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Analysis of the B cell receptor repertoire in six immune-mediated diseases

Our understanding of the BCR repertoire in the context of immune-mediated diseases is incomplete, and defining this could provide new insights into pathogenesis and therapy. Here, we compared the BCR repertoire in systemic lupus erythematosus, anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis, Crohn’s disease, Behçet’s disease, eosinophilic granulomatosis with polyangiitis, and immunoglobulin A (IgA) vasculitis by analysing BCR clonality, use of immunoglobulin heavy-chain variable region (IGHV) genes and—in particular—isotype use. An increase in clonality in systemic lupus erythematosus and Crohn’s disease that was dominated by the IgA isotype, together with skewed use of the IGHV genes in these and other diseases, suggested a microbial contribution to pathogenesis. Different immunosuppressive treatments had specific and distinct effects on the repertoire; B cells that persisted after treatment with rituximab were predominately isotype-switched and clonally expanded, whereas the inverse was true for B cells that persisted after treatment with mycophenolate mofetil. Our comparative analysis of the BCR repertoire in immune- mediated disease reveals a complex B cell architecture, providing a platform for understanding pathological mechanisms and designing treatment strategies.

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
EGAS00001003185 Resequencing

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00001752691 cram 276.3 MB
EGAF00001752692 cram 488.9 MB
EGAF00001752729 cram 120.3 MB
EGAF00001752730 cram 100.8 MB
EGAF00001752731 cram 137.3 MB
EGAF00001752732 cram 101.5 MB
EGAF00001752734 cram 105.5 MB
EGAF00001752738 cram 120.8 MB
EGAF00001752743 cram 372.1 MB
EGAF00001752744 cram 402.2 MB
EGAF00001752745 cram 373.0 MB
EGAF00001752746 cram 375.9 MB
EGAF00001752747 cram 504.0 MB
EGAF00001752748 cram 376.2 MB
EGAF00001752803 cram 273.9 MB
EGAF00001752805 cram 245.0 MB
EGAF00001752806 cram 216.3 MB
EGAF00001752810 cram 258.2 MB
EGAF00001752811 cram 248.2 MB
EGAF00001752812 cram 243.3 MB
EGAF00001752814 cram 230.1 MB
EGAF00001752815 cram 246.7 MB
EGAF00001752816 cram 263.7 MB
EGAF00001752817 cram 208.7 MB
EGAF00001752818 cram 257.3 MB
EGAF00001752822 cram 241.5 MB
EGAF00001752825 cram 237.8 MB
EGAF00001752826 cram 250.7 MB
EGAF00001752829 cram 227.1 MB
EGAF00001752830 cram 308.9 MB
EGAF00001752832 cram 313.2 MB
EGAF00001752837 cram 286.7 MB
EGAF00001752838 cram 82.0 MB
EGAF00001752839 cram 93.7 MB
EGAF00001752841 cram 65.5 MB
EGAF00001752845 cram 88.3 MB
EGAF00001752849 cram 87.9 MB
EGAF00001752853 cram 113.2 MB
EGAF00001752855 cram 75.8 MB
EGAF00001752856 cram 85.9 MB
EGAF00001752857 cram 87.6 MB
EGAF00001752858 cram 99.7 MB
EGAF00001752859 cram 74.4 MB
EGAF00001752861 cram 98.5 MB
EGAF00001752862 cram 96.2 MB
EGAF00001752865 cram 88.0 MB
EGAF00001752867 cram 74.1 MB
