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1M-scBloodNL

Here we present the 1M-scBloodNL study, in which we performed single-cell RNA-seq on 120 individuals of the Northern Netherlands population cohort Lifelines. For each individual peripheral blood mononuclear cells (PBMCs) were sequenced in an unstimulated condition, and after 3 and 24 hour in vitro stimulation with C. albicans (CA), M. tuberculosis (MTB) and P. aeruginosa (PA), totalling approximately 1.3 million cells. scRNA-seq was conducted with the 10X Genomics 3'-end v2 (72 libraries) and v3 (33 libraries) technology. In general, each library contains PBMCs from 8 donors and 2 different stimulation-timepoint combinations. Donors were demultiplexed using a combination of SoupOrCell (https://www.nature.com/articles/s41592-020-0820-1) and genotype information to assign the correct donor to a donor-specific cell cluster.

Request Access

Genotype and gene expression data are freely available. Single-cell RNA-seq data (fastq files), including a tsv file that links each cell barcode to its respective donor and stimulation-timepoint combination, has been deposited at the European Genome-phenome Archive (EGA). Genotype data is hosted from the HPC cluster of the UMCG.

The 1M-scBloodNL Data Access Committee (DAC) determines access permission. In order to request data access, please click on request data and fill in the data request form with a signed data access agreement that can be found on https://eqtlgen.org/sc/datasets/1m-scbloodnl.html We aim to provide data access within 5 working days. However, during the holiday season there might be a slight delay. For more information, contact Monique van der Wijst (m.g.p.van.der.wijst@umcg.nl).

Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00005316416 1623403315237 6.1 GB
EGAF00005316417 1623448552730 22.7 GB
EGAF00005316418 1623422165673 8.8 GB
EGAF00005316419 1623445266267 34.1 GB
EGAF00005316420 1623410332448 11.0 GB
EGAF00005316421 1623458851680 41.3 GB
EGAF00005316422 1623400849479 2.0 GB
EGAF00005316423 1623401083493 7.4 GB
EGAF00005316424 1623412251992 7.3 GB
EGAF00005316425 1623451766823 28.0 GB
EGAF00005316426 1623403065619 5.3 GB
EGAF00005316427 1623429717593 23.9 GB
EGAF00005316428 1623401083493 6.2 GB
EGAF00005316429 1623434962757 28.8 GB
EGAF00005316430 1623412731941 5.6 GB
EGAF00005316431 1623430250532 25.1 GB
EGAF00005316432 1623406367589 5.6 GB
EGAF00005316433 1623421807351 21.0 GB
EGAF00005316434 1623409737463 5.8 GB
EGAF00005316435 1623439290183 26.7 GB
EGAF00005316436 1623407929270 8.2 GB
EGAF00005316437 1623448012343 31.4 GB
EGAF00005316438 1623403429418 2.7 GB
EGAF00005316439 1623412379084 10.0 GB
EGAF00005316440 1623407929270 8.7 GB
EGAF00005316441 1623447609637 32.2 GB
EGAF00005316442 1623413995337 4.8 GB
EGAF00005316443 1623432300395 21.9 GB
EGAF00005316444 1623403551744 2.1 GB
EGAF00005316445 1623418026736 7.8 GB
EGAF00005316446 1623404750416 5.1 GB
EGAF00005316447 1623427004780 21.8 GB
EGAF00005316448 1623420784560 9.0 GB
EGAF00005316449 1623434456897 34.8 GB
EGAF00005316450 1623406034173 5.8 GB
EGAF00005316451 1623406604333 25.5 GB
EGAF00005316452 1623401805286 1.8 GB
EGAF00005316453 1623418148262 6.7 GB
EGAF00005316454 1623413363778 8.1 GB
