RNA-seq of non-LPS treated (N), non-tolerized (NT), and tolerized (T) IFNg-primed macrophages pretreated with or without HDAC3i
In this experiment we investigated the effect of HDAC3 inhibition on the transcriptome of IFNg-primed macrophages under different tolerization conditions. Peripheral blood mononuclear cells (PBMCs) were isolated from 3 healthy donors. PBMCs were isolated from whole blood of healthy donors using Ficoll gradient (Invitrogen). Monocytes (CD14+ cells) were positively selected from PBMCs using CD14 Microbeads according to the manufacturer’s instructions (Miltenyi Biotec). Monocytes were subsequently treated with or without 500 nM HDAC3i (ITF3100) for 30 minutes prior to overnight IFNg priming (50 ng/mL). Cells were then kept without LPS (non-LPS; N), treated with 10 ng/mL LPS once (non-tolerized; NT), or treated with LPS twice (tolerized; T; second LPS concentration: 100 ng/mL). In total, there were 18 samples included.
- 18 samples
- DAC: EGAC00001001483
- Technology: Illumina HiSeq 4000
Controlled access to data generated at the Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, University of Amsterdam.
- The User Institution agrees to protect the confidentiality of Research Participants in any research papers or publications that they prepare by taking all reasonable care to limit the possibility of identification. - The User Institution agrees not to link or combine these Data to other information or archived data available in a way that could re-identify the Research Participants, even if access to that data has been formally granted to the User Institution or is freely available without restriction. Any user requesting access to the data generated at the Tytgat Institute for Liver and Intestinal Research must apply for authorization, which is granted by the Tytgat Institute for Liver and Intestinal Research Data Access Committee (DAC). Decisions to grant access are made based on whether the request conforms to the specifications within the Data Access Agreement
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| RNASeq |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF00003446593 | fastq.gz | 1.5 GB | ||
| EGAF00003446594 | fastq.gz | 1.6 GB | ||
| EGAF00003446595 | fastq.gz | 1.7 GB | ||
| EGAF00003446596 | fastq.gz | 1.5 GB | ||
| EGAF00003446597 | fastq.gz | 1.6 GB | ||
| EGAF00003446598 | fastq.gz | 1.7 GB | ||
| EGAF00003446599 | fastq.gz | 1.6 GB | ||
| EGAF00003446600 | fastq.gz | 1.5 GB | ||
| EGAF00003446601 | fastq.gz | 1.7 GB | ||
| EGAF00003446602 | fastq.gz | 1.5 GB | ||
| EGAF00003446603 | fastq.gz | 1.3 GB | ||
| EGAF00003446604 | fastq.gz | 1.6 GB | ||
| EGAF00003446605 | fastq.gz | 1.6 GB | ||
| EGAF00003446606 | fastq.gz | 1.4 GB | ||
| EGAF00003446607 | fastq.gz | 1.4 GB | ||
| EGAF00003446608 | fastq.gz | 1.6 GB | ||
| EGAF00003446609 | fastq.gz | 1.7 GB | ||
| EGAF00003446610 | fastq.gz | 1.6 GB | ||
| EGAF00003446651 | bam | 838.0 MB | ||
| EGAF00003446652 | bai | 2.4 MB | ||
| EGAF00003446653 | bam | 985.3 MB | ||
| EGAF00003446654 | bai | 2.4 MB | ||
| EGAF00003446655 | bam | 958.6 MB | ||
| EGAF00003446656 | bai | 2.4 MB | ||
| EGAF00003446705 | bam | 846.1 MB | ||
| EGAF00003446706 | bai | 2.4 MB | ||
| EGAF00003446707 | bam | 1.0 GB | ||
| EGAF00003446708 | bai | 2.4 MB | ||
| EGAF00003446709 | bam | 986.1 MB | ||
| EGAF00003446710 | bai | 2.4 MB | ||
| EGAF00003446711 | bam | 1.1 GB | ||
| EGAF00003446712 | bai | 2.4 MB | ||
| EGAF00003446713 | bam | 966.4 MB | ||
| EGAF00003446714 | bai | 2.5 MB | ||
| EGAF00003446755 | bam | 991.9 MB | ||
| EGAF00003446756 | bai | 4.1 MB | ||
| EGAF00003446757 | bam | 866.5 MB | ||
| EGAF00003446758 | bai | 2.4 MB | ||
| EGAF00003446759 | bam | 999.1 MB | ||
| EGAF00003446760 | bai | 2.5 MB | ||
| EGAF00003446761 | bam | 1.0 GB | ||
| EGAF00003446762 | bai | 2.5 MB | ||
| EGAF00003446763 | bam | 1.0 GB | ||
| EGAF00003446764 | bai | 2.5 MB | ||
| EGAF00003446765 | bam | 1.0 GB | ||
| EGAF00003446766 | bai | 2.5 MB | ||
| EGAF00003446876 | bam | 922.3 MB | ||
| EGAF00003446877 | bai | 2.4 MB | ||
| EGAF00003446878 | bam | 961.0 MB | ||
| EGAF00003446879 | bai | 2.5 MB | ||
| EGAF00003446880 | bam | 997.1 MB | ||
| EGAF00003446881 | bai | 2.5 MB | ||
| EGAF00003446882 | bam | 941.8 MB | ||
| EGAF00003446883 | bai | 2.4 MB | ||
| 54 Files (45.4 GB) | ||||