Interrogating Gene Regulation During Human Cortical Development

Our study investigates the regulatory function and evolutionary origins of the fastest evolved genomic regions in the human genome termed Human Ancestor Quickly Evolved Regions (HAQERs), during human cortical development. Single-cell RNA sequencing (scRNA-seq) data were generated from human cortical progenitor cells from 4 individuals spanning the second trimester of development, subjected to pooled CRISPR interference (CRISPRi) perturbations targeting 50 HAQERs using an all-in-one lentiviral vector. Bulk ATAC-seq was performed using the same 4 human subjects to prioritize the 50 accessible HAQERs to be silenced. Single cell RNA sequencing libraries were generated using the 10x Genomics chromium high-throughput 3′ gene expression platform under lentiviral transduction with high MOI, low MOI, and low MOI under Wnt pathway stimulation. To enable assignment of individual donors in multiplexed single-cell experiments, genotypes were obtained using the Illumina Global Screening Array.

• Type: Epigenetics
• Archive: The database of Genotypes and Phenotypes (dbGaP)