Whole exome sequencing data from non-small-cell lung cancer patients receiving immunotherapy prognosis
The plasma samples and white blood cell samples were collected from 30 non-small-cell lung cancer patients and 3 healthy individuals. The solid tumor biopsy samples from 14 patients (a subset of the 30 patients) were collected. The cfDNA was extracted from their plasma samples using the QIAamp circulating nucleic acid kit from QIAGEN (Germantown, MD). The cfDNA WES library was constructed with the SureSelect XT HS kit from Agilent Technologies (Santa Clara, CA) according to the manufacturer’s protocol. In brief, 10ng of cfDNA was used as input material. After end repair/dA-tailing of cfDNA, the adaptor was ligated. The ligation product was purified with Ampure XP beads (Beckman-Coulter, Atlanta, GA) and the adaptor-ligated library was amplified with index primer in 10-cycle PCR. The amplified library was purified again with Ampure XP beads, and the amount of amplified DNA was measured using the Qubit 1xdsDNA HS assay kit (ThermoFisher, Waltham, MA). 700-1000 ng of DNA sample was hybridized to the Agilent SureSelect Human All Exon V6 (Agilent) capture library and pulled down by streptavidin-coated beads. After washing the beads, the DNA library captured on the beads was re-amplified with 10-cycle PCR. The final libraries were purified by Ampure XP beads. The library concentration was measured by Qubit, and the quality was further examined with Agilent Bioanalyzer before the final step of 2x150bp paired-end sequencing on the Illumina HiSeq X10 platform (Illumina) at an average coverage of 200. Whole-exome capture libraries of genomic DNA of the 30 non-small cell lung cancer patients were constructed via Roche SeqCap EZ Exome V3 (Roche); whole-exome capture libraries of genomic DNA of the 3 healthy individuals were constructed via Agilent SureSelect Human All Exon V6 (Agilent). Enriched exome libraries were sequenced on the Illumina HiSeq 3000 platform (Illumina) to generate 2x100bp paired-end reads at an average coverage of 200.
- 83 samples
- DAC: EGAC00001001569
- Technologies: HiSeq X Ten, Illumina HiSeq 3000
The data usage policy for whole-exome sequencing data of cell-free DNA derived from non-small-cell lung cancer patients receiving immunotherapies
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| EGAS00001004373 | Other |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF00003620566 | fastq.gz | 6.0 GB | ||
| EGAF00003620567 | fastq.gz | 6.7 GB | ||
| EGAF00003620568 | fastq.gz | 7.2 GB | ||
| EGAF00003620569 | fastq.gz | 7.9 GB | ||
| EGAF00003620570 | fastq.gz | 7.3 GB | ||
| EGAF00003620571 | fastq.gz | 8.0 GB | ||
| EGAF00003620572 | fastq.gz | 8.3 GB | ||
| EGAF00003620573 | fastq.gz | 9.2 GB | ||
| EGAF00003620574 | fastq.gz | 8.2 GB | ||
| EGAF00003620575 | fastq.gz | 9.1 GB | ||
| EGAF00003620576 | fastq.gz | 7.5 GB | ||
| EGAF00003620577 | fastq.gz | 8.3 GB | ||
| EGAF00003620578 | fastq.gz | 7.0 GB | ||
| EGAF00003620579 | fastq.gz | 7.7 GB | ||
| EGAF00003620580 | fastq.gz | 7.5 GB | ||
| EGAF00003620581 | fastq.gz | 8.3 GB | ||
| EGAF00003620582 | fastq.gz | 4.9 GB | ||
| EGAF00003620583 | fastq.gz | 5.4 GB | ||
| EGAF00003620584 | fastq.gz | 9.9 GB | ||
| EGAF00003620585 | fastq.gz | 10.7 GB | ||
| EGAF00003620586 | fastq.gz | 6.1 GB | ||
| EGAF00003620587 | fastq.gz | 6.6 GB | ||
| EGAF00003620588 | fastq.gz | 8.4 GB | ||
| EGAF00003620589 | fastq.gz | 9.0 GB | ||
| EGAF00003620590 | fastq.gz | 8.3 GB | ||
| EGAF00003620591 | fastq.gz | 8.9 GB | ||
| EGAF00003620592 | fastq.gz | 4.6 GB | ||
| EGAF00003620593 | fastq.gz | 4.9 GB | ||
| EGAF00003620594 | fastq.gz | 2.7 GB | ||
| EGAF00003620595 | fastq.gz | 2.9 GB | ||
| EGAF00003620596 | fastq.gz | 10.2 GB | ||
| EGAF00003620597 | fastq.gz | 11.0 GB | ||
| EGAF00003620598 | fastq.gz | 6.2 GB | ||
| EGAF00003620599 | fastq.gz | 6.6 GB | ||
| EGAF00003620600 | fastq.gz | 9.0 GB | ||
| EGAF00003620601 | fastq.gz | 9.5 GB | ||
| EGAF00003620602 | fastq.gz | 7.9 GB | ||
| EGAF00003620603 | fastq.gz | 8.3 GB | ||
| EGAF00003620604 | fastq.gz | 10.6 GB | ||
| EGAF00003620605 | fastq.gz | 11.3 GB | ||
| EGAF00003620606 | fastq.gz | 7.2 GB | ||
| EGAF00003620607 | fastq.gz | 7.7 GB | ||
| EGAF00003620608 | fastq.gz | 8.1 GB | ||
| EGAF00003620609 | fastq.gz | 8.6 GB | ||
| EGAF00003620610 | fastq.gz | 7.9 GB | ||
| EGAF00003620611 | fastq.gz | 8.4 GB | ||
| EGAF00003620612 | fastq.gz | 7.6 GB | ||
| EGAF00003620613 | fastq.gz | 8.1 GB | ||
| EGAF00003620614 | fastq.gz | 8.5 GB | ||
| EGAF00003620615 | fastq.gz | 9.0 GB | ||
| EGAF00003620616 | fastq.gz | 9.9 GB | ||
| EGAF00003620617 | fastq.gz | 10.6 GB | ||
| EGAF00003620618 | fastq.gz | 8.6 GB | ||
| EGAF00003620619 | fastq.gz | 9.2 GB | ||
| EGAF00003620620 | fastq.gz | 8.4 GB | ||
| EGAF00003620621 | fastq.gz | 9.0 GB | ||
| EGAF00003620622 | fastq.gz | 9.6 GB | ||
| EGAF00003620623 | fastq.gz | 10.2 GB | ||
| EGAF00003620624 | fastq.gz | 6.6 GB | ||
| EGAF00003620625 | fastq.gz | 7.0 GB | ||
| EGAF00003620626 | fastq.gz | 7.0 GB | ||
| EGAF00003620627 | fastq.gz | 7.8 GB | ||
| EGAF00003620628 | fastq.gz | 5.8 GB | ||
| EGAF00003620629 | fastq.gz | 6.2 GB | ||
| EGAF00003620630 | fastq.gz | 6.7 GB | ||
| EGAF00003620631 | fastq.gz | 6.8 GB | ||
| EGAF00003620632 | fastq.gz | 8.8 GB | ||
| EGAF00003620633 | fastq.gz | 10.0 GB | ||
| EGAF00003620634 | fastq.gz | 11.9 GB | ||
| EGAF00003620635 | fastq.gz | 13.3 GB | ||
| EGAF00003620636 | fastq.gz | 12.0 GB | ||
| EGAF00003620637 | fastq.gz | 13.5 GB | ||
| EGAF00003620638 | fastq.gz | 12.5 GB | ||
| EGAF00003620639 | fastq.gz | 14.3 GB | ||
| EGAF00003620640 | fastq.gz | 7.4 GB | ||
| EGAF00003620641 | fastq.gz | 7.9 GB | ||
| EGAF00003620642 | fastq.gz | 10.8 GB | ||
| EGAF00003620643 | fastq.gz | 12.2 GB | ||
| EGAF00003620644 | fastq.gz | 13.5 GB | ||
| EGAF00003620645 | fastq.gz | 15.6 GB | ||
| EGAF00003620646 | fastq.gz | 8.4 GB | ||
| EGAF00003620647 | fastq.gz | 8.3 GB | ||
| EGAF00003620648 | fastq.gz | 9.4 GB | ||
| EGAF00003620649 | fastq.gz | 9.4 GB | ||
| EGAF00003620650 | fastq.gz | 7.3 GB | ||
| EGAF00003620651 | fastq.gz | 8.5 GB | ||
| EGAF00003620652 | fastq.gz | 8.6 GB | ||
| EGAF00003620653 | fastq.gz | 8.6 GB | ||
| EGAF00003620654 | fastq.gz | 6.3 GB | ||
| EGAF00003620655 | fastq.gz | 7.1 GB | ||
| EGAF00003620656 | fastq.gz | 12.5 GB | ||
| EGAF00003620657 | fastq.gz | 13.9 GB | ||
