Whole transcriptome RNA sequencing on bone marrow and peripheral blood samples from patients with acute myeloid leukemia at diagnosis or relapse.
One hundred cryopreserved bone marrow and peripheral blood samples from patients with acute myