Single cell transcriptomic data of human gut macrophages

Cellular suspensions (∼15000 cells, with expected recovery of ∼7500 cells) of sorted CD45+ HLA-DR+ CD14+ macrophages from colonic mucosa and muscularis propria were loaded on the 10X Chromium Controller instrument (10X Genomics) according to the manufacturer’s protocol using the 10X GEMCode proprietary technology. All samples from individual patients were loaded in one batch. The Chromium Single Cell 3´ v2 Reagent kit (10X Genomics) was used to generate the cDNA and prepare the libraries, according to the manufacturer’s protocol. The libraries were then equimolarly pooled and sequenced on an Illumina NextSeq500 using HighOutput flow cells v2.5. A coverage of 400M reads per sample was targeted, in order to obtain 50 000 reads per cell. The raw data were then demultiplexed and processed with the Cell Ranger software (10X Genomics) v2.1.1.

8 samples
DAC: EGAC00001002166
Technology: NextSeq 500

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Study ID	Study Title	Study Type
EGAS00001005377	Spatiotemporal single cell transcriptomic analysis of human gut macrophages reveals multiple functional and niche-specific subsets	RNASeq

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID	File Type	Size
EGAF00005316713	bam	18.2 GB
EGAF00005316714	bam	15.6 GB
EGAF00005316715	bam	32.5 GB
EGAF00005316716	bam	18.0 GB
EGAF00005316717	bam	15.8 GB
EGAF00005316718	bam	17.0 GB
EGAF00005316719	bam	27.0 GB
EGAF00005316720	bam	11.3 GB
8 Files (155.5 GB)