WGoxBS and SureSelect data of the POPS placenta samples
We carried out whole-genome oxidative bisulfite sequencing (WGoxBS) in the placentas of two healthy female and two healthy male pregnancies generating an average genome depth of coverage of 25x. The sex-specific differential methylation pattern observed in this region was validated in additional 8 healthy placentas (including 2 from the WGoxBS) using SureSelect in-solution target capture. For WGoxBS, placental genomic DNA (4 µg) from 4 healthy pregnancies was processed to achieve 10 kb fragments with the g-Tube (Covaris), according to the manufacturer's instructions. To increase the number of uniquely sequenced reads, two independent libraries were generated for each individual. Multiplexed sequencing was carried out on the Illumina MiSeq, HiSeq 2000, and HiSeq 2500 instruments with 2x100, 2x50 and 2x125 cycles using MiSeq Reagent Kit v3, HiSeq SBS Kit v3 and HiSeq SBS Kit v4, respectively. For SureSelect in-solution capture, placental genomic DNA (3.5 µg) from 8 healthy pregnancies (including 2 from the WGoxBS) was fragmented by the Covaris S220 system according to the SureSelect Methyl-Seq target enrichment protocol (Agilent). All 8 libraries were pooled and sequenced on the Illumina HiSeq 2500 instrument with 2 × 125 cycles using HiSeq SBS Kit v4 and a single lane of the Illumina HiSeq 4000 instrument with 2 × 150 cycles using HiSeq 3000/4000 SBS Kit following Illumina's guidelines (Illumina Application Note: Epigenetics February 2016).
- 10 samples
- DAC: EGAC00001000582
- Technology: Illumina HiSeq 2500
- HMB DUO:0000006 (version: 2019-01-07)health or medical or biomedical researchThis data use permission indicates that use is allowed for health/medical/biomedical purposes; does not include the study of population origins or ancestry.
- US DUO:0000026 (version: 2019-01-07)user specific restrictionThis data use modifier indicates that use is limited to use by approved users.
POPS data access policy
You are not authroised to access POPS data unless you have a written permission from the data access committee which you can contact below: D. Stephen Charnock-Jones. Professor of Reproductive Biology. Department of Obstetrics and Gynaecology. University of Cambridge. The Rosie Hospital, Cambridge. CB2 0SW UK email dscj1@cam.ac.uk Tel +44 1223 336875 Fax +44 1223 215327
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| Other |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF00001433440 | fq.gz | 217.3 MB | ||
| EGAF00001433441 | fq.gz | 235.7 MB | ||
| EGAF00001433442 | fq.gz | 1.5 GB | ||
| EGAF00001433443 | fq.gz | 1.4 GB | ||
| EGAF00001433444 | fq.gz | 1.5 GB | ||
| EGAF00001433445 | fq.gz | 1.4 GB | ||
| EGAF00001433446 | fq.gz | 1.5 GB | ||
| EGAF00001433447 | fq.gz | 1.4 GB | ||
| EGAF00001433448 | fq.gz | 1.5 GB | ||
| EGAF00001433449 | fq.gz | 1.4 GB | ||
| EGAF00001433450 | fq.gz | 1.4 GB | ||
| EGAF00001433451 | fq.gz | 1.4 GB | ||
| EGAF00001433452 | fq.gz | 1.6 GB | ||
