Longitudinal RNA-seq datasets from patients after abdominal surgery

This dataset consists of RNA-seq data of 126 whole-blood samples derived from patients after abdominal surgery. Total-RNA collected preoperatively and at 3-time points postoperatively (2-6, 24 and 48hrs) were analysed using RNA-sequencing. RNA was collected in PAXGene (Qiagen) tubes and extracted using the PAXGene RNA extraction kit, with a DNase step to remove contaminating DNA. Globin and ribosomal RNA were removed via a Ribo-zero kit (Illumina) and library preparation was carried out using the TruSeq Stranded Total RNA Library Prep Kit (Illumina). Next generation sequencing was performed on the NovaSeq sequencing platform (Illumina) at the Wellcome Centre for Human Genetics (WCHG) in Oxford.

252 samples
DAC: EGAC00001003318
Technology: Illumina NovaSeq 6000

Access Policy1 Study Files

Metadata

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Data access policy associated with the publication "A transcriptomic approach to understand patient susceptibility to pneumonia after abdominal surgery".

Same as for https://ega-archive.org/dacs/EGAC01000000009.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID	Study Title	Study Type
	A transcriptomic approach to understand patient susceptibility to pneumonia after abdominal surgery	Other

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