EGAF00001752871 cram 87.8 MB
EGAF00001752873 cram 338.0 MB
EGAF00001752874 cram 357.2 MB
EGAF00001752875 cram 195.7 MB
EGAF00001752877 cram 167.7 MB
EGAF00001752878 cram 157.3 MB
EGAF00001752879 cram 215.8 MB
EGAF00001752880 cram 160.9 MB
EGAF00001752882 cram 190.9 MB
EGAF00001752883 cram 219.0 MB
EGAF00001752885 cram 182.3 MB
EGAF00001752886 cram 172.0 MB
EGAF00001752887 cram 155.9 MB
EGAF00001752888 cram 192.3 MB
EGAF00001752890 cram 170.8 MB
EGAF00001752891 cram 193.8 MB
EGAF00001752892 cram 195.5 MB
EGAF00001752893 cram 167.4 MB
EGAF00001752894 cram 213.6 MB
EGAF00001752895 cram 185.6 MB
EGAF00001752896 cram 113.3 MB
EGAF00001752898 cram 167.3 MB
EGAF00001752901 cram 172.0 MB
EGAF00001752903 cram 129.7 MB
EGAF00001752904 cram 115.0 MB
EGAF00001752906 cram 175.9 MB
EGAF00001752907 cram 145.3 MB
EGAF00001752908 cram 248.6 MB
EGAF00001752910 cram 191.7 MB
EGAF00001752911 cram 109.6 MB
EGAF00001752912 cram 205.6 MB
EGAF00001752913 cram 178.8 MB
EGAF00001752914 cram 125.1 MB
EGAF00001752915 cram 215.1 MB
EGAF00001752916 cram 187.4 MB
EGAF00001752917 cram 233.1 MB
EGAF00001752918 cram 260.4 MB
EGAF00001752919 cram 213.7 MB
EGAF00001752920 cram 287.5 MB
EGAF00001752921 cram 106.1 MB
EGAF00001752922 cram 53.6 MB
EGAF00001752925 cram 846.2 MB
EGAF00001752926 cram 966.5 MB
EGAF00001752928 cram 931.4 MB
EGAF00001752929 cram 969.1 MB
EGAF00001752930 cram 624.8 MB
EGAF00001752931 cram 288.3 MB
EGAF00001752932 cram 434.2 MB
EGAF00001752933 cram 417.9 MB
EGAF00001752935 cram 471.3 MB
EGAF00001752936 cram 463.0 MB
EGAF00001752938 cram 414.8 MB
EGAF00001752939 cram 431.7 MB
EGAF00001752940 cram 399.9 MB
EGAF00001752941 cram 346.7 MB
EGAF00001752942 cram 539.2 MB
EGAF00001752944 cram 447.7 MB
EGAF00001752945 cram 490.1 MB
EGAF00001752946 cram 588.4 MB
EGAF00001752947 cram 577.5 MB
EGAF00001752948 cram 563.4 MB
EGAF00001752950 cram 379.4 MB
EGAF00001752951 cram 383.6 MB
EGAF00001752952 cram 575.1 MB
EGAF00001752953 cram 555.1 MB
EGAF00001752954 cram 587.6 MB
EGAF00001752955 cram 678.2 MB
EGAF00001752956 cram 160.0 MB
EGAF00001752958 cram 138.7 MB
EGAF00001752959 cram 130.0 MB
EGAF00001752960 cram 177.5 MB
EGAF00001752961 cram 132.7 MB
EGAF00001752963 cram 153.8 MB
EGAF00001752964 cram 176.3 MB
EGAF00001752966 cram 155.4 MB
EGAF00001752967 cram 144.3 MB
EGAF00001752968 cram 131.5 MB
EGAF00001752969 cram 156.0 MB
EGAF00001752971 cram 141.1 MB
EGAF00001752972 cram 158.3 MB
EGAF00001752973 cram 157.5 MB
EGAF00001752974 cram 129.9 MB
EGAF00001752975 cram 166.7 MB
EGAF00001752976 cram 153.3 MB
EGAF00001752977 cram 96.6 MB
EGAF00001752979 cram 135.9 MB
EGAF00001752982 cram 141.3 MB
EGAF00001752984 cram 106.3 MB
EGAF00001752985 cram 100.6 MB
EGAF00001752987 cram 142.1 MB
EGAF00001752988 cram 122.5 MB
EGAF00001752989 cram 197.5 MB
EGAF00001752991 cram 150.1 MB
EGAF00001752992 cram 88.6 MB
EGAF00001752993 cram 161.6 MB
EGAF00001752994 cram 152.7 MB
EGAF00001752995 cram 142.9 MB
EGAF00001752996 cram 118.2 MB
EGAF00001752997 cram 176.6 MB