| EGAF00001433453 | fq.gz | 1.5 GB | ||
| EGAF00001433454 | fq.gz | 1.5 GB | ||
| EGAF00001433455 | fq.gz | 1.4 GB | ||
| EGAF00001433456 | fq.gz | 1.5 GB | ||
| EGAF00001433457 | fq.gz | 1.4 GB | ||
| EGAF00001433458 | fq.gz | 2.4 GB | ||
| EGAF00001433459 | fq.gz | 2.3 GB | ||
| EGAF00001433460 | fq.gz | 2.3 GB | ||
| EGAF00001433461 | fq.gz | 2.3 GB | ||
| EGAF00001433462 | fq.gz | 2.3 GB | ||
| EGAF00001433463 | fq.gz | 2.3 GB | ||
| EGAF00001433464 | fq.gz | 2.4 GB | ||
| EGAF00001433465 | fq.gz | 2.3 GB | ||
| EGAF00001433466 | fq.gz | 2.3 GB | ||
| EGAF00001433467 | fq.gz | 2.3 GB | ||
| EGAF00001433468 | fq.gz | 2.3 GB | ||
| EGAF00001433469 | fq.gz | 2.3 GB | ||
| EGAF00001433470 | fq.gz | 2.4 GB | ||
| EGAF00001433471 | fq.gz | 2.3 GB | ||
| EGAF00001433472 | fq.gz | 2.3 GB | ||
| EGAF00001433473 | fq.gz | 2.2 GB | ||
| EGAF00001433474 | fq.gz | 322.0 MB | ||
| EGAF00001433475 | fq.gz | 354.9 MB | ||
| EGAF00001433476 | fq.gz | 4.1 GB | ||
| EGAF00001433477 | fq.gz | 4.6 GB | ||
| EGAF00001433478 | fq.gz | 4.2 GB | ||
| EGAF00001433479 | fq.gz | 4.6 GB | ||
| EGAF00001433480 | fq.gz | 4.2 GB | ||
| EGAF00001433481 | fq.gz | 4.6 GB | ||
| EGAF00001433482 | fq.gz | 4.3 GB | ||
| EGAF00001433483 | fq.gz | 4.7 GB | ||
| EGAF00001433484 | fq.gz | 4.3 GB | ||
| EGAF00001433485 | fq.gz | 4.7 GB | ||
| EGAF00001433486 | fq.gz | 4.2 GB | ||
| EGAF00001433487 | fq.gz | 4.6 GB | ||
| EGAF00001433488 | fq.gz | 4.2 GB | ||
| EGAF00001433489 | fq.gz | 4.6 GB | ||
| EGAF00001433490 | fq.gz | 3.9 GB | ||
| EGAF00001433491 | fq.gz | 4.3 GB | ||
| EGAF00001433492 | fq.gz | 318.2 MB | ||
| EGAF00001433493 | fq.gz | 345.3 MB | ||
| EGAF00001433494 | fq.gz | 2.4 GB | ||
| EGAF00001433495 | fq.gz | 2.2 GB | ||
| EGAF00001433496 | fq.gz | 2.4 GB | ||
| EGAF00001433497 | fq.gz | 2.3 GB | ||
| EGAF00001433498 | fq.gz | 2.4 GB | ||
| EGAF00001433499 | fq.gz | 2.2 GB | ||
| EGAF00001433500 | fq.gz | 2.4 GB | ||
| EGAF00001433501 | fq.gz | 2.2 GB | ||
| EGAF00001433502 | fq.gz | 2.3 GB | ||
| EGAF00001433503 | fq.gz | 2.1 GB | ||
| EGAF00001433504 | fq.gz | 2.5 GB | ||
| EGAF00001433505 | fq.gz | 2.4 GB | ||
| EGAF00001433506 | fq.gz | 2.4 GB | ||
| EGAF00001433507 | fq.gz | 2.2 GB | ||
| EGAF00001433508 | fq.gz | 2.3 GB | ||
| EGAF00001433509 | fq.gz | 2.2 GB | ||
| EGAF00001433510 | fq.gz | 4.0 GB | ||
| EGAF00001433511 | fq.gz | 3.9 GB | ||
| EGAF00001433512 | fq.gz | 4.0 GB | ||
| EGAF00001433513 | fq.gz | 3.9 GB | ||
| EGAF00001433514 | fq.gz | 3.9 GB | ||
| EGAF00001433515 | fq.gz | 3.9 GB | ||
| EGAF00001433516 | fq.gz | 4.0 GB | ||
| EGAF00001433517 | fq.gz | 4.0 GB | ||
| EGAF00001433518 | fq.gz | 4.0 GB | ||
| EGAF00001433519 | fq.gz | 3.9 GB | ||
| EGAF00001433520 | fq.gz | 4.0 GB | ||
| EGAF00001433521 | fq.gz | 4.0 GB | ||
| EGAF00001433522 | fq.gz | 4.0 GB | ||
| EGAF00001433523 | fq.gz | 4.0 GB | ||
| EGAF00001433524 | fq.gz | 3.9 GB | ||
| EGAF00001433525 | fq.gz | 3.8 GB | ||
| EGAF00001433526 | fq.gz | 182.3 MB | ||
| EGAF00001433527 | fq.gz | 201.7 MB | ||
