Gene expression by RNAseq in bronchial biopsies of asthmatics, asthma in remission and healthy subjects

The study focus was differential expression in bronchial biopsies between persistent asthma, asthma in remission and healthy controls using RNAseq.

1 Dataset 3 Publications

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Dataset ID	Description	Technology	Samples
EGAD00001005112	The study focus was differential expression in bronchial biopsies between persistent asthma, asthma in remission and healthy controls using RNAseq. There were 184 samples that passed QC. RNA samples were processed using the TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, CA), using an automated procedure in a Caliper Sciclone NGS Workstation (PerkinElmer, Waltham, MA). In this procedure, all cytoplasmic and mitochondria rRNA was removed (RiboZero Gold kit). The obtained cDNA fragment libraries were loaded in pools of multiple samples unto an Illumina HiSeq2500 sequencer using default parameters for paired-end sequencing (2 × 100 bp). Data are available as 221 pairs of FASTQ-files. Note that several samples are associated with multiple sequence runs.	Illumina HiSeq 2500	184

Publications	Citations
Gene signatures from scRNA-seq accurately quantify mast cells in biopsies in asthma. Jiang J, Faiz A, Berg M, Carpaij OA, Vermeulen CJ, Brouwer S, Hesse L, Teichmann SA, Ten Hacken NHT, Timens W, van den Berge M, Nawijn MC. Clin Exp Allergy 50: 2020 1428-1431	12
Genetic regulation of gene expression of MIF family members in lung tissue. Florez-Sampedro L, Brandsma CA, de Vries M, Timens W, Bults R, Vermeulen CJ, van den Berge M, Obeidat M, Joubert P, Nickle DC, Poelarends GJ, Faiz A, Melgert BN. Sci Rep 10: 2020 16980	5
Smoking increases expression of the SARS-CoV-2 spike protein-binding long ACE2 isoform in bronchial epithelium. Pouwels SD, van den Berge M, Vasse GF, Timens W, Brandsma CA, Aliee H, Hiemstra PS, Guryev V, Faiz A. Respir Res 24: 2023 130	1