Metabolic context regulates the competitive fitness of oncogenic PIK3CA mutant clones in the normal esophagus
Activating mutations in PIK3CA generate large clones in the aging human esophagus. Here we investigate the underlying cellular mechanisms regulating their expansion by lineage tracing. Expression of an activating heterozygous Pik3caH1047R mutation in single progenitor cells of the mouse esophagus tilts cell fate towards proliferation, generating mutant clones that outcompete their wild type neighbours. The mutation leads to increased aerobic glycolysis through the activation of Hif1α transcriptional targets. In vitro and in vivo interventions that level out differences in activation of the PI3K/HIF1α/aerobic glycolysis axis between wild type and Pik3caH1047R cells attenuate the competitive advantage of the mutants. In contrast, metabolic conditions that alter Insulin/PI3K signalling, such as type-1 diabetes or diet-induced insulin resistance, further increase Pik3caH1047R mutant competitiveness in mice. Consistently, the density of activating PIK3CA mutations in human esophagus is increased in overweight individuals. We conclude that the metabolic environment influences the mutational landscape of normal epithelia. Clinically feasible interventions that even out signalling imbalances between wild type and mutant cells may therefore limit the expansion of oncogenic mutants in normal tissues.
- 157 samples
- DAC: EGAC00001000000
- Technology: Illumina HiSeq 2500
- PUB DUO:0000019 (version: 2021-02-23)publication requiredThis data use modifier indicates that requestor agrees to make results of studies using the data available to the larger scientific community.
- US DUO:0000026 (version: 2021-02-23)user specific restrictionThis data use modifier indicates that use is limited to use by approved users.
- IS DUO:0000028 (version: 2021-02-23)institution specific restrictionThis data use modifier indicates that use is limited to use within an approved institution.
- GRU DUO:0000042 (version: 2021-02-23)general research useThis data use permission indicates that use is allowed for general research use for any research purpose.
Wellcome Trust Sanger Institute Cancer Genome Group Data Sharing Policy
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
Study ID | Study Title | Study Type |
---|---|---|
EGAS00001001933 | Other |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
ID | File Type | Size | Located in | |
---|---|---|---|---|
EGAF00005563767 | bam | 8.4 MB | ||
EGAF00005563768 | bam | 9.0 MB | ||
EGAF00005563769 | bam | 8.8 MB | ||
EGAF00005563770 | bam | 9.6 MB | ||
EGAF00005563771 | bam | 9.4 MB | ||
EGAF00005563772 | bam | 9.7 MB | ||
EGAF00005563773 | bam | 9.4 MB | ||
EGAF00005563774 | bam | 9.9 MB | ||
EGAF00005563775 | bam | 9.6 MB | ||
EGAF00005563776 | bam | 8.8 MB | ||
EGAF00005563777 | bam | 9.8 MB | ||
EGAF00005563778 | bam | 9.0 MB | ||
EGAF00005563779 | bam | 9.0 MB | ||
EGAF00005563780 | bam | 9.8 MB | ||
EGAF00005563781 | bam | 9.3 MB | ||
EGAF00005563782 | bam | 9.9 MB | ||
EGAF00005563783 | bam | 4.2 MB | ||
EGAF00005563784 | bam | 4.3 MB | ||
EGAF00005563785 | bam | 4.0 MB | ||
EGAF00005563786 | bam | 4.3 MB | ||
EGAF00005563787 | bam | 3.9 MB | ||
EGAF00005563788 | bam | 4.0 MB | ||
EGAF00005563789 | bam | 4.1 MB | ||
EGAF00005563790 | bam | 4.2 MB | ||
EGAF00005563791 | bam | 3.9 MB | ||
EGAF00005563792 | bam | 3.8 MB | ||
EGAF00005563793 | bam | 4.0 MB | ||
EGAF00005563794 | bam | 4.3 MB | ||