| EGAF00001433528 | fq.gz | 2.4 GB | ||
| EGAF00001433529 | fq.gz | 2.7 GB | ||
| EGAF00001433530 | fq.gz | 2.5 GB | ||
| EGAF00001433531 | fq.gz | 2.8 GB | ||
| EGAF00001433532 | fq.gz | 2.5 GB | ||
| EGAF00001433533 | fq.gz | 2.7 GB | ||
| EGAF00001433534 | fq.gz | 2.5 GB | ||
| EGAF00001433535 | fq.gz | 2.8 GB | ||
| EGAF00001433536 | fq.gz | 2.5 GB | ||
| EGAF00001433537 | fq.gz | 2.8 GB | ||
| EGAF00001433538 | fq.gz | 2.5 GB | ||
| EGAF00001433539 | fq.gz | 2.7 GB | ||
| EGAF00001433540 | fq.gz | 2.5 GB | ||
| EGAF00001433541 | fq.gz | 2.8 GB | ||
| EGAF00001433542 | fq.gz | 2.3 GB | ||
| EGAF00001433543 | fq.gz | 2.5 GB | ||
| EGAF00001433544 | fq.gz | 1.7 GB | ||
| EGAF00001433545 | fq.gz | 1.8 GB | ||
| EGAF00001433546 | fq.gz | 2.5 GB | ||
| EGAF00001433547 | fq.gz | 2.9 GB | ||
| EGAF00001433548 | fq.gz | 4.3 GB | ||
| EGAF00001433549 | fq.gz | 4.1 GB | ||
| EGAF00001433550 | fq.gz | 4.3 GB | ||
| EGAF00001433551 | fq.gz | 4.1 GB | ||
| EGAF00001433552 | fq.gz | 4.3 GB | ||
| EGAF00001433553 | fq.gz | 4.2 GB | ||
| EGAF00001433554 | fq.gz | 4.2 GB | ||
| EGAF00001433555 | fq.gz | 4.0 GB | ||
| EGAF00001433556 | fq.gz | 4.3 GB | ||
| EGAF00001433557 | fq.gz | 4.1 GB | ||
| EGAF00001433558 | fq.gz | 4.3 GB | ||
| EGAF00001433559 | fq.gz | 4.1 GB | ||
| EGAF00001433560 | fq.gz | 4.3 GB | ||
| EGAF00001433561 | fq.gz | 4.1 GB | ||
| EGAF00001433562 | fq.gz | 3.8 GB | ||
| EGAF00001433563 | fq.gz | 3.6 GB | ||
| EGAF00001433564 | fq.gz | 4.4 GB | ||
| EGAF00001433565 | fq.gz | 4.2 GB | ||
| EGAF00001433566 | fq.gz | 4.5 GB | ||
| EGAF00001433567 | fq.gz | 4.3 GB | ||
| EGAF00001433568 | fq.gz | 400.5 MB | ||
| EGAF00001433569 | fq.gz | 429.3 MB | ||
| EGAF00001433570 | fq.gz | 6.3 GB | ||
| EGAF00001433571 | fq.gz | 6.6 GB | ||
| EGAF00001433572 | fq.gz | 6.2 GB | ||
| EGAF00001433573 | fq.gz | 6.5 GB | ||
| EGAF00001433574 | fq.gz | 6.1 GB | ||
| EGAF00001433575 | fq.gz | 6.4 GB | ||
| EGAF00001433576 | fq.gz | 6.3 GB | ||
| EGAF00001433577 | fq.gz | 6.5 GB | ||
| EGAF00001433578 | fq.gz | 6.3 GB | ||
| EGAF00001433579 | fq.gz | 6.4 GB | ||
| EGAF00001433580 | fq.gz | 6.3 GB | ||
| EGAF00001433581 | fq.gz | 6.5 GB | ||
| EGAF00001433582 | fq.gz | 6.4 GB | ||
| EGAF00001433583 | fq.gz | 6.5 GB | ||
| EGAF00001433584 | fq.gz | 5.4 GB | ||
| EGAF00001433585 | fq.gz | 5.5 GB | ||
| EGAF00001433586 | fq.gz | 1.3 GB | ||
| EGAF00001433587 | fq.gz | 1.4 GB | ||
| EGAF00001433588 | fq.gz | 1.8 GB | ||
| EGAF00001433589 | fq.gz | 2.0 GB | ||
| EGAF00001433590 | fq.gz | 165.1 MB | ||
| EGAF00001433591 | fq.gz | 179.5 MB | ||
| EGAF00001433592 | fq.gz | 1.2 GB | ||
| EGAF00001433593 | fq.gz | 1.2 GB | ||
| EGAF00001433594 | fq.gz | 1.3 GB | ||
| EGAF00001433595 | fq.gz | 1.2 GB | ||
| EGAF00001433596 | fq.gz | 1.2 GB | ||
| EGAF00001433597 | fq.gz | 1.2 GB | ||
| EGAF00001433598 | fq.gz | 1.2 GB | ||
| EGAF00001433599 | fq.gz | 1.2 GB | ||
| EGAF00001433600 | fq.gz | 1.2 GB | ||
| EGAF00001433601 | fq.gz | 1.1 GB | ||
| EGAF00001433602 | fq.gz | 1.3 GB | ||
