RNA-sequencing of ex vivo exhausted human antigen-specific T cells
Tumor-specific T cells are frequently exhausted by chronic antigenic stimulation. To explore new pathways for reinvigoration of anti-tumor immune functions, we developed a human ex vivo exhaustion model by repetitive antigenic stimulation of primary CD8 T cells. This results in T cells that resemble patient-derived T cells in tumors on a phenotypic and transcriptional level. Four human healhy donor CD8+ T cells were isolated, transduced with an NY-ESO-1 TCR lentivirus construct, stimulated in four different conditions (Trested, Ttumor, Tex, Teff) with T2 tumor cells and specific peptides over 12 days. Cells were then sorted for TCR Vbeta 13.1+ (NY-ESO-1 TCR) CD8+ CD3+ CD56- CD4- DAPI- cells. RNA-seq TruSeq libraries were generated from polyA-enriched mRNA isolated from the samples, and sequenced in paired-end mode (2x51bp) on 2 lanes of an Illumina NovaSeq 6000 flow-cell. FASTQ sequence files were generated with the Illumina RTA version 3.4.4 and Base-calling Version bcl2fastq-2.20.0.422.
- 16 samples
- DAC: EGAC00001002981
- Technology: Illumina NovaSeq 6000
- HMB DUO:0000006 (version: 2021-02-23)health or medical or biomedical researchThis data use permission indicates that use is allowed for health/medical/biomedical purposes; does not include the study of population origins or ancestry.
- MOR DUO:0000024 (version: 2021-02-23)publication moratoriumThis data use modifier indicates that requestor agrees not to publish results of studies until a specific date.
not available - do not use
not available - do not use
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| Other |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF00007774187 | 1669256126129 | 655.3 MB | ||
| EGAF00007774188 | 1669256126129 | 690.6 MB | ||
| EGAF00007774189 | 1669256126129 | 648.1 MB | ||
| EGAF00007774190 | 1669256126129 | 682.7 MB | ||
| EGAF00007774191 | 1669256126129 | 638.1 MB | ||
| EGAF00007774192 | 1669256126129 | 664.5 MB | ||
| EGAF00007774193 | 1669256126129 | 629.9 MB | ||
| EGAF00007774194 | 1669256126129 | 655.8 MB | ||
| EGAF00007774195 | 1669256126129 | 875.5 MB | ||
| EGAF00007774196 | 1669256126129 | 914.6 MB | ||
| EGAF00007774197 | 1669256126129 | 864.6 MB | ||
| EGAF00007774198 | 1669256126129 | 902.5 MB | ||
| EGAF00007774199 | 1669256126129 | 771.2 MB | ||
| EGAF00007774200 | 1669256126129 | 805.2 MB | ||
| EGAF00007774201 | 1669256126129 | 760.9 MB | ||
| EGAF00007774202 | 1669256126129 | 794.1 MB | ||
| EGAF00007774203 | 1669256126129 | 817.0 MB | ||
| EGAF00007774204 | 1669256126129 | 852.2 MB | ||
| EGAF00007774205 | 1669256126129 | 808.1 MB | ||
| EGAF00007774206 | 1669256126129 | 842.5 MB | ||
| EGAF00007774207 | 1669255261196 | 788.0 MB | ||
| EGAF00007774208 | 1669256126129 | 818.2 MB | ||
| EGAF00007774209 | 1669256126129 | 777.9 MB | ||
| EGAF00007774210 | 1669255261196 | 807.5 MB | ||
| EGAF00007774211 | 1669256126129 | 761.8 MB | ||
| EGAF00007774212 | 1669255261196 | 801.2 MB | ||
| EGAF00007774213 | 1669256126129 | 753.5 MB | ||
| EGAF00007774214 | 1669255261196 | 792.1 MB | ||
| EGAF00007774215 | 1669257028306 | 779.6 MB | ||
| EGAF00007774216 | 1669256126129 | 811.4 MB | ||
| EGAF00007774217 | 1669255261196 | 769.3 MB | ||
| EGAF00007774218 | 1669256126129 | 800.3 MB | ||
| EGAF00007774219 | 1669255261196 | 662.1 MB | ||
| EGAF00007774220 | 1669255261196 | 704.0 MB | ||
| EGAF00007774221 | 1669256126129 | 655.5 MB | ||
| EGAF00007774222 | 1669256126129 | 696.5 MB | ||
| EGAF00007774223 | 1669256126129 | 675.8 MB | ||
| EGAF00007774224 | 1669255261196 | 706.5 MB | ||
| EGAF00007774225 | 1669256126129 | 667.2 MB | ||
| EGAF00007774226 | 1669256126129 | 697.2 MB | ||
| EGAF00007774227 | 1669256126129 | 603.5 MB | ||
| EGAF00007774228 | 1669256126129 | 634.7 MB | ||
| EGAF00007774229 | 1669256126129 | 596.2 MB | ||
| EGAF00007774230 | 1669255261196 | 626.6 MB | ||
| EGAF00007774231 | 1669256126129 | 606.0 MB | ||
| EGAF00007774232 | 1669255261196 | 637.9 MB | ||
| EGAF00007774233 | 1669256126129 | 598.1 MB | ||
| EGAF00007774234 | 1669255261196 | 629.1 MB | ||
| EGAF00007774235 | 1669255261196 | 681.3 MB | ||
| EGAF00007774236 | 1669255261196 | 713.8 MB | ||
| EGAF00007774237 | 1669255261196 | 671.7 MB | ||
| EGAF00007774238 | 1669256126129 | 703.4 MB | ||
| EGAF00007774239 | 1669255261196 | 769.1 MB | ||
| EGAF00007774240 | 1669256126129 | 802.4 MB | ||
| EGAF00007774241 | 1669255261196 | 759.7 MB | ||
| EGAF00007774242 | 1669255261196 | 792.1 MB | ||
| EGAF00007774243 | 1669263321225 | 793.0 MB | ||
| EGAF00007774244 | 1669255261196 | 830.2 MB | ||
| EGAF00007774245 | 1669256126129 | 785.3 MB | ||
| EGAF00007774246 | 1669255261196 | 821.8 MB | ||
| EGAF00007774247 | 1669255261196 | 699.0 MB | ||
| EGAF00007774248 | 1669255261196 | 724.5 MB | ||
| EGAF00007774249 | 1669255261196 | 689.6 MB | ||
| EGAF00007774250 | 1669255261196 | 714.6 MB | ||
| 64 Files (47.1 GB) | ||||