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An IL-1β driven neutrophil-stromal cell axis fosters a BAFF-rich microenvironment in multiple myeloma

This depository contains data from two bulk RNA sequencing experiments: 1) Bulk RNA sequencing data of peripheral blood neutrophils from healthy donors cultured with a) human adipose-derived stromal cells (ADSC) as a model for mesenchymal stromal cells (MSC), and b) IL-1β stimulated ADSC as a model for inflammatory MSC as found in multiple myeloma (MM). 2) Bulk RNA sequencing data from ADSCs cultured a) without stimuli, b) with recombinant human IL-1β, c) with supernatant from iMSC-like cells, d) with neutrophils previously cultured with MSC, e) with neutrophils previously cultured with iMSC, f) with neutrophils previously cultured with iMSC in the presence of anti-human IL-1β or g) with neutrophils previously cultured with iMSC in the presence of an isotype control.

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Data of EMC-MM_NICHE consortium.

DATA ACCESS AGREEMENT This agreement governs the terms on which access will be granted to the genotype data generated by the EMC-MM_NICHE consortium. In signing this agreement, You are agreeing to be bound by the terms and conditions of access set out in this agreement. For the sake of clarity, the terms of access set out in this agreement apply both to the User and the User’s Institution (as defined below). User Institution and User are referred to within the agreement as “You” and “Your” shall be construed accordingly. Terms and Definitions Authorized Personnel: The individuals at the User Institution to whom EMC-MM_NICHE grants access to the Data. This includes the User, the individuals listed in Appendix II and any other individuals for whom the User Institution subsequently requests access to the Data. Details of the initial Authorized Personnel are set out in Appendix II. Data: The managed access datasets to which the User Institution has requested access. Data Producers: EMC-MM_NICHE and the collaborators listed in Appendix I responsible for the development, organization, and oversight of these Data. EMC-NICHE: Data access committee responsible for considering appeals for data belonging to study “The multiple myeloma microenvironment is defined by an inflammatory stromal cell landscape”. The DAC comprises the following members: Dr. Tom Cupedo, Dr. Mathijs A. Sanders, Remco Hoogenboezem. External Collaborator: A collaborator of the User, working for an institution other than the User Institution. Project: The project for which the User Institution has requested access to these Data. A description of the Project is set out in Appendix II. Publications: Includes, without limitation, articles published in print journals, electronic journals, reviews, books, posters and other written and verbal presentations of research. Research Participant: An individual whose data form part of these Data. Research Purposes: Shall mean research that is seeking to advance the understanding of genetics and genomics, including the treatment of disorders, and work on statistical methods that may be applied to such research. User: The principal investigator for the Project. User Institution(s): The Institution that has requested access to the Data. 1. The User Institution agrees to only use these Data for the purpose of the Project (described in Appendix II) and only for Research Purposes. The User Institution further agrees that it will only use these Data for Research Purposes which are within the limitations (if any) set out in Appendix I. 2. The User Institution agrees to preserve, at all times, the confidentiality of these Data. In particular, it undertakes not to use, or attempt to use these Data to compromise or otherwise infringe the confidentiality of information on Research Participants. Without prejudice to the generality of the foregoing, the User Institution agrees to use at least the measures set out in Appendix I to protect these Data. 3. The User Institution agrees to protect the confidentiality of Research Participants in any research papers or publications that they prepare by taking all reasonable care to limit the possibility of identification. 