EGAF00005563795 | bam | 3.9 MB | ||
EGAF00005563796 | bam | 4.2 MB | ||
EGAF00005563797 | bam | 3.9 MB | ||
EGAF00005563798 | bam | 4.1 MB | ||
EGAF00005563799 | bam | 9.2 MB | ||
EGAF00005563800 | bam | 7.4 MB | ||
EGAF00005563801 | bam | 8.4 MB | ||
EGAF00005563802 | bam | 9.7 MB | ||
EGAF00005563803 | bam | 9.2 MB | ||
EGAF00005563804 | bam | 9.0 MB | ||
EGAF00005563805 | bam | 9.3 MB | ||
EGAF00005563806 | bam | 10.4 MB | ||
EGAF00005563807 | bam | 9.0 MB | ||
EGAF00005563808 | bam | 8.7 MB | ||
EGAF00005563809 | bam | 8.1 MB | ||
EGAF00005563810 | bam | 9.3 MB | ||
EGAF00005563811 | bam | 8.8 MB | ||
EGAF00005563812 | bam | 8.1 MB | ||
EGAF00005563813 | bam | 8.5 MB | ||
EGAF00005563814 | bam | 9.2 MB | ||
EGAF00005563815 | bam | 11.4 MB | ||
EGAF00005563816 | bam | 11.6 MB | ||
EGAF00005563817 | bam | 11.1 MB | ||
EGAF00005563818 | bam | 11.6 MB | ||
EGAF00005563819 | bam | 11.9 MB | ||
EGAF00005563820 | bam | 11.9 MB | ||
EGAF00005563821 | bam | 11.5 MB | ||
EGAF00005563822 | bam | 11.6 MB | ||
EGAF00005563823 | bam | 12.3 MB | ||
EGAF00005563824 | bam | 12.0 MB | ||
EGAF00005563825 | bam | 12.2 MB | ||
EGAF00005563826 | bam | 11.6 MB | ||
EGAF00005563827 | bam | 12.0 MB | ||
EGAF00005563828 | bam | 12.8 MB | ||
EGAF00005563829 | bam | 11.1 MB | ||
EGAF00005563830 | bam | 11.2 MB | ||
EGAF00005563831 | bam | 6.2 MB | ||
EGAF00005563832 | bam | 6.3 MB | ||
EGAF00005563833 | bam | 6.4 MB | ||
EGAF00005563834 | bam | 6.8 MB | ||
EGAF00005563835 | bam | 7.1 MB | ||
EGAF00005563836 | bam | 6.6 MB | ||
EGAF00005563837 | bam | 6.7 MB | ||
EGAF00005563838 | bam | 6.8 MB | ||
EGAF00005563839 | bam | 6.6 MB | ||
EGAF00005563840 | bam | 6.6 MB | ||
EGAF00005563841 | bam | 6.8 MB | ||
EGAF00005563842 | bam | 6.0 MB | ||
EGAF00005563843 | bam | 6.7 MB | ||
EGAF00005563844 | bam | 6.5 MB | ||
EGAF00005563845 | bam | 6.3 MB | ||
EGAF00005563846 | bam | 6.0 MB | ||
EGAF00005563847 | bam | 5.2 MB | ||
EGAF00005563848 | bam | 5.4 MB | ||
EGAF00005563849 | bam | 5.7 MB | ||
EGAF00005563850 | bam | 5.5 MB | ||
EGAF00005563851 | bam | 5.2 MB | ||
EGAF00005563852 | bam | 6.0 MB | ||
EGAF00005563853 | bam | 5.6 MB | ||
EGAF00005563854 | bam | 6.2 MB | ||
EGAF00005563855 | bam | 7.0 MB | ||
EGAF00005563856 | bam | 6.7 MB | ||
EGAF00005563857 | bam | 7.5 MB | ||
EGAF00005563858 | bam | 5.8 MB | ||
EGAF00005563859 | bam | 7.2 MB | ||
EGAF00005563860 | bam | 6.7 MB | ||
EGAF00005563861 | bam | 6.7 MB | ||
EGAF00005563862 | bam | 7.0 MB | ||
EGAF00005563863 | bam | 6.8 MB | ||
EGAF00005563864 | bam | 6.4 MB | ||
EGAF00005563865 | bam | 6.8 MB | ||
EGAF00005563866 | bam | 6.8 MB | ||
EGAF00005563867 | bam | 6.9 MB | ||
EGAF00005563868 | bam | 6.0 MB | ||
EGAF00005563869 | bam | 5.7 MB | ||
EGAF00005563870 | bam | 6.4 MB | ||
EGAF00005563871 | bam | 6.7 MB | ||
EGAF00005563872 | bam | 6.9 MB | ||
EGAF00005563873 | bam | 6.8 MB | ||
EGAF00005563874 | bam | 6.5 MB | ||
EGAF00005563875 | bam | 6.5 MB | ||
EGAF00005563876 | bam | 6.4 MB | ||
EGAF00005563877 | bam | 7.7 MB | ||
EGAF00005563878 | bam | 7.0 MB | ||
EGAF00005563879 | bam | 7.8 MB | ||
EGAF00005563880 | bam | 6.8 MB | ||
EGAF00005563881 | bam | 6.9 MB | ||
EGAF00005563882 | bam | 7.2 MB | ||
EGAF00005563883 | bam | 6.7 MB | ||
EGAF00005563884 | bam | 6.8 MB | ||
EGAF00005563885 | bam | 6.4 MB | ||
EGAF00005563886 | bam | 6.6 MB | ||
EGAF00005563887 | bam | 7.5 MB | ||
EGAF00005563888 | bam | 7.2 MB | ||
EGAF00005563889 | bam | 6.4 MB | ||
EGAF00005563890 | bam | 7.5 MB | ||
EGAF00005563891 | bam | 6.0 MB | ||
