Resolving the immune landscape of human prostate at a single cell level in health and cancer
The prostate gland produces prostatic fluid, high in zinc and citrate and essential for the maintenance of spermatozoa. Prostate cancer is a common condition with limited treatment efficacy in castration-resistant metastatic disease, including with immune checkpoint inhibitors. We used single-cell RNA-sequencing to perform an unbiased assessment of the cellular landscape of human prostate and identified a previously unappreciated subset of tumour-enriched androgen receptor-negative luminal epithelial cells, with increased expression of cancer-associated genes. We also found a variety of innate and adaptive immune cells in normal prostate that were transcriptionally perturbed in prostate cancer. An exception was a unique, prostate-specific, zinc transporter-expressing macrophage population (MAC-MT), that contributed to tissue zinc accumulation in homeostasis, but showed enhanced inflammatory gene expression in tumours, including T cell-recruiting chemokines. Remarkably, enrichment of the MAC-MT signature in cancer biopsies was associated with improved disease-free survival, suggesting beneficial anti-tumour functions.
- Type: Other
- Archiver: European Genome-Phenome Archive (EGA)
Click on a Dataset ID in the table below to learn more, and to find out who to contact about access to these data
Dataset ID | Description | Technology | Samples |
---|---|---|---|
EGAD00001008340 | Illumina HiSeq 4000 | 24 |
Publications | Citations |
---|---|
Resolving the immune landscape of human prostate at a single-cell level in health and cancer.
Cell Rep 37: 2021 110132 |
42 |
Master Transcription Factor Reprogramming Unleashes Selective Translation Promoting Castration Resistance and Immune Evasion in Lethal Prostate Cancer.
Cancer Discov 13: 2023 2584-2609 |
2 |
scRank infers drug-responsive cell types from untreated scRNA-seq data using a target-perturbed gene regulatory network.
Cell Rep Med 5: 2024 101568 |
0 |
Metabolic imaging across scales reveals distinct prostate cancer phenotypes.
Nat Commun 15: 2024 5980 |
0 |