4. The User Institution agrees not to link or combine these Data to other information or archived data available in a way that could re-identify the Research Participants, even if access to that data has been formally granted to the User Institution or is freely available without restriction. 5. The User Institution agrees only to transfer or disclose these Data, in whole or part, or any material derived from these Data, to the Authorized Personnel. Should the User Institution wish to share these Data with an External Collaborator, the External Collaborator must complete a separate application for access to these Data. 6. The User Institution agrees that the Data Producers, and all other parties involved in the creation, funding or protection of these Data: a) make no warranty or representation, express or implied as to the accuracy, quality or comprehensiveness of these Data; b) exclude to the fullest extent permitted by law all liability for actions, claims, proceedings, demands, losses (including but not limited to loss of profit), costs, awards damages and payments made by the Recipient that may arise (whether directly or indirectly) in any way whatsoever from the Recipient’s use of these Data or from the unavailability of, or break in access to, these Data for whatever reason and; c) bear no responsibility for the further analysis or interpretation of these Data. 7. The User Institution agrees to recognizing the contribution of the Data Producers and including a proper acknowledgement in all reports or publications resulting from the use of these Data. 8. The User Institution agrees to follow the Publication Policy in Appendix III. This includes respecting the moratorium period for the Data Producers to publish the first peer-reviewed report describing and analyzing these Data. 9. The User Institution agrees not to make intellectual property claims on these Data and not to use intellectual property protection in ways that would prevent or block access to, or use of, any element of these Data, or conclusion drawn directly from these Data. 10. The User Institution can elect to perform further research that would add intellectual and resource capital to these data and decide to obtain intellectual property rights on these downstream discoveries. In this case, the User Institution agrees to implement licensing policies that will not obstruct further research and to follow the U.S. National Institutes of Health Best Practices for the Licensing of Genomic Inventions (2005) (https://www.icgc.org/files/daco/NIH_BestPracticesLicensingGenomicInventions_2005_en.pdf ) in conformity with the Organization for Economic Co-operation and Development Guidelines for the Licensing of the Genetic Inventions (2006) (http://www.oecd.org/science/biotech/36198812.pdf ). 11. The User Institution agrees to destroy/discard the Data held, once it is no longer used for the Project, unless obliged to retain the data for archival purposes in conformity with audit or legal requirements. 12. The User Institution will notify EMC-MM_NICHE within 30 days of any changes or departures of Authorized Personnel. 13. The User Institution will notify EMC-MM_NICHE prior to any significant changes to the protocol for the Project. 14. The User Institution will notify EMC-MM_NICHE as soon as it becomes aware of a breach of the terms or conditions of this agreement. 15. EMC-MM_NICHE may terminate this agreement by written notice to the User Institution. If this agreement terminates for any reason, the User Institution will be required to destroy any Data held, including copies and backup copies. This clause does not prevent the User Institution from retaining these data for archival purpose in conformity with audit or legal requirements. 