EGAF00005316455 1623407446086 31.1 GB
EGAF00005316456 1623401205076 5.7 GB
EGAF00005316457 1623444845642 25.9 GB
EGAF00005316458 1623423421555 8.0 GB
EGAF00005316459 1623458222577 29.7 GB
EGAF00005316460 1623404272398 5.8 GB
EGAF00005316461 1623412014512 21.6 GB
EGAF00005316462 1623409977941 5.9 GB
EGAF00005316463 1623442676908 26.8 GB
EGAF00005316464 1623414598796 5.7 GB
EGAF00005316465 1623440053551 26.1 GB
EGAF00005316466 1623406137061 3.8 GB
EGAF00005316467 1623419277789 14.0 GB
EGAF00005316468 1623409132740 10.4 GB
EGAF00005316469 1623455700894 39.4 GB
EGAF00005316470 1623400730303 2.5 GB
EGAF00005316471 1623402552649 9.2 GB
EGAF00005316472 1623406137061 11.3 GB
EGAF00005316473 1623404157805 42.4 GB
EGAF00005316474 1623407465178 8.7 GB
EGAF00005316475 1623442497257 33.3 GB
EGAF00005316476 1623431398426 10.3 GB
EGAF00005316477 1623445266267 39.3 GB
EGAF00005316478 1623404396278 2.3 GB
EGAF00005316479 1623410090453 8.7 GB
EGAF00005316480 1623403801535 2.5 GB
EGAF00005316481 1623406725560 9.6 GB
EGAF00005316482 1623403188832 6.3 GB
EGAF00005316483 1623439868934 29.1 GB
EGAF00005316484 1623406034173 5.5 GB
EGAF00005316485 1623434092967 25.1 GB
EGAF00005316486 1623409737463 5.9 GB
EGAF00005316487 1623433377417 21.9 GB
EGAF00005316488 1623419527421 8.1 GB
EGAF00005316489 1623447789872 31.3 GB
EGAF00005316490 1623406367589 9.2 GB
EGAF00005316491 1623445637983 34.5 GB
EGAF00005316492 1623408405086 10.4 GB
EGAF00005316493 1623449521562 39.0 GB
EGAF00005316494 1623404030520 5.9 GB
EGAF00005316495 1623433912598 21.8 GB
EGAF00005316496 1623411895627 6.1 GB
EGAF00005316497 1623446288179 27.8 GB
EGAF00005316498 1623420431748 5.5 GB
EGAF00005316499 1623428454392 24.6 GB
EGAF00005316500 1623409737463 6.1 GB
EGAF00005316501 1623414956660 28.6 GB
EGAF00005316502 1623400864617 5.4 GB
EGAF00005316503 1623413363778 25.2 GB
EGAF00005316504 1623412379084 5.5 GB
EGAF00005316505 1623443648616 24.9 GB
EGAF00005316506 1623407325774 5.6 GB
EGAF00005316507 1623436256613 26.2 GB
EGAF00005316508 1623401330003 5.0 GB
EGAF00005316509 1623421384501 23.0 GB
EGAF00005316510 1623414837760 9.7 GB
EGAF00005316511 1623467894735 36.5 GB
EGAF00005316512 1623403551744 2.4 GB
EGAF00005316513 1623403204310 9.0 GB
EGAF00005316514 1623404396278 6.2 GB
EGAF00005316515 1623434456897 28.4 GB
EGAF00005316516 1623404396278 11.2 GB
EGAF00005316517 1623444482805 42.7 GB
EGAF00005316518 1623405008078 3.7 GB
EGAF00005316519 1623419277789 13.4 GB
EGAF00005316520 1623406034173 2.9 GB
EGAF00005316521 1623423122797 10.7 GB
EGAF00005316522 1623414234793 5.4 GB
EGAF00005316523 1623422525011 19.7 GB
EGAF00005316524 1623403915263 5.1 GB
EGAF00005316525 1623449521562 24.0 GB
EGAF00005316526 1623409737463 8.3 GB
EGAF00005316527 1623453851211 31.2 GB
EGAF00005316528 1623412379084 10.9 GB
EGAF00005316529 1623417445641 40.0 GB
EGAF00005316530 1623406485208 5.9 GB
EGAF00005316531 1623425196785 27.7 GB
EGAF00005316532 1623409737463 5.6 GB
EGAF00005316533 1623414234793 26.6 GB
EGAF00005316534 1623405170601 5.9 GB
EGAF00005316535 1623448931216 26.7 GB
EGAF00005316536 1623420431748 5.7 GB
EGAF00005316537 1623432659113 26.3 GB
EGAF00005316538 1623405277139 5.3 GB
EGAF00005316539 1623424133887 24.2 GB
EGAF00005316540 1623405064394 5.4 GB
