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minION fastq files of 10 different tumor samples from the Master program (H021)

Nanopore RNA Sequencing was done for 10 tumor samples. Direct cDNA sequencing was performed using the SQK-DCS109 kit (Oxford Nanopore Technologies). For analysis of a single sample on a MinION flow cell (version R9.4.1), 5 μg RNA was used as input. For multiplexing on a MinION flow cell, 2.5 μg RNA per sample was used as input, and the native barcoding expansion kit EXP-NBD104 was employed in conjunction with SQK-DCS109. After reverse transcription with Maxima H Minus Reverse Transcriptase (Thermo Scientific), second-strand synthesis was performed using the 2x LongAmp Taq Master Mix (New England Biolabs). The resulting double-stranded cDNA was subjected to end-repair and dA-tailing using the NEBNext Ultra End Repair/dA-Tailing Module (New England Biolabs). For multiplexed libraries, this step was followed by barcode ligation and library pooling. Next, libraries were quantified with a Qubit Fluorometer 3.0 (Life Technologies). Finally, sequencing adapters were added to the library preparations and ligated with Blunt/TA Ligase Master Mix (New England Biolabs), followed by further quality control using a Qubit. Samples ACC1 and ACC2 were analyzed on individual MinION flow cells, while the remaining eight samples were sequenced as multiplexed libraries on two MinION flow cells by pooling four samples for each run. Five ACC samples were also analyzed individually on Flongle flow cells

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Data Transfer Agreement for the German Cancer Research Center

To request access to the dataset please start a Data Access Request via the EGA DAC Portal. You will receive a HIPO DACO Info Form by email to collect information about the request, which has to be filled completely and signed by your PI. Your request will be evaluated by the responsible Data Access Committee (DAC) and our Data protection department (and innovation management if required). As soon as a positive decision is made, you will be provided with a data transfer agreement (DTA). After the DTA has been fully signed, access to the requested data is granted. To ensure that the process can be completed as quickly as possible, please note the following points: 1. The request in the EGA portal must be made by the responsible PI. 2. The information in the EGA portal must match the information on the HIPO DACO Info Form. In particular, the email address and the requested data sets must match. It is also important that all requested data sets are also listed on the HIPO DACO Info Form. For-profit companies are charged a fee per request.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001006317 Cancer Genomics

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00006210366 fastq.gz 2.7 GB
EGAF00006210367 fastq.gz 1.9 GB
EGAF00006210368 fastq.gz 30.1 MB
EGAF00006210369 fastq.gz 62.8 MB
EGAF00006210370 fastq.gz 8.3 MB
EGAF00006210371 fastq.gz 13.4 MB
EGAF00006210372 fastq.gz 6.0 MB
EGAF00006210373 fastq.gz 215.7 MB
EGAF00006210374 fastq.gz 474.0 MB
EGAF00006210375 fastq.gz 186.6 MB
EGAF00006210376 fastq.gz 624.8 MB
EGAF00006210377 fastq.gz 525.3 MB
EGAF00006210378 fastq.gz 479.1 MB
EGAF00006210379 fastq.gz 505.0 MB
EGAF00006210380 fastq.gz 570.8 MB
15 Files (8.3 GB)