EGAF00005563892 | bam | 6.4 MB | ||
EGAF00005563893 | bam | 7.3 MB | ||
EGAF00005563894 | bam | 7.6 MB | ||
EGAF00005563895 | bam | 7.1 MB | ||
EGAF00005563896 | bam | 7.2 MB | ||
EGAF00005563897 | bam | 7.6 MB | ||
EGAF00005563898 | bam | 6.9 MB | ||
EGAF00005563899 | bam | 6.6 MB | ||
EGAF00005563900 | bam | 7.2 MB | ||
EGAF00005563901 | bam | 7.4 MB | ||
EGAF00005563902 | bam | 6.6 MB | ||
EGAF00005563903 | bam | 6.1 MB | ||
EGAF00005563904 | bam | 6.5 MB | ||
EGAF00005563905 | bam | 6.3 MB | ||
EGAF00005563906 | bam | 6.7 MB | ||
EGAF00005563907 | bam | 6.6 MB | ||
EGAF00005563908 | bam | 6.4 MB | ||
EGAF00005563909 | bam | 7.1 MB | ||
EGAF00005563910 | bam | 6.4 MB | ||
EGAF00005563911 | bam | 6.5 MB | ||
EGAF00005563912 | bam | 6.8 MB | ||
EGAF00005563913 | bam | 6.6 MB | ||
EGAF00005563914 | bam | 6.4 MB | ||
EGAF00005563915 | bam | 6.7 MB | ||
EGAF00005563916 | bam | 6.6 MB | ||
EGAF00005563917 | bam | 6.6 MB | ||
EGAF00005563918 | bam | 5.6 MB | ||
EGAF00005563919 | bam | 5.5 MB | ||
EGAF00005563920 | bam | 5.5 MB | ||
EGAF00005563921 | bam | 5.6 MB | ||
EGAF00005563922 | bam | 5.5 MB | ||
EGAF00005563923 | bam | 5.3 MB | ||
EGAF00005563924 | bam | 5.4 MB | ||
EGAF00005563925 | bam | 5.7 MB | ||
EGAF00005563926 | bam | 6.1 MB | ||
EGAF00005563927 | bam | 9.2 MB | ||
EGAF00005563928 | bam | 9.5 MB | ||
EGAF00005563929 | bam | 9.4 MB | ||
EGAF00005563930 | bam | 10.5 MB | ||
EGAF00005563931 | bam | 11.1 MB | ||
EGAF00005563932 | bam | 13.4 MB | ||
EGAF00005563933 | bam | 10.4 MB | ||
EGAF00005563934 | bam | 9.2 MB | ||
EGAF00005563935 | bam | 9.3 MB | ||
EGAF00005563936 | bam | 8.9 MB | ||
EGAF00005563937 | bam | 10.3 MB | ||
EGAF00005563938 | bam | 9.6 MB | ||
EGAF00005563939 | bam | 10.4 MB | ||
EGAF00005563940 | bam | 10.4 MB | ||
EGAF00005563941 | bam | 10.7 MB | ||
EGAF00005563942 | bam | 12.9 MB | ||
EGAF00005563943 | bam | 11.6 MB | ||
EGAF00005563944 | bam | 10.4 MB | ||
EGAF00005563945 | bam | 9.8 MB | ||
EGAF00005563946 | bam | 9.5 MB | ||
EGAF00005563947 | bam | 10.5 MB | ||
EGAF00005563948 | bam | 10.8 MB | ||
EGAF00005563949 | bam | 11.4 MB | ||
EGAF00005563950 | bam | 9.9 MB | ||
EGAF00005563951 | bam | 9.6 MB | ||
EGAF00005563952 | bam | 8.6 MB | ||
EGAF00005563953 | bam | 10.2 MB | ||
EGAF00005563954 | bam | 9.8 MB | ||
EGAF00005563955 | bam | 10.2 MB | ||
EGAF00005563956 | bam | 10.0 MB | ||
EGAF00005563957 | bam | 9.8 MB | ||
EGAF00005563958 | bam | 12.0 MB | ||
EGAF00005563959 | bam | 11.8 MB | ||
EGAF00005563960 | bam | 10.1 MB | ||
EGAF00005563961 | bam | 9.8 MB | ||
EGAF00005563962 | bam | 10.1 MB | ||
EGAF00005563963 | bam | 10.9 MB | ||
EGAF00005563964 | bam | 10.2 MB | ||
EGAF00005563965 | bam | 9.9 MB | ||
EGAF00005563966 | bam | 10.5 MB | ||
EGAF00005563967 | bam | 9.7 MB | ||
EGAF00005563968 | bam | 9.7 MB | ||
EGAF00005563969 | bam | 9.4 MB | ||
EGAF00005563970 | bam | 9.8 MB | ||
EGAF00005563971 | bam | 10.0 MB | ||
EGAF00005563972 | bam | 9.7 MB | ||
EGAF00005563973 | bam | 10.2 MB | ||
EGAF00005563974 | bam | 10.6 MB | ||
EGAF00005563975 | bam | 9.7 MB | ||
EGAF00005563976 | bam | 9.5 MB | ||
EGAF00005563977 | bam | 9.8 MB | ||
EGAF00005563978 | bam | 7.7 MB | ||
EGAF00005563979 | bam | 9.9 MB | ||
EGAF00005563980 | bam | 9.7 MB | ||
EGAF00005563981 | bam | 10.2 MB | ||
EGAF00005563982 | bam | 10.0 MB | ||
EGAF00005563983 | bam | 10.9 MB | ||
EGAF00005563984 | bam | 7.3 MB | ||
EGAF00005563985 | bam | 7.8 MB | ||
EGAF00005563986 | bam | 7.9 MB | ||
EGAF00005563987 | bam | 9.0 MB | ||
EGAF00005563988 | bam | 9.0 MB | ||