EGAF00005316541 1623443648616 24.3 GB
EGAF00005316542 1623402439555 5.1 GB
EGAF00005316543 1623440826449 23.4 GB
EGAF00005316544 1623412014512 11.0 GB
EGAF00005316545 1623445266267 41.0 GB
EGAF00005316546 1623413363778 6.6 GB
EGAF00005316547 1623438097125 30.2 GB
EGAF00005316548 1623406970502 5.9 GB
EGAF00005316549 1623448748436 27.3 GB
EGAF00005316550 1623425937891 9.6 GB
EGAF00005316551 1623447609637 36.7 GB
EGAF00005316552 1623415921583 11.0 GB
EGAF00005316553 1623440279605 42.2 GB
EGAF00005316554 1623405170601 5.4 GB
EGAF00005316555 1623444066711 23.6 GB
EGAF00005316556 1623402724755 2.7 GB
EGAF00005316557 1623406367589 9.7 GB
EGAF00005316558 1623404272398 6.1 GB
EGAF00005316559 1623448012343 27.7 GB
EGAF00005316560 1623404272398 6.0 GB
EGAF00005316561 1623422468843 26.3 GB
EGAF00005316562 1623409009341 4.9 GB
EGAF00005316563 1623447414532 22.7 GB
EGAF00005316564 1623406034173 5.8 GB
EGAF00005316565 1623422468843 22.1 GB
EGAF00005316566 1623418866011 10.1 GB
EGAF00005316567 1623454503144 38.4 GB
EGAF00005316568 1623406034173 5.9 GB
EGAF00005316569 1623441013359 27.3 GB
EGAF00005316570 1623407446086 6.1 GB
EGAF00005316571 1623469323686 27.5 GB
EGAF00005316572 1623402439557 5.7 GB
EGAF00005316573 1623434962757 26.3 GB
EGAF00005316574 1623406034173 1.9 GB
EGAF00005316575 1623419527421 6.9 GB
EGAF00005316576 1623401805286 4.8 GB
EGAF00005316577 1623439290183 22.2 GB
EGAF00005316578 1623401450015 2.2 GB
EGAF00005316579 1623416951561 8.0 GB
EGAF00005316580 1623413632520 8.5 GB
EGAF00005316581 1623415804236 32.9 GB
EGAF00005316582 1623404157805 6.9 GB
EGAF00005316583 1623408532162 31.9 GB
EGAF00005316584 1623404396278 5.6 GB
EGAF00005316585 1623405008078 25.0 GB
EGAF00005316586 1623405008078 5.3 GB
EGAF00005316587 1623413363778 23.7 GB
EGAF00005316588 1623406604333 7.6 GB
EGAF00005316589 1623431935299 27.7 GB
EGAF00005316590 1623402709256 8.2 GB
EGAF00005316591 1623406034173 31.0 GB
EGAF00005316592 1623402552649 6.1 GB
EGAF00005316593 1623448198756 28.2 GB
EGAF00005316594 1623416156153 5.5 GB
EGAF00005316595 1623442497257 20.1 GB
EGAF00005316596 1623404750416 6.0 GB
EGAF00005316597 1623449711873 27.3 GB
EGAF00005316598 1623403801535 6.0 GB
EGAF00005316599 1623439290183 22.3 GB
EGAF00005316600 1623407929270 8.3 GB
EGAF00005316601 1623454503144 31.6 GB
EGAF00005316602 1623412731941 10.5 GB
EGAF00005316603 1623420784560 39.6 GB
EGAF00005316604 1623401690592 8.1 GB
EGAF00005316605 1623453049974 29.5 GB
EGAF00005316606 1623414715611 5.3 GB
EGAF00005316607 1623404750416 24.0 GB
EGAF00005316608 1623401083493 5.6 GB
EGAF00005316609 1623411895627 25.4 GB
EGAF00005316610 1623402709256 6.4 GB
EGAF00005316611 1623403315237 30.4 GB
EGAF00005316612 1623403315237 5.8 GB
EGAF00005316613 1623437158288 21.7 GB
EGAF00005316614 1623407465178 8.0 GB
EGAF00005316615 1623453049974 31.0 GB
EGAF00005316616 1623409737463 6.1 GB
EGAF00005316617 1623428094527 22.5 GB
EGAF00005316618 1623406034173 7.6 GB
EGAF00005316619 1623438097125 28.2 GB
EGAF00005316620 1623405517621 5.8 GB
EGAF00005316621 1623435005531 21.6 GB
EGAF00005316622 1623402439555 10.7 GB
EGAF00005316623 1623438097125 40.3 GB
EGAF00005316624 1623406034173 8.4 GB
EGAF00005316625 1623449521562 32.5 GB
EGAF00005316626 1623408643829 7.5 GB
