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Transcriptomes of human CD4+ T lymphocytes - Metabolic project

Human CD4+ T cells are essential mediators of immune responses. By altering the mitochondrial and metabolic states, we defined metabolic requirements of human CD4+ T cells for in vitro activation, expansion, and effector function. T cell activation and proliferation were reduced by inhibiting oxidative phosphorylation, while early cytokine production was maintained by either OXPHOS or glycolytic activity. Glucose deprivation in the presence of mild mitochondrial stress markedly reduced all three T cell functions, contrasting the exposure to resveratrol, an antioxidant and sirtuin-1 activator, which specifically inhibited cytokine production and T cell proliferation, but not T cell activation. Conditions that inhibited T cell activation were associated with the downregulation of 2′,5′-oligoadenylate synthetase genes via interferon response pathways. Our findings indicate that T cell function is grossly impaired by stressors combined with nutrient deprivation, suggesting that correcting nutrient availability, metabolic stress and/or the function of T cells in these conditions will improve the efficacy of T cell-based therapies.

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Dataset ID Description Technology Samples
EGAD00001008037 Illumina HiSeq 2500 1
EGAD00001008038 Illumina HiSeq 2500 1
EGAD00001008039 Illumina HiSeq 2500 1
EGAD00001008040 Illumina HiSeq 2500 1
EGAD00001008041 Illumina HiSeq 2500 1
EGAD00001008042 Illumina HiSeq 2500 1
EGAD00001008043 Illumina HiSeq 2500 1
EGAD00001008044 Illumina HiSeq 2500 1
EGAD00001008045 Illumina HiSeq 2500 1
EGAD00001008046 Illumina HiSeq 2500 1
EGAD00001008047 Illumina HiSeq 2500 1
EGAD00001008048 Illumina HiSeq 2500 1
EGAD00001008049 Illumina HiSeq 2500 1
EGAD00001008050 Illumina HiSeq 2500 1
EGAD00001008051 Illumina HiSeq 2500 1
EGAD00001008052 Illumina HiSeq 2500 1
EGAD00001008053 Illumina HiSeq 2500 1
EGAD00001008054 Illumina HiSeq 2500 1
EGAD00001008055 Illumina HiSeq 2500 1
EGAD00001008056 Illumina HiSeq 2500 1
EGAD00001008057 Illumina HiSeq 2500 1
EGAD00001008058 Illumina HiSeq 2500 1
EGAD00001008059 Illumina HiSeq 2500 1
EGAD00001008060 Illumina HiSeq 2500 1
EGAD00001008061 Illumina HiSeq 2500 1
EGAD00001008062 Illumina HiSeq 2500 1
EGAD00001008063 Illumina HiSeq 2500 1
EGAD00001008064 Illumina HiSeq 2500 1
EGAD00001008065 Illumina HiSeq 2500 1
EGAD00001008066 Illumina HiSeq 2500 1
EGAD00001008067 Illumina HiSeq 2500 1
EGAD00001008068 Illumina HiSeq 2500 1
EGAD00001008069 Illumina HiSeq 2500 1
EGAD00001008070 Illumina HiSeq 2500 1
EGAD00001008071 Illumina HiSeq 2500 1
EGAD00001008072 Illumina HiSeq 2500 1
EGAD00001008073 Illumina HiSeq 2500 1
EGAD00001008074 Illumina HiSeq 2500 1
EGAD00001008075 Illumina HiSeq 2500 1
EGAD00001008076 Illumina HiSeq 2500 1
EGAD00001008077 Illumina HiSeq 2500 1
EGAD00001008078 Illumina HiSeq 2500 1
EGAD00001008079 Illumina HiSeq 2500 1
EGAD00001008080 Illumina HiSeq 2500 1
EGAD00001008081 Illumina HiSeq 2500 1
EGAD00001008082 Illumina HiSeq 2500 1
EGAD00001008083 Illumina HiSeq 2500 1
EGAD00001008084 Illumina HiSeq 2500 1
EGAD00001008085 Illumina HiSeq 2500 1
EGAD00001008086 Illumina HiSeq 2500 1
EGAD00001008087 Illumina HiSeq 2500 1
EGAD00001008088 Illumina HiSeq 2500 1
EGAD00001008089 Illumina HiSeq 2500 1
EGAD00001008090 Illumina HiSeq 2500 1
Publications Citations
Functional and metabolic fitness of human CD4+ T lymphocytes during metabolic stress.
Life Sci Alliance 4: 2021 e202101013
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