EGAF00005563989 | bam | 8.4 MB | ||
EGAF00005563990 | bam | 7.9 MB | ||
EGAF00005563991 | bam | 7.7 MB | ||
EGAF00005563992 | bam | 7.1 MB | ||
EGAF00005563993 | bam | 7.0 MB | ||
EGAF00005563994 | bam | 7.7 MB | ||
EGAF00005563995 | bam | 8.3 MB | ||
EGAF00005563996 | bam | 7.3 MB | ||
EGAF00005563997 | bam | 7.8 MB | ||
EGAF00005563998 | bam | 8.5 MB | ||
EGAF00005563999 | bam | 9.6 MB | ||
EGAF00005564000 | bam | 7.5 MB | ||
EGAF00005564001 | bam | 9.7 MB | ||
EGAF00005564002 | bam | 9.8 MB | ||
EGAF00005564003 | bam | 10.8 MB | ||
EGAF00005564004 | bam | 11.1 MB | ||
EGAF00005564005 | bam | 10.9 MB | ||
EGAF00005564006 | bam | 10.4 MB | ||
EGAF00005564007 | bam | 10.5 MB | ||
EGAF00005564008 | bam | 10.9 MB | ||
EGAF00005564009 | bam | 11.7 MB | ||
EGAF00005564010 | bam | 12.4 MB | ||
EGAF00005564011 | bam | 12.5 MB | ||
EGAF00005564012 | bam | 11.6 MB | ||
EGAF00005564013 | bam | 10.6 MB | ||
EGAF00005564014 | bam | 8.9 MB | ||
EGAF00005564015 | bam | 8.7 MB | ||
EGAF00005564016 | bam | 8.8 MB | ||
EGAF00005564017 | bam | 9.4 MB | ||
EGAF00005564018 | bam | 9.9 MB | ||
EGAF00005564019 | bam | 9.0 MB | ||
EGAF00005564020 | bam | 8.3 MB | ||
EGAF00005564021 | bam | 9.3 MB | ||
EGAF00005564022 | bam | 9.3 MB | ||
EGAF00005564023 | bam | 8.9 MB | ||
EGAF00005564024 | bam | 9.4 MB | ||
EGAF00005564025 | bam | 9.4 MB | ||
EGAF00005564026 | bam | 9.9 MB | ||
EGAF00005564027 | bam | 9.1 MB | ||
EGAF00005564028 | bam | 9.2 MB | ||
EGAF00005564029 | bam | 9.7 MB | ||
EGAF00005564030 | bam | 8.7 MB | ||
EGAF00005564031 | bam | 10.0 MB | ||
EGAF00005564032 | bam | 8.8 MB | ||
EGAF00005564033 | bam | 8.0 MB | ||
EGAF00005564034 | bam | 7.9 MB | ||
EGAF00005564035 | bam | 8.0 MB | ||
EGAF00005564036 | bam | 8.3 MB | ||
EGAF00005564037 | bam | 8.4 MB | ||
EGAF00005564038 | bam | 8.4 MB | ||
EGAF00005564039 | bam | 8.8 MB | ||
EGAF00005564040 | bam | 8.2 MB | ||
EGAF00005564041 | bam | 8.3 MB | ||
EGAF00005564042 | bam | 8.7 MB | ||
EGAF00005564043 | bam | 8.0 MB | ||
EGAF00005564044 | bam | 8.9 MB | ||
EGAF00005564045 | bam | 8.7 MB | ||
EGAF00005564046 | bam | 9.2 MB | ||
EGAF00005564047 | bam | 8.8 MB | ||
EGAF00005564048 | bam | 8.0 MB | ||
EGAF00005564049 | bam | 8.7 MB | ||
EGAF00005564050 | bam | 8.0 MB | ||
EGAF00005564051 | bam | 8.9 MB | ||
EGAF00005564052 | bam | 8.7 MB | ||
EGAF00005564053 | bam | 8.8 MB | ||
EGAF00005564054 | bam | 8.8 MB | ||
EGAF00005564055 | bam | 8.9 MB | ||
EGAF00005564056 | bam | 8.8 MB | ||
EGAF00005564057 | bam | 9.2 MB | ||
EGAF00005564058 | bam | 9.0 MB | ||
EGAF00005564059 | bam | 9.1 MB | ||
EGAF00005564060 | bam | 8.9 MB | ||
EGAF00005564061 | bam | 8.5 MB | ||
EGAF00005564062 | bam | 8.5 MB | ||
EGAF00005564063 | bam | 8.4 MB | ||
EGAF00005564064 | bam | 10.1 MB | ||
EGAF00005564065 | bam | 8.9 MB | ||
EGAF00005564066 | bam | 8.9 MB | ||
EGAF00005564067 | bam | 8.4 MB | ||
EGAF00005564068 | bam | 8.5 MB | ||
EGAF00005564069 | bam | 9.7 MB | ||
EGAF00005564070 | bam | 8.8 MB | ||
EGAF00005564071 | bam | 9.0 MB | ||
EGAF00005564072 | bam | 8.3 MB | ||
EGAF00005564073 | bam | 9.6 MB | ||
EGAF00005564074 | bam | 8.7 MB | ||
EGAF00005564075 | bam | 8.8 MB | ||
EGAF00005564076 | bam | 8.7 MB | ||
EGAF00005564077 | bam | 9.4 MB | ||
EGAF00005564078 | bam | 9.5 MB | ||
EGAF00005564079 | bam | 9.8 MB | ||
EGAF00005564080 | bam | 9.9 MB | ||
EGAF00005564081 | bam | 10.7 MB | ||
EGAF00005564082 | bam | 10.7 MB | ||
EGAF00005564083 | bam | 11.1 MB | ||
EGAF00005564084 | bam | 11.2 MB | ||
EGAF00005564085 | bam | 11.4 MB | ||