| EGAF00001433603 | fq.gz | 1.2 GB | ||
| EGAF00001433604 | fq.gz | 1.2 GB | ||
| EGAF00001433605 | fq.gz | 1.2 GB | ||
| EGAF00001433606 | fq.gz | 1.2 GB | ||
| EGAF00001433607 | fq.gz | 1.1 GB | ||
| EGAF00001433608 | fq.gz | 2.0 GB | ||
| EGAF00001433609 | fq.gz | 2.0 GB | ||
| EGAF00001433610 | fq.gz | 2.0 GB | ||
| EGAF00001433611 | fq.gz | 2.0 GB | ||
| EGAF00001433612 | fq.gz | 2.0 GB | ||
| EGAF00001433613 | fq.gz | 1.9 GB | ||
| EGAF00001433614 | fq.gz | 2.0 GB | ||
| EGAF00001433615 | fq.gz | 2.0 GB | ||
| EGAF00001433616 | fq.gz | 2.0 GB | ||
| EGAF00001433617 | fq.gz | 2.0 GB | ||
| EGAF00001433618 | fq.gz | 2.0 GB | ||
| EGAF00001433619 | fq.gz | 2.0 GB | ||
| EGAF00001433620 | fq.gz | 2.0 GB | ||
| EGAF00001433621 | fq.gz | 2.0 GB | ||
| EGAF00001433622 | fq.gz | 2.0 GB | ||
| EGAF00001433623 | fq.gz | 1.9 GB | ||
| EGAF00001433624 | fq.gz | 246.6 MB | ||
| EGAF00001433625 | fq.gz | 271.9 MB | ||
| EGAF00001433626 | fq.gz | 3.3 GB | ||
| EGAF00001433627 | fq.gz | 3.7 GB | ||
| EGAF00001433628 | fq.gz | 3.3 GB | ||
| EGAF00001433629 | fq.gz | 3.7 GB | ||
| EGAF00001433630 | fq.gz | 3.3 GB | ||
| EGAF00001433631 | fq.gz | 3.7 GB | ||
| EGAF00001433632 | fq.gz | 3.4 GB | ||
| EGAF00001433633 | fq.gz | 3.8 GB | ||
| EGAF00001433634 | fq.gz | 3.4 GB | ||
| EGAF00001433635 | fq.gz | 3.8 GB | ||
| EGAF00001433636 | fq.gz | 3.3 GB | ||
| EGAF00001433637 | fq.gz | 3.7 GB | ||
| EGAF00001433638 | fq.gz | 3.3 GB | ||
| EGAF00001433639 | fq.gz | 3.7 GB | ||
| EGAF00001433640 | fq.gz | 3.1 GB | ||
| EGAF00001433641 | fq.gz | 3.5 GB | ||
| EGAF00001433767 | fq.gz | 685.0 MB | ||
| EGAF00001433768 | fq.gz | 727.3 MB | ||
| EGAF00001433769 | fq.gz | 775.4 MB | ||
| EGAF00001433770 | fq.gz | 905.0 MB | ||
| EGAF00001433771 | fq.gz | 889.1 MB | ||
| EGAF00001433772 | fq.gz | 945.1 MB | ||
| EGAF00001433773 | fq.gz | 1.2 GB | ||
| EGAF00001433774 | fq.gz | 1.4 GB | ||
| EGAF00001433775 | fq.gz | 1.1 GB | ||
| EGAF00001433776 | fq.gz | 1.1 GB | ||
| EGAF00001433777 | fq.gz | 1.3 GB | ||
| EGAF00001433778 | fq.gz | 1.5 GB | ||
| EGAF00001433779 | fq.gz | 693.3 MB | ||
| EGAF00001433780 | fq.gz | 745.2 MB | ||
| EGAF00001433781 | fq.gz | 955.7 MB | ||
| EGAF00001433782 | fq.gz | 1.1 GB | ||
| EGAF00001433783 | fq.gz | 1.1 GB | ||
| EGAF00001433784 | fq.gz | 1.2 GB | ||
| EGAF00001433785 | fq.gz | 1.6 GB | ||
| EGAF00001433786 | fq.gz | 1.8 GB | ||
| EGAF00001433787 | fq.gz | 940.0 MB | ||
| EGAF00001433788 | fq.gz | 1.0 GB | ||
| EGAF00001433789 | fq.gz | 1.2 GB | ||
| EGAF00001433790 | fq.gz | 1.4 GB | ||
| EGAF00001433791 | bam | 79.5 GB | ||
| EGAF00001433792 | bam | 88.2 GB | ||
| EGAF00001433793 | bam | 64.3 GB | ||
| EGAF00001433794 | bam | 75.0 GB | ||
| EGAF00001433795 | bam | 9.3 GB | ||
| EGAF00001433796 | bam | 3.4 GB | ||
| EGAF00001433797 | bam | 6.9 GB | ||
| EGAF00001433798 | bam | 4.7 GB | ||
| EGAF00001433799 | bam | 5.3 GB | ||
| EGAF00001433800 | bam | 3.8 GB | ||
| EGAF00001433801 | bam | 6.3 GB | ||
| EGAF00001433802 | bam | 4.8 GB | ||
| 238 Files (960.2 GB) | ||||