| EGAF00003620658 | fastq.gz | 14.4 GB | ||
| EGAF00003620659 | fastq.gz | 16.5 GB | ||
| EGAF00003620660 | fastq.gz | 12.2 GB | ||
| EGAF00003620661 | fastq.gz | 13.6 GB | ||
| EGAF00003620662 | fastq.gz | 7.0 GB | ||
| EGAF00003620663 | fastq.gz | 7.3 GB | ||
| EGAF00003620664 | fastq.gz | 5.6 GB | ||
| EGAF00003620665 | fastq.gz | 5.6 GB | ||
| EGAF00003620666 | fastq.gz | 5.2 GB | ||
| EGAF00003620667 | fastq.gz | 5.2 GB | ||
| EGAF00003620668 | fastq.gz | 8.8 GB | ||
| EGAF00003620669 | fastq.gz | 8.8 GB | ||
| EGAF00003620670 | fastq.gz | 7.3 GB | ||
| EGAF00003620671 | fastq.gz | 7.3 GB | ||
| EGAF00003620672 | fastq.gz | 8.2 GB | ||
| EGAF00003620673 | fastq.gz | 8.2 GB | ||
| EGAF00003620674 | fastq.gz | 4.9 GB | ||
| EGAF00003620675 | fastq.gz | 5.1 GB | ||
| EGAF00003620676 | fastq.gz | 8.4 GB | ||
| EGAF00003620677 | fastq.gz | 9.1 GB | ||
| EGAF00003620678 | fastq.gz | 6.3 GB | ||
| EGAF00003620679 | fastq.gz | 6.3 GB | ||
| EGAF00003620680 | fastq.gz | 6.0 GB | ||
| EGAF00003620681 | fastq.gz | 6.0 GB | ||
| EGAF00003620682 | fastq.gz | 6.6 GB | ||
| EGAF00003620683 | fastq.gz | 6.6 GB | ||
| EGAF00003620684 | fastq.gz | 8.7 GB | ||
| EGAF00003620685 | fastq.gz | 9.4 GB | ||
| EGAF00003620686 | fastq.gz | 11.3 GB | ||
| EGAF00003620687 | fastq.gz | 12.4 GB | ||
| EGAF00003620688 | fastq.gz | 10.0 GB | ||
| EGAF00003620689 | fastq.gz | 9.9 GB | ||
| EGAF00003620690 | fastq.gz | 14.4 GB | ||
| EGAF00003620691 | fastq.gz | 14.4 GB | ||
| EGAF00003620692 | fastq.gz | 11.0 GB | ||
| EGAF00003620693 | fastq.gz | 11.0 GB | ||
| EGAF00003620694 | fastq.gz | 7.0 GB | ||
| EGAF00003620695 | fastq.gz | 6.9 GB | ||
| EGAF00003620696 | fastq.gz | 9.3 GB | ||
| EGAF00003620697 | fastq.gz | 10.3 GB | ||
| EGAF00003620698 | fastq.gz | 4.8 GB | ||
| EGAF00003620699 | fastq.gz | 4.7 GB | ||
| EGAF00003620700 | fastq.gz | 11.9 GB | ||
| EGAF00003620701 | fastq.gz | 11.9 GB | ||
| EGAF00003620702 | fastq.gz | 12.8 GB | ||
| EGAF00003620703 | fastq.gz | 12.8 GB | ||
| EGAF00003620704 | fastq.gz | 4.7 GB | ||
| EGAF00003620705 | fastq.gz | 4.7 GB | ||
| EGAF00003620706 | fastq.gz | 6.2 GB | ||
| EGAF00003620707 | fastq.gz | 6.2 GB | ||
| EGAF00003620708 | fastq.gz | 5.3 GB | ||
| EGAF00003620709 | fastq.gz | 5.3 GB | ||
| EGAF00003620710 | fastq.gz | 8.5 GB | ||
| EGAF00003620711 | fastq.gz | 8.5 GB | ||
| EGAF00003620712 | fastq.gz | 8.5 GB | ||
| EGAF00003620713 | fastq.gz | 9.4 GB | ||
| EGAF00005186875 | fastq.gz | 7.2 GB | ||
| EGAF00005186876 | fastq.gz | 8.2 GB | ||
| EGAF00005186877 | fastq.gz | 6.7 GB | ||
| EGAF00005186878 | fastq.gz | 7.6 GB | ||
| EGAF00005186879 | fastq.gz | 6.7 GB | ||
| EGAF00005186880 | fastq.gz | 7.7 GB | ||
| EGAF00005186881 | fastq.gz | 7.9 GB | ||
| EGAF00005186882 | fastq.gz | 8.9 GB | ||
| EGAF00005186883 | fastq.gz | 7.0 GB | ||
| EGAF00005186884 | fastq.gz | 8.1 GB | ||
| EGAF00005186885 | fastq.gz | 6.6 GB | ||
| EGAF00005186886 | fastq.gz | 7.6 GB | ||
| EGAF00005186887 | fastq.gz | 6.8 GB | ||
| EGAF00005186888 | fastq.gz | 7.6 GB | ||
| EGAF00005186889 | fastq.gz | 6.5 GB | ||
| EGAF00005186890 | fastq.gz | 7.4 GB | ||
| EGAF00005186891 | fastq.gz | 6.9 GB | ||
| EGAF00005186892 | fastq.gz | 7.8 GB | ||
| 166 Files (1.4 TB) | ||||