EGAF00005564086 | bam | 10.7 MB | ||
EGAF00005564087 | bam | 5.9 MB | ||
EGAF00005564088 | bam | 5.6 MB | ||
EGAF00005564089 | bam | 5.9 MB | ||
EGAF00005564090 | bam | 5.6 MB | ||
EGAF00005564091 | bam | 5.4 MB | ||
EGAF00005564092 | bam | 5.5 MB | ||
EGAF00005564093 | bam | 5.6 MB | ||
EGAF00005564094 | bam | 10.4 MB | ||
EGAF00005564095 | bam | 9.9 MB | ||
EGAF00005564096 | bam | 8.9 MB | ||
EGAF00005564097 | bam | 10.6 MB | ||
EGAF00005564098 | bam | 9.3 MB | ||
EGAF00005564099 | bam | 9.5 MB | ||
EGAF00005564100 | bam | 10.3 MB | ||
EGAF00005564101 | bam | 9.6 MB | ||
EGAF00005564102 | bam | 9.7 MB | ||
EGAF00005564103 | bam | 9.5 MB | ||
EGAF00005564104 | bam | 8.9 MB | ||
EGAF00005564105 | bam | 9.2 MB | ||
EGAF00005564106 | bam | 9.6 MB | ||
EGAF00005564107 | bam | 9.4 MB | ||
EGAF00005564108 | bam | 9.8 MB | ||
EGAF00005564109 | bam | 9.5 MB | ||
EGAF00005564110 | bam | 7.0 MB | ||
EGAF00005564111 | bam | 8.1 MB | ||
EGAF00005564112 | bam | 8.9 MB | ||
EGAF00005564113 | bam | 7.5 MB | ||
EGAF00005564114 | bam | 7.7 MB | ||
EGAF00005564115 | bam | 8.1 MB | ||
EGAF00005564116 | bam | 8.0 MB | ||
EGAF00005564117 | bam | 8.0 MB | ||
EGAF00005564118 | bam | 8.3 MB | ||
EGAF00005564119 | bam | 8.1 MB | ||
EGAF00005564120 | bam | 8.1 MB | ||
EGAF00005564121 | bam | 7.6 MB | ||
EGAF00005564122 | bam | 7.6 MB | ||
EGAF00005564123 | bam | 7.8 MB | ||
EGAF00005564124 | bam | 8.2 MB | ||
EGAF00005564125 | bam | 8.1 MB | ||
EGAF00005564126 | bam | 8.1 MB | ||
EGAF00005564127 | bam | 5.4 MB | ||
EGAF00005564128 | bam | 5.4 MB | ||
EGAF00005564129 | bam | 5.0 MB | ||
EGAF00005564130 | bam | 5.3 MB | ||
EGAF00005564131 | bam | 5.2 MB | ||
EGAF00005564132 | bam | 5.6 MB | ||
EGAF00005564133 | bam | 5.5 MB | ||
EGAF00005564134 | bam | 5.6 MB | ||
EGAF00005564135 | bam | 5.4 MB | ||
EGAF00005564136 | bam | 5.6 MB | ||
EGAF00005564137 | bam | 5.3 MB | ||
EGAF00005564138 | bam | 9.6 MB | ||
EGAF00005564139 | bam | 5.4 MB | ||
EGAF00005564140 | bam | 5.6 MB | ||
EGAF00005564141 | bam | 5.4 MB | ||
EGAF00005564142 | bam | 5.1 MB | ||
EGAF00005564143 | bam | 5.7 MB | ||
EGAF00005564144 | bam | 8.8 MB | ||
EGAF00005564145 | bam | 9.3 MB | ||
EGAF00005564146 | bam | 9.0 MB | ||
EGAF00005564147 | bam | 9.5 MB | ||
EGAF00005564148 | bam | 9.7 MB | ||
EGAF00005564149 | bam | 9.0 MB | ||
EGAF00005564150 | bam | 9.5 MB | ||
EGAF00005564151 | bam | 9.8 MB | ||
EGAF00005564152 | bam | 9.3 MB | ||
EGAF00005564153 | bam | 8.5 MB | ||
EGAF00005564154 | bam | 9.1 MB | ||
EGAF00005564155 | bam | 9.2 MB | ||
EGAF00005564156 | bam | 9.7 MB | ||
EGAF00005564157 | bam | 9.5 MB | ||
EGAF00005564158 | bam | 5.7 MB | ||
EGAF00005564159 | bam | 5.6 MB | ||
EGAF00005564160 | bam | 5.5 MB | ||
EGAF00005564161 | bam | 6.0 MB | ||
EGAF00005564162 | bam | 5.6 MB | ||
EGAF00005564163 | bam | 5.9 MB | ||
EGAF00005564164 | bam | 5.9 MB | ||
EGAF00005564165 | bam | 5.8 MB | ||
EGAF00005564166 | bam | 6.0 MB | ||
EGAF00005564167 | bam | 10.0 MB | ||
EGAF00005564168 | bam | 9.6 MB | ||
EGAF00005564169 | bam | 8.5 MB | ||
EGAF00005564170 | bam | 9.3 MB | ||
EGAF00005564171 | bam | 9.5 MB | ||
EGAF00005564172 | bam | 9.0 MB | ||
EGAF00005564173 | bam | 10.8 MB | ||
EGAF00005564174 | bam | 10.1 MB | ||
EGAF00005564175 | bam | 9.6 MB | ||
EGAF00005564176 | bam | 9.8 MB | ||
EGAF00005564177 | bam | 8.7 MB | ||
EGAF00005564178 | bam | 9.1 MB | ||
EGAF00005564179 | bam | 9.3 MB | ||
EGAF00005564180 | bam | 8.8 MB | ||
EGAF00005564181 | bam | 9.7 MB | ||
EGAF00005564182 | bam | 9.6 MB | ||
