RNA sequencing data of in vitro differentiated megakaryocyte cells transduced with E527K and WT SRC
RNA sequencing data of in vitro differentiated megakaryocyte cells transduced with E527K and WT SRC. CD34+ hematopoietic stem cells (HSC) were isolated from healthy controls before transduction with WT-SRC and E527K-SRC lentiviral vectors in triplicate and differentiation to MK. Three replicates each of two pools were generated for both WT and E527K SRC transduced cells, resulting in 3 WT pool 1 samples, 3 WT pool 2 samples, 3 E527K pool 1 samples and 3 E527K pool 2 samples for a total of 12 samples. RNA was extracted and sequenced with following parameters: Platform: Illumina HiSeq4000, Library Prep Kit: TruSeq stranded mRNA, Sequencing Kit: Illumina HiSeq4000 100 cycles (76-8-8-7), Fragments: single end / fr-firststrand.
- 12 samples
- DAC: EGAC00001002416
- Technology: Illumina HiSeq 4000
DAA terms and conditions for RNA sequencing data
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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
Study ID | Study Title | Study Type |
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Other |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
ID | File Type | Size | Located in | |
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EGAF00005719883 | 1637341561189 | 1.6 GB | ||
EGAF00005719884 | 1637341561189 | 1.7 GB | ||
EGAF00005719885 | 1637341561189 | 1.5 GB | ||
EGAF00005719886 | 1637341561189 | 1.7 GB | ||
EGAF00005719887 | 1637341561189 | 1.5 GB | ||
EGAF00005719888 | 1637341561189 | 1.7 GB | ||
EGAF00005719889 | 1637341620041 | 1.8 GB | ||
EGAF00005719890 | 1637341561188 | 1.8 GB | ||
EGAF00005719891 | 1637341561189 | 1.7 GB | ||
EGAF00005719892 | 1637341561189 | 1.6 GB | ||
EGAF00005719893 | 1637341561189 | 1.7 GB | ||
EGAF00005719894 | 1637341561189 | 1.6 GB | ||
12 Files (19.9 GB) |