Single Cell RNA sequencing of Organoids from TSC2+/- patient IPSCs in H- and L-medium

Data Access Policy for IMBA is as follows. The following questions will be evaluated by the Data Access Committee for IMBA: Does the research project comply with the original informed consent? Project's ethical approval Security and confidentiality of the local environment Preventing re-identity of the subject from the published data Accept or Deny Contract between requester and corresponding author Authorization tool

Data Access EGA studies consist of one or more datasets. Each dataset is affiliated to a Data Access Committee (DAC), which is the body responsible for all data access decisions. Data Access Comitte for IMBA Contact persons: ethics@imba.oeaw.ac.at Contact persons: Arabella Meixner/Nina Malajner Data Access Policy: Does the research project comply with the original informed consent? Project's ethical approval Security and confidentiality of the local environment Preventing re-identity of the subject from the published data Accept or Deny Contract between requester and corresponding author Authorization tool Ethical principles IMBA fulfills all legal requirements as Research Institute in Austria that is allowed by Austrian law to use, process, collect and import human cells and human tissue. This study is designed and shall be implemented and reported in accordance with the ICH (International Conference on Harmonization) Harmonized Tripartite Guidelines for Good Clinical Practice, with the ethical principles stated in the Declaration of Helsinki. All procedures will be performed after patients’ written informed consent and after approval by the local national ethic committee/Institutional Review Board following the national, international and EU ethics regulations. The samples received have been pseudonymized/anonymized at the local institutions and thus cannot be retracked by any IMBA employee. The identity and other types of personal information of the patients will be treated strictly confidential. All required licenses necessary for the execution of the project are in place. Their validity is monitored by our “Ethics and Biosafety” Department. If applicable, their renewal process will be initiated in time to guarantee continuous validity. The group leader and all scientists will respect the highest standards of research integrity and will ensure: to present all research goals in an honest and transparent manner to design the research carefully and conduct it in a reliable fashion to use appropriate techniques and methodologies (including for data management) to exercise due care for the subjects of research to ensure objectivity, accuracy and impartiality while disseminating to make the necessary references to refrain from plagiarism, data falsification or fabrication to avoid double funding, conflicts of interest and misrepresentation of credentials All persons involved in research are obliged to adhere to the principles of integrity in research and scholarship as well as to the Standards of Good Scientific Practice. IMBA, via the membership of the Austrian Academy of Sciences, has adopted guidelines and procedural regulations of the Austrian Agency for Research Integrity https://oeawi.at/richtlinien/”.