EGAF00005564183 | bam | 5.8 MB | ||
EGAF00005564184 | bam | 8.1 MB | ||
EGAF00005564185 | bam | 8.2 MB | ||
EGAF00005564186 | bam | 8.3 MB | ||
EGAF00005564187 | bam | 7.8 MB | ||
EGAF00005564188 | bam | 7.9 MB | ||
EGAF00005564189 | bam | 7.9 MB | ||
EGAF00005564190 | bam | 8.8 MB | ||
EGAF00005564191 | bam | 8.1 MB | ||
EGAF00005564192 | bam | 8.2 MB | ||
EGAF00005564193 | bam | 8.1 MB | ||
EGAF00005564194 | bam | 8.1 MB | ||
EGAF00005564195 | bam | 8.1 MB | ||
EGAF00005564196 | bam | 8.1 MB | ||
EGAF00005564197 | bam | 8.5 MB | ||
EGAF00005564198 | bam | 7.9 MB | ||
EGAF00005564199 | bam | 6.7 MB | ||
EGAF00005564200 | bam | 7.6 MB | ||
EGAF00005564201 | bam | 7.1 MB | ||
EGAF00005564202 | bam | 6.9 MB | ||
EGAF00005564203 | bam | 6.4 MB | ||
EGAF00005564204 | bam | 6.4 MB | ||
EGAF00005564205 | bam | 6.5 MB | ||
EGAF00005564206 | bam | 7.1 MB | ||
EGAF00005564207 | bam | 7.4 MB | ||
EGAF00005564208 | bam | 6.6 MB | ||
EGAF00005564209 | bam | 6.7 MB | ||
EGAF00005564210 | bam | 6.8 MB | ||
EGAF00005564211 | bam | 6.6 MB | ||
EGAF00005564212 | bam | 6.8 MB | ||
EGAF00005564213 | bam | 6.6 MB | ||
EGAF00005564214 | bam | 6.8 MB | ||
EGAF00005564215 | bam | 7.0 MB | ||
EGAF00005564216 | bam | 7.3 MB | ||
EGAF00005564217 | bam | 8.4 MB | ||
EGAF00005564218 | bam | 8.1 MB | ||
EGAF00005564219 | bam | 8.2 MB | ||
EGAF00005564220 | bam | 8.3 MB | ||
EGAF00005564221 | bam | 8.4 MB | ||
EGAF00005564222 | bam | 8.1 MB | ||
EGAF00005564223 | bam | 8.6 MB | ||
EGAF00005564224 | bam | 8.4 MB | ||
EGAF00005564225 | bam | 8.5 MB | ||
EGAF00005564226 | bam | 9.0 MB | ||
EGAF00005564227 | bam | 8.4 MB | ||
EGAF00005564228 | bam | 7.9 MB | ||
EGAF00005564229 | bam | 8.6 MB | ||
EGAF00005564230 | bam | 8.0 MB | ||
EGAF00005564231 | bam | 8.8 MB | ||
EGAF00005564232 | bam | 8.6 MB | ||
EGAF00005564233 | bam | 6.6 MB | ||
EGAF00005564234 | bam | 6.9 MB | ||
EGAF00005564235 | bam | 6.9 MB | ||
EGAF00005564236 | bam | 6.6 MB | ||
EGAF00005564237 | bam | 7.0 MB | ||
EGAF00005564238 | bam | 6.9 MB | ||
EGAF00005564239 | bam | 7.7 MB | ||
EGAF00005564240 | bam | 7.2 MB | ||
EGAF00005564241 | bam | 6.7 MB | ||
EGAF00005564242 | bam | 6.9 MB | ||
EGAF00005564243 | bam | 6.7 MB | ||
EGAF00005564244 | bam | 6.9 MB | ||
EGAF00005564245 | bam | 6.6 MB | ||
EGAF00005564246 | bam | 6.8 MB | ||
EGAF00005564247 | bam | 6.6 MB | ||
EGAF00005564248 | bam | 6.9 MB | ||
EGAF00005564249 | bam | 7.0 MB | ||
EGAF00005564250 | bam | 5.9 MB | ||
EGAF00005564251 | bam | 6.3 MB | ||
EGAF00005564252 | bam | 6.6 MB | ||
EGAF00005564253 | bam | 6.4 MB | ||
EGAF00005564254 | bam | 6.0 MB | ||
EGAF00005564255 | bam | 6.0 MB | ||
EGAF00005564256 | bam | 6.1 MB | ||
EGAF00005564257 | bam | 6.3 MB | ||
EGAF00005564258 | bam | 6.2 MB | ||
EGAF00005564259 | bam | 6.3 MB | ||
EGAF00005564260 | bam | 6.6 MB | ||
EGAF00005564261 | bam | 6.2 MB | ||
EGAF00005564262 | bam | 6.5 MB | ||
EGAF00005564263 | bam | 6.1 MB | ||
EGAF00005564264 | bam | 6.2 MB | ||
EGAF00005564265 | bam | 6.3 MB | ||
EGAF00005564266 | bam | 6.5 MB | ||
EGAF00005564267 | bam | 6.1 MB | ||
EGAF00005564268 | bam | 5.9 MB | ||
EGAF00005564269 | bam | 6.1 MB | ||
EGAF00005564270 | bam | 6.2 MB | ||
EGAF00005564271 | bam | 7.3 MB | ||
EGAF00005564272 | bam | 5.7 MB | ||
EGAF00005564273 | bam | 6.1 MB | ||
EGAF00005564274 | bam | 6.6 MB | ||
EGAF00005564275 | bam | 6.4 MB | ||
EGAF00005564276 | bam | 6.1 MB | ||
EGAF00005564277 | bam | 6.2 MB | ||
EGAF00005564278 | bam | 6.2 MB | ||
EGAF00005564279 | bam | 6.9 MB | ||