EGAF00005316627 1623430429825 28.7 GB
EGAF00005316628 1623404883749 9.4 GB
EGAF00005316629 1623412379084 35.6 GB
EGAF00005316630 1623426645813 10.6 GB
EGAF00005316631 1623418319933 40.8 GB
EGAF00005316632 1623401205076 9.4 GB
EGAF00005316633 1623424133887 35.7 GB
EGAF00005316634 1623405517621 5.7 GB
EGAF00005316635 1623425047521 26.0 GB
EGAF00005316636 1623402439557 7.7 GB
EGAF00005316637 1623451992858 29.5 GB
EGAF00005316638 1623413879443 4.8 GB
EGAF00005316639 1623404030520 22.0 GB
EGAF00005316640 1623410811963 6.9 GB
EGAF00005316641 1623439499707 32.6 GB
EGAF00005316642 1623403065619 11.6 GB
EGAF00005316643 1623444066711 43.6 GB
EGAF00005316644 1623402951513 6.0 GB
EGAF00005316645 1623442497257 27.4 GB
EGAF00005316646 1623405517621 5.5 GB
EGAF00005316647 1623406725560 25.2 GB
EGAF00005316648 1623406970502 10.3 GB
EGAF00005316649 1623447414532 39.1 GB
EGAF00005316650 1623407325774 5.7 GB
EGAF00005316651 1623405008078 26.5 GB
EGAF00005316652 1623404750416 5.9 GB
EGAF00005316653 1623402439558 27.7 GB
EGAF00005316654 1623412251992 10.7 GB
EGAF00005316655 1623455468536 40.8 GB
EGAF00005316656 1623413995337 5.4 GB
EGAF00005316657 1623439290183 19.8 GB
EGAF00005316658 1623401933553 2.8 GB
EGAF00005316659 1623418026736 10.3 GB
EGAF00005316660 1623403188832 5.5 GB
EGAF00005316661 1623439868934 25.3 GB
EGAF00005316662 1623408643829 5.5 GB
EGAF00005316663 1623444303868 25.0 GB
EGAF00005316664 1623414474077 6.1 GB
EGAF00005316665 1623427195439 27.7 GB
EGAF00005316666 1623400974662 6.2 GB
EGAF00005316667 1623439290183 28.3 GB
EGAF00005316668 1623403429418 5.9 GB
EGAF00005316669 1623413480828 27.1 GB
EGAF00005316670 1623415432765 6.0 GB
EGAF00005316671 1623431935299 27.5 GB
EGAF00005316672 1623406034173 5.8 GB
EGAF00005316673 1623426283790 26.0 GB
EGAF00005316674 1623404633593 5.4 GB
EGAF00005316675 1623417109109 24.7 GB
EGAF00005316676 1623410697908 8.3 GB
EGAF00005316677 1623440053551 31.9 GB
EGAF00005316678 1623404272398 5.4 GB
EGAF00005316679 1623409737463 24.5 GB
EGAF00005316680 1623401450015 5.8 GB
EGAF00005316681 1623427719026 27.7 GB
EGAF00005316682 1623411290441 5.5 GB
EGAF00005316683 1623408288381 25.4 GB
EGAF00005316684 1623405008078 5.5 GB
EGAF00005316685 1623404396278 20.2 GB
EGAF00005316686 1623406137061 2.7 GB
EGAF00005316687 1623413363778 10.2 GB
EGAF00005316688 1623405008078 6.0 GB
EGAF00005316689 1623435717077 27.1 GB
EGAF00005316690 1623403429418 5.8 GB
EGAF00005316691 1623436619588 26.4 GB
EGAF00005316692 1623400374314 5.4 GB
EGAF00005316693 1623422649141 25.2 GB
EGAF00005316694 1623409024241 5.5 GB
EGAF00005316695 1623426645813 25.7 GB
EGAF00005316696 1623413363778 8.2 GB
EGAF00005316697 1623441245635 31.7 GB
EGAF00005316698 1623402552649 5.0 GB
EGAF00005316699 1623441013359 18.3 GB
EGAF00005316700 1623402439558 5.5 GB
EGAF00005316701 1623446691821 25.4 GB
EGAF00005316702 1623414598796 5.5 GB
EGAF00005316703 1623420726197 26.0 GB
EGAF00005316704 1623415432765 6.2 GB
EGAF00005316705 1623412379084 28.2 GB
EGAF00005316706 1623415795459 5.7 GB
EGAF00005316707 1623425196785 25.5 GB
EGAF00005316708 1623411064210 8.6 GB
EGAF00005316709 1623448931216 33.1 GB
EGAF00005316710 1623409737463 9.7 GB
EGAF00005316711 1623423421555 35.6 GB
EGAF00005316712 1623399299395 8.6 kB
297 Files (4.9 TB)