Blood neutrophils in COPD derive from activated progenitors in the bone marrow
Chronic obstructive pulmonary disease (COPD) is a major respiratory disease characterized by small airway inflammation, emphysema and severe breathing difficulties. Low-grade systemic inflammation is an established hallmark of severe disease, however, the molecular changes in peripheral immune cells remain far from understood. We combined multi-color flow cytometry with single-cell RNA sequencing and showed that blood neutrophil numbers are significantly increased in COPD and they are a heterogeneous population. A transcriptomic state that expressed interferon response genes correlated with alveolar damage and acute exacerbations. Furthermore, bronchoalveolar neutrophils expressed gene signatures corresponding to certain blood neutrophil states. Last, our data in a murine model of cigarette smoke exposure demonstrated that bone marrow neutrophil progenitors are expanded in smoke-treated animals and display signs of immune activation. Our study provides evidence that COPD systemic inflammation may derive from an activated haematopoietic precursor compartment.
- 25 samples
- DAC: EGAC00001002673
- Technology: NextSeq 500
Chronic obstructive pulmonary disease (COPD) is a major respiratory disease characterized by small airway inflammation, emphysema and severe breathing difficulties. Low-grade systemic inflammation is an established hallmark of severe disease, however, the molecular changes in peripheral immune cells remain far from understood. We combined multi-color flow cytometry with single-cell RNA sequencing and showed that blood neutrophil numbers are significantly increased in COPD and they are a heterogeneous population. A transcriptomic state that expressed interferon response genes correlated with alveolar damage and acute exacerbations. Furthermore, bronchoalveolar neutrophils expressed gene signatures corresponding to certain blood neutrophil states. Last, our data in a murine model of cigarette smoke exposure demonstrated that bone marrow neutrophil progenitors are expanded in smoke-treated animals and display signs of immune activation. Our study provides evidence that COPD systemic inflammation may derive from an activated haematopoietic precursor compartment.
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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS00001006281 | Other |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF00006173277 | fastq.gz | 2.0 GB | ||
| EGAF00006173278 | fastq.gz | 851.9 MB | ||
| EGAF00006173279 | fastq.gz | 1.8 GB | ||
| EGAF00006173280 | fastq.gz | 714.8 MB | ||
| EGAF00006173281 | fastq.gz | 706.4 MB | ||
| EGAF00006173282 | fastq.gz | 1.3 GB | ||
| EGAF00006173283 | fastq.gz | 545.1 MB | ||
| EGAF00006173284 | fastq.gz | 740.6 MB | ||
| EGAF00006173285 | fastq.gz | 1.1 GB | ||
| EGAF00006173286 | fastq.gz | 533.4 MB | ||
| EGAF00006173287 | fastq.gz | 1.3 GB | ||
| EGAF00006173288 | fastq.gz | 481.7 MB | ||
| EGAF00006173289 | fastq.gz | 810.7 MB | ||
| EGAF00006173290 | fastq.gz | 435.7 MB | ||
| EGAF00006173291 | fastq.gz | 533.3 MB | ||
| EGAF00006173292 | fastq.gz | 1.2 GB | ||
| EGAF00006173293 | fastq.gz | 391.2 MB | ||
| EGAF00006173294 | fastq.gz | 933.2 MB | ||
| EGAF00006173295 | fastq.gz | 705.8 MB | ||
| EGAF00006173296 | fastq.gz | 1.1 GB | ||
| EGAF00006173297 | fastq.gz | 1.4 GB | ||
| EGAF00006173298 | fastq.gz | 1.1 GB | ||
| EGAF00006173299 | fastq.gz | 1.3 GB | ||
| EGAF00006173300 | fastq.gz | 782.4 MB | ||
| EGAF00006173301 | fastq.gz | 542.9 MB | ||
| 25 Files (23.3 GB) | ||||