EGAF00005564280 | bam | 6.3 MB | ||
EGAF00005564281 | bam | 6.5 MB | ||
EGAF00005564282 | bam | 6.6 MB | ||
EGAF00005564283 | bam | 5.5 MB | ||
EGAF00005564284 | bam | 6.6 MB | ||
EGAF00005564285 | bam | 7.0 MB | ||
EGAF00005564286 | bam | 6.8 MB | ||
EGAF00005564287 | bam | 6.7 MB | ||
EGAF00005564288 | bam | 6.9 MB | ||
EGAF00005564289 | bam | 7.3 MB | ||
EGAF00005564290 | bam | 6.6 MB | ||
EGAF00005564291 | bam | 7.3 MB | ||
EGAF00005564292 | bam | 7.8 MB | ||
EGAF00005564293 | bam | 7.0 MB | ||
EGAF00005564294 | bam | 7.0 MB | ||
EGAF00005564295 | bam | 6.9 MB | ||
EGAF00005564296 | bam | 7.0 MB | ||
EGAF00005564297 | bam | 6.9 MB | ||
EGAF00005564298 | bam | 7.1 MB | ||
EGAF00005564299 | bam | 6.9 MB | ||
EGAF00005564300 | bam | 6.9 MB | ||
EGAF00005564301 | bam | 7.3 MB | ||
EGAF00005564302 | bam | 7.8 MB | ||
EGAF00005564303 | bam | 7.2 MB | ||
EGAF00005564304 | bam | 7.3 MB | ||
EGAF00005564305 | bam | 7.4 MB | ||
EGAF00005564306 | bam | 7.1 MB | ||
EGAF00005564307 | bam | 7.0 MB | ||
EGAF00005564308 | bam | 7.0 MB | ||
EGAF00005564309 | bam | 7.2 MB | ||
EGAF00005564310 | bam | 7.7 MB | ||
EGAF00005564311 | bam | 7.0 MB | ||
EGAF00005564312 | bam | 7.4 MB | ||
EGAF00005564313 | bam | 7.0 MB | ||
EGAF00005564314 | bam | 6.9 MB | ||
EGAF00005564315 | bam | 7.3 MB | ||
EGAF00005564316 | bam | 7.0 MB | ||
EGAF00005564317 | bam | 6.5 MB | ||
EGAF00005564318 | bam | 7.6 MB | ||
EGAF00005564319 | bam | 7.1 MB | ||
EGAF00005564320 | bam | 6.6 MB | ||
EGAF00005564321 | bam | 7.1 MB | ||
EGAF00005564322 | bam | 7.5 MB | ||
EGAF00005564323 | bam | 7.3 MB | ||
EGAF00005564324 | bam | 8.0 MB | ||
EGAF00005564325 | bam | 7.2 MB | ||
EGAF00005564326 | bam | 6.1 MB | ||
EGAF00005564327 | bam | 6.8 MB | ||
EGAF00005564328 | bam | 8.9 MB | ||
EGAF00005564329 | bam | 9.4 MB | ||
EGAF00005564330 | bam | 7.4 MB | ||
EGAF00005564331 | bam | 7.9 MB | ||
EGAF00005564332 | bam | 7.7 MB | ||
EGAF00005564333 | bam | 7.6 MB | ||
EGAF00005564334 | bam | 5.1 MB | ||
EGAF00005564335 | bam | 7.4 MB | ||
EGAF00005564336 | bam | 8.9 MB | ||
EGAF00005564337 | bam | 7.9 MB | ||
EGAF00005564338 | bam | 8.4 MB | ||
EGAF00005564339 | bam | 7.8 MB | ||
EGAF00005564340 | bam | 6.8 MB | ||
EGAF00005564341 | bam | 8.9 MB | ||
EGAF00005564342 | bam | 7.4 MB | ||
EGAF00005564343 | bam | 3.2 MB | ||
EGAF00005564344 | bam | 5.8 MB | ||
EGAF00005564345 | bam | 7.2 MB | ||
EGAF00005564346 | bam | 6.7 MB | ||
EGAF00005564347 | bam | 6.4 MB | ||
EGAF00005564348 | bam | 6.4 MB | ||
EGAF00005564349 | bam | 5.6 MB | ||
EGAF00005564350 | bam | 6.6 MB | ||
EGAF00005564351 | bam | 7.0 MB | ||
EGAF00005564352 | bam | 6.3 MB | ||
EGAF00005564353 | bam | 7.9 MB | ||
EGAF00005564354 | bam | 5.9 MB | ||
EGAF00005564355 | bam | 8.0 MB | ||
EGAF00005564356 | bam | 7.8 MB | ||
EGAF00005564357 | bam | 6.4 MB | ||
EGAF00005564358 | bam | 4.9 MB | ||
EGAF00005564359 | bam | 7.6 MB | ||
EGAF00005564360 | bam | 8.3 MB | ||
EGAF00005564361 | bam | 9.4 MB | ||
EGAF00005564362 | bam | 4.5 MB | ||
EGAF00005564363 | bam | 6.3 MB | ||
EGAF00005564364 | bam | 6.4 MB | ||
EGAF00005564365 | bam | 5.4 MB | ||
EGAF00005564366 | bam | 4.2 MB | ||
EGAF00005564367 | bam | 4.4 MB | ||
EGAF00005564368 | bam | 6.2 MB | ||
EGAF00005564369 | bam | 6.6 MB | ||
EGAF00005564370 | bam | 4.8 MB | ||
EGAF00005564371 | bam | 7.2 MB | ||
EGAF00005564372 | bam | 6.4 MB | ||
EGAF00005564373 | bam | 7.5 MB | ||
EGAF00005564374 | bam | 6.5 MB | ||
EGAF00005564375 | bam | 6.7 MB | ||
EGAF00005564376 | bam | 7.2 MB | ||
