9 MB
EGAF00001708348	fq.gz	375.6 MB
EGAF00001708349	fq.gz	376.2 MB
EGAF00001708350	fq.gz	381.4 MB
EGAF00001708351	fq.gz	382.2 MB
EGAF00001708352	fq.gz	377.1 MB
EGAF00001708353	fq.gz	379.0 MB
EGAF00001708354	fq.gz	531.8 MB
EGAF00001708355	fq.gz	528.1 MB
EGAF00001708356	fq.gz	536.7 MB
EGAF00001708357	fq.gz	531.5 MB
EGAF00001708358	fq.gz	503.8 MB
EGAF00001708359	fq.gz	416.8 MB
EGAF00001708360	fq.gz	421.7 MB
EGAF00001708361	fq.gz	423.0 MB
EGAF00001708362	fq.gz	423.5 MB
EGAF00001708363	fq.gz	420.3 MB
EGAF00001708364	fq.gz	422.5 MB
EGAF00001708365	fq.gz	421.9 MB
EGAF00001708366	fq.gz	392.4 MB
EGAF00001708367	fq.gz	443.5 MB
EGAF00001708368	fq.gz	429.1 MB
EGAF00001708369	fq.gz	425.2 MB
EGAF00001708370	fq.gz	413.7 MB
EGAF00001708371	fq.gz	423.4 MB
EGAF00001708372	fq.gz	425.8 MB
EGAF00001708373	fq.gz	343.1 MB
EGAF00001708374	fq.gz	348.0 MB
EGAF00001708375	fq.gz	344.4 MB
EGAF00001708376	fq.gz	325.9 MB
EGAF00001708377	fq.gz	327.7 MB
EGAF00001708378	fq.gz	328.0 MB
EGAF00001708379	fq.gz	328.8 MB
EGAF00001708380	fq.gz	328.6 MB
EGAF00001708381	fq.gz	327.0 MB
EGAF00001708382	fq.gz	329.1 MB
EGAF00001708383	fq.gz	328.2 MB
EGAF00001708384	fq.gz	306.8 MB
EGAF00001708385	fq.gz	365.2 MB
EGAF00001708386	fq.gz	362.7 MB
EGAF00001708387	fq.gz	362.2 MB
EGAF00001708388	fq.gz	354.6 MB
EGAF00001708389	fq.gz	361.5 MB
EGAF00001708390	fq.gz	362.8 MB
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EGAF00001709770	fq.gz	368.3 MB
EGAF00001709771	fq.gz	367.6 MB
EGAF00001709772	fq.gz	359.2 MB
EGAF00001709773	fq.gz	366.8 MB
1248 Files (479.6 GB)