Colorectal cancer organoid-stroma biobank cohort
In colorectal cancers (CRC) the tumor microenvironment plays a key role for prognosis and therapy efficacy. Patient-derived tumor organoids (PDTOs) show enormous potential for preclinical testing, however, purely epithelial cultures features including the ‘consensus molecular subtypes’ (CMS) are largely eradicated. To better reflect the cell type heterogeneity, we established the CRC organoid-stroma biobank of matched PDTOs and cancer-associated fibroblasts (CAFs) from 30 patients. Whole exome sequencing and transcriptome analysis in various in vitro and in vivo contexts was performed to study the influence of the TME on the CRC phenotype.
- 258 samples
- DAC: EGAC00001003246
- Technologies: Illumina HiSeq 2000, Illumina HiSeq 4000
Data access policy of organoid-stroma biobank
For access to sequencing data deposited to the European Genome-phenome Archive (EGA), application documents should be completed and returned to the Data Access Committee (DAC) of the Georg-Speyer-Haus, Institute for Tumor Biology and Experimental Therapy (email address: data-access@georg-speyer-haus.de). Applications will only be accepted electronically. Any queries regarding access procedures or completion of the forms should be sent to this address. Additional information on the data access procedure can be obtained on the EGA webpage (https://ega-archive.org/). Application requires the completion of the Data Access Agreement (including Appendix I, II and III) for each data set that must be signed by the legal entity with which you are affiliated (signed and stamped, no electronic signature). Additionally, the DAC needs a document providing proof that the applicant is working in a scientific organization (signed and stamped by a legal authority of the organization). In case of collaborative applications each institution has to submit its own application. User institutions located outside the EU/ EEA (European Economic Area) are requested to additionally enter into the Standard Contractual Clauses for the transfer of personal data to third countries. The form must be completed (including Annex A and B) and signed. Technical problems with the EGA download should be reported directly to the EGA Helpdesk. (helpdesk@ega-archive.org)
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
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