16. The User Institution accepts that it may be necessary for the Data Producers to alter the terms of this agreement from time to time. As an example, this may include specific provisions relating to the Data required by Data Producers other than EMC-MM_NICHE. In the event that changes are required, the Data Producers or their appointed agent will contact the User Institution to inform it of the changes and the User Institution may elect to accept the changes or terminate the agreement. 17. If requested, the User Institution will allow data security and management documentation to be inspected to verify that it is complying with the terms of this agreement. 18. The User Institution agrees to distribute a copy of these terms to the Authorized Personnel. The User Institution will procure that the Authorized Personnel comply with the terms of this agreement. 19. This agreement (and any dispute, controversy, proceedings or claim of whatever nature arising out of this agreement or its formation) shall be construed, interpreted and governed by the laws of the Netherlands and shall be subject to the exclusive jurisdiction of the Dutch courts.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001007038 Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00007953986 fastq.gz 3.4 GB
EGAF00007953987 fastq.gz 3.6 GB
EGAF00007953988 fastq.gz 2.7 GB
EGAF00007953989 fastq.gz 2.8 GB
EGAF00007953990 fastq.gz 3.4 GB
EGAF00007953991 fastq.gz 3.9 GB
EGAF00007953992 fastq.gz 5.9 GB
EGAF00007953993 fastq.gz 6.2 GB
EGAF00007953994 fastq.gz 680.9 MB
EGAF00007953995 fastq.gz 733.9 MB
EGAF00007953996 fastq.gz 9.0 GB
EGAF00007953997 fastq.gz 9.5 GB
EGAF00007953998 fastq.gz 5.3 GB
EGAF00007953999 fastq.gz 5.6 GB
EGAF00007954000 fastq.gz 2.6 GB
EGAF00007954001 fastq.gz 2.8 GB
EGAF00007954002 fastq.gz 2.1 GB
EGAF00007954003 fastq.gz 2.2 GB
EGAF00007954004 fastq.gz 1.8 GB
EGAF00007954005 fastq.gz 1.9 GB
EGAF00007954006 fastq.gz 2.6 GB
EGAF00007954007 fastq.gz 2.7 GB
EGAF00007954008 fastq.gz 1.9 GB
EGAF00007954009 fastq.gz 2.0 GB
EGAF00007954010 fastq.gz 2.6 GB
EGAF00007954011 fastq.gz 2.8 GB
EGAF00007954012 fastq.gz 1.8 GB
EGAF00007954013 fastq.gz 2.0 GB
EGAF00007954014 fastq.gz 3.7 GB
EGAF00007954015 fastq.gz 3.8 GB
EGAF00007954016 fastq.gz 3.1 GB
EGAF00007954017 fastq.gz 3.3 GB
EGAF00007954018 fastq.gz 13.2 GB
EGAF00007954019 fastq.gz 13.9 GB
EGAF00007954020 fastq.gz 2.0 GB
EGAF00007954021 fastq.gz 2.1 GB
EGAF00007954022 fastq.gz 1.8 GB
EGAF00007954023 fastq.gz 1.9 GB
EGAF00007954024 fastq.gz 1.7 GB
EGAF00007954025 fastq.gz 1.8 GB
EGAF00007954026 fastq.gz 8.4 GB
EGAF00007954027 fastq.gz 9.2 GB
EGAF00007954028 fastq.gz 1.7 GB
EGAF00007954029 fastq.gz 1.8 GB
EGAF00007954030 fastq.gz 1.9 GB
EGAF00007954031 fastq.gz 2.0 GB
EGAF00007954032 fastq.gz 2.5 GB
EGAF00007954033 fastq.gz 2.7 GB
EGAF00007954034 fastq.gz 3.9 GB
EGAF00007954035 fastq.gz 4.1 GB
EGAF00007954036 fastq.gz 2.7 GB
EGAF00007954037 fastq.gz 2.8 GB
EGAF00007954038 fastq.gz 2.0 GB
EGAF00007954039 fastq.gz 2.1 GB
EGAF00007954040 fastq.gz 2.1 GB
EGAF00007954041 fastq.gz 2.3 GB
EGAF00007954042 fastq.gz 367.7 MB
EGAF00007954043 fastq.gz 389.3 MB
EGAF00007954044 fastq.gz 2.6 GB
EGAF00007954045 fastq.gz 2.7 GB
EGAF00007954046 fastq.gz 2.2 GB
EGAF00007954047 fastq.gz 2.3 GB
EGAF00007954048 fastq.gz 8.2 GB
EGAF00007954049 fastq.gz 8.5 GB
EGAF00007954050 fastq.gz 2.2 GB
EGAF00007954051 fastq.gz 2.2 GB
EGAF00007954052 fastq.gz 2.1 GB
EGAF00007954053 fastq.gz 2.2 GB
EGAF00007954054 fastq.gz 2.2 GB
EGAF00007954055 fastq.gz 2.3 GB
EGAF00007954056 fastq.gz 2.0 GB
EGAF00007954057 fastq.gz 2.0 GB
EGAF00007954058 fastq.gz 1.9 GB
EGAF00007954059 fastq.gz 2.0 GB
EGAF00007954060 fastq.gz 1.9 GB
EGAF00007954061 fastq.gz 2.0 GB
EGAF00007954062 fastq.gz 2.1 GB
EGAF00007954063 fastq.gz 2.2 GB
EGAF00007954064 fastq.gz 2.0 GB
EGAF00007954065 fastq.gz 2.1 GB
EGAF00007954066 fastq.gz 1.9 GB
EGAF00007954067 fastq.gz 2.0 GB
82 Files (263.5 GB)