EGAF00005564377 | bam | 6.7 MB | ||
EGAF00005564378 | bam | 6.6 MB | ||
EGAF00005564379 | bam | 6.8 MB | ||
EGAF00005564380 | bam | 7.9 MB | ||
EGAF00005564381 | bam | 7.9 MB | ||
EGAF00005564382 | bam | 5.9 MB | ||
EGAF00005564383 | bam | 7.7 MB | ||
EGAF00005564384 | bam | 7.9 MB | ||
EGAF00005564385 | bam | 7.2 MB | ||
EGAF00005564386 | bam | 8.7 MB | ||
EGAF00005564387 | bam | 8.3 MB | ||
EGAF00005564388 | bam | 6.2 MB | ||
EGAF00005564389 | bam | 6.8 MB | ||
EGAF00005564390 | bam | 6.6 MB | ||
EGAF00005564391 | bam | 6.7 MB | ||
EGAF00005564392 | bam | 7.2 MB | ||
EGAF00005564393 | bam | 7.2 MB | ||
EGAF00005564394 | bam | 6.4 MB | ||
EGAF00005564395 | bam | 6.5 MB | ||
EGAF00005564396 | bam | 7.6 MB | ||
EGAF00005564397 | bam | 7.0 MB | ||
EGAF00005564398 | bam | 6.1 MB | ||
EGAF00005564399 | bam | 6.6 MB | ||
EGAF00005564400 | bam | 5.6 MB | ||
EGAF00005564401 | bam | 5.7 MB | ||
EGAF00005564402 | bam | 5.9 MB | ||
EGAF00005564403 | bam | 6.9 MB | ||
EGAF00005564404 | bam | 6.4 MB | ||
EGAF00005564405 | bam | 7.4 MB | ||
EGAF00005564406 | bam | 950.6 kB | ||
EGAF00005564407 | bam | 6.5 MB | ||
EGAF00005564408 | bam | 5.6 MB | ||
EGAF00005564409 | bam | 4.8 MB | ||
EGAF00005564410 | bam | 7.3 MB | ||
EGAF00005564411 | bam | 5.7 MB | ||
EGAF00005564412 | bam | 5.2 MB | ||
EGAF00005564413 | bam | 3.3 MB | ||
EGAF00005564414 | bam | 4.2 MB | ||
EGAF00005564415 | bam | 5.6 MB | ||
EGAF00005564416 | bam | 3.7 MB | ||
EGAF00005564417 | bam | 6.0 MB | ||
EGAF00005564418 | bam | 6.7 MB | ||
EGAF00005564419 | bam | 9.1 MB | ||
EGAF00005564420 | bam | 6.6 MB | ||
EGAF00005564421 | bam | 7.4 MB | ||
EGAF00005564422 | bam | 6.9 MB | ||
EGAF00005564423 | bam | 6.7 MB | ||
EGAF00005564424 | bam | 6.3 MB | ||
EGAF00005564425 | bam | 5.5 MB | ||
EGAF00005564426 | bam | 8.6 MB | ||
EGAF00005564427 | bam | 8.3 MB | ||
EGAF00005564428 | bam | 8.2 MB | ||
EGAF00005564429 | bam | 7.3 MB | ||
EGAF00005564430 | bam | 8.4 MB | ||
EGAF00005564431 | bam | 8.7 MB | ||
EGAF00005564432 | bam | 8.3 MB | ||
EGAF00005564433 | bam | 3.9 MB | ||
EGAF00005564434 | bam | 9.4 MB | ||
EGAF00005564435 | bam | 8.0 MB | ||
EGAF00005564436 | bam | 8.0 MB | ||
EGAF00005564437 | bam | 8.4 MB | ||
EGAF00005564438 | bam | 8.3 MB | ||
EGAF00005564439 | bam | 8.1 MB | ||
EGAF00005564440 | bam | 7.9 MB | ||
EGAF00005564441 | bam | 7.6 MB | ||
EGAF00005564442 | bam | 8.3 MB | ||
EGAF00005564443 | bam | 7.6 MB | ||
EGAF00005564444 | bam | 6.7 MB | ||
EGAF00005564445 | bam | 6.4 MB | ||
EGAF00005564446 | bam | 7.2 MB | ||
EGAF00005564447 | bam | 9.5 MB | ||
EGAF00005564448 | bam | 8.7 MB | ||
EGAF00005564449 | bam | 7.4 MB | ||
EGAF00005564450 | bam | 5.9 MB | ||
EGAF00005564451 | bam | 7.1 MB | ||
EGAF00005564452 | bam | 5.8 MB | ||
EGAF00005564453 | bam | 6.2 MB | ||
EGAF00005564454 | bam | 6.6 MB | ||
EGAF00005564455 | bam | 6.6 MB | ||
EGAF00005564456 | bam | 5.5 MB | ||
EGAF00005564457 | bam | 6.6 MB | ||
EGAF00005564458 | bam | 6.0 MB | ||
EGAF00005564459 | bam | 5.4 MB | ||
EGAF00005564460 | bam | 6.6 MB | ||
EGAF00005564461 | bam | 6.8 MB | ||
EGAF00005564462 | bam | 5.9 MB | ||
EGAF00005564463 | bam | 6.5 MB | ||
EGAF00005564464 | bam | 5.2 MB | ||
EGAF00005564465 | bam | 5.9 MB | ||
EGAF00005564466 | bam | 5.9 MB | ||
EGAF00005564467 | bam | 6.1 MB | ||
EGAF00005564468 | bam | 6.0 MB | ||
EGAF00005564469 | bam | 6.0 MB | ||
EGAF00005564470 | bam | 5.9 MB | ||
EGAF00005564471 | bam | 5.9 MB | ||
EGAF00005564472 | bam | 6.9 MB | ||
EGAF00005564473 | bam | 7.2 MB | ||
707 Files (5.5 GB) |