EGAF00001752998 cram 130.5 MB
EGAF00001753000 cram 113.7 MB
EGAF00001753004 cram 146.6 MB
EGAF00001753009 cram 470.3 MB
EGAF00001753010 cram 491.7 MB
EGAF00001753038 cram 486.2 MB
EGAF00001753039 cram 477.4 MB
EGAF00001753040 cram 630.5 MB
EGAF00001753041 cram 459.6 MB
EGAF00001753042 cram 427.9 MB
EGAF00001753043 cram 466.3 MB
EGAF00001753044 cram 100.8 MB
EGAF00001753045 cram 157.5 MB
EGAF00001753046 cram 145.2 MB
EGAF00001753047 cram 165.1 MB
EGAF00001753048 cram 186.4 MB
EGAF00001753049 cram 150.2 MB
EGAF00001753050 cram 197.5 MB
EGAF00001753051 cram 78.5 MB
EGAF00001753052 cram 40.4 MB
EGAF00001753055 cram 604.5 MB
EGAF00001753056 cram 685.4 MB
EGAF00001753058 cram 622.3 MB
EGAF00001753059 cram 658.8 MB
EGAF00001753060 cram 604.5 MB
EGAF00001753061 cram 266.9 MB
EGAF00001753062 cram 411.7 MB
EGAF00001753063 cram 403.1 MB
EGAF00001753065 cram 454.9 MB
EGAF00001753066 cram 444.1 MB
EGAF00001753097 cram 388.7 MB
EGAF00001753098 cram 403.8 MB
EGAF00001753099 cram 373.1 MB
EGAF00001753100 cram 335.3 MB
EGAF00001753101 cram 519.0 MB
EGAF00001753103 cram 456.1 MB
EGAF00001753104 cram 479.1 MB
EGAF00001753105 cram 571.8 MB
EGAF00001753106 cram 550.2 MB
EGAF00001753107 cram 549.3 MB
EGAF00001753109 cram 383.9 MB
EGAF00001753110 cram 372.7 MB
EGAF00001753111 cram 576.6 MB
EGAF00001753112 cram 544.8 MB
EGAF00001753113 cram 565.0 MB
EGAF00001753114 cram 651.9 MB
EGAF00001753115 cram 323.4 MB
EGAF00001753159 cram 278.8 MB
EGAF00001753161 cram 314.4 MB
EGAF00001753165 cram 212.3 MB
EGAF00001753167 cram 254.8 MB
EGAF00001753168 cram 226.5 MB
EGAF00001753174 cram 269.2 MB
EGAF00001753177 cram 266.4 MB
EGAF00001753178 cram 271.6 MB
EGAF00001753179 cram 267.5 MB
EGAF00001753181 cram 305.7 MB
EGAF00001753182 cram 293.4 MB
EGAF00002828028 cram 77.3 MB
EGAF00002828029 cram 95.0 MB
EGAF00002828030 cram 92.1 MB
EGAF00002828031 cram 70.6 MB
EGAF00002828032 cram 91.7 MB
EGAF00002828033 cram 73.9 MB
EGAF00002828034 cram 86.5 MB
EGAF00002828035 cram 77.1 MB
EGAF00002828036 cram 83.3 MB
EGAF00002828037 cram 70.1 MB
EGAF00002828038 cram 84.3 MB
EGAF00002828039 cram 82.8 MB
EGAF00002828040 cram 89.6 MB
EGAF00002828041 cram 73.7 MB
EGAF00002828042 cram 63.6 MB
EGAF00002828043 cram 135.6 MB
EGAF00002828044 cram 104.2 MB
EGAF00002828045 cram 124.6 MB
EGAF00002828046 cram 136.1 MB
EGAF00002828047 cram 132.7 MB
EGAF00002828048 cram 128.9 MB
EGAF00002828049 cram 98.2 MB
EGAF00002828050 cram 123.9 MB
EGAF00002828051 cram 132.1 MB
EGAF00002828052 cram 92.6 MB
EGAF00002828053 cram 92.9 MB
EGAF00002828054 cram 172.6 MB
EGAF00002828055 cram 145.3 MB
EGAF00002828056 cram 103.3 MB
EGAF00002828057 cram 230.0 MB
EGAF00002828058 cram 295.9 MB
EGAF00002828059 cram 217.3 MB
EGAF00002828060 cram 214.4 MB
EGAF00002828061 cram 164.7 MB
EGAF00002828062 cram 213.1 MB
EGAF00002828063 cram 225.0 MB
EGAF00002828064 cram 232.1 MB
EGAF00002828065 cram 253.9 MB
EGAF00002828066 cram 215.8 MB
EGAF00002828067 cram 232.0 MB
EGAF00002828068 cram 215.9 MB
245 Files (63.6 GB)