Healthy human B-lymphopoiesis RNA-Seq reference using FACS sorted bone marrow aspirates

To define a transcriptomic reference of human B lymphopoiesis, bone marrow aspirates were obtained from n=4 healthy donors (study registration DRKS00023583). After immunodensity cell separation, samples were FACS-sorted into 7 established lymphopoietic differentiation stages. RNA was extracted from 5,000-320,000 cells per differentiation stage and subjected to ultra-low-input RNA sequencing after generation of stranded sequencing libraries.

28 samples
DAC: EGAC00001003270
Technology: Illumina NovaSeq 6000

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID	Study Title	Study Type
	Healthy human B-lymphopoiesis RNA-Seq reference using FACS sorted bone marrow aspirates	RNASeq

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