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Single cell sequencing of human normal luminal cells

The dataset includes 12 paired FASTQ files (6 samples) with single cell transcriptome sequencing data of normal breast luminal cells from ducts and TDLUs derived from reduction mammoplasties from three patients. Chromium Single Cell 3’ Reagent Kit v2 or v3 (10x Genomics) were used for processing of cells, whereafter sequencing was performed using the Illumina® NextSeq500/550 High Output Kit v2. Cell Ranger was used for generating FASTQ files and files from different lanes were concatenated prior to uploading the data to EGA. "R1" files include the feature barcodes and UMIs, while "R2" files include the reads.

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Data access committee for studying human normal breast cells from reduction mammoplasty tissue

This Data Access Committee (DAC) will evaluate any request provided by a requestor, and grant access to specific datasets to those who fulfill the criteria for data access within EGA. The Data Access Committee (DAC) will aim to respond to all initial requests within 2 weeks. The committee will check that requestors data/EGAD number is consistent with the current data submission (that requestor has contacted the correct DAC). Requestor has to sign up to the terms within the DAA in order to validate that the data are used within terms of consent, and document this to the committee. Requestor has to provide a valid Institutional email address as well as a valid affiliation to an institutional organization. Requestor has to document to be “appropriately qualified/bona fide” for use of the data.

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00005783348 1643124240161 2.8 GB
EGAF00005783349 1643123400831 5.1 GB
EGAF00005783350 1643123640160 2.7 GB
EGAF00005783351 1643139480092 8.2 GB
EGAF00005783352 1643123100210 1.5 GB
EGAF00005783353 1643123220180 2.7 GB
EGAF00005783354 1643123760076 3.2 GB
EGAF00005783355 1643124660168 9.1 GB
EGAF00005783356 1643123220180 2.9 GB
EGAF00005783357 1643140320109 8.8 GB
EGAF00005783358 1643123220180 3.0 GB
EGAF00005783359 1643124060049 8.6 GB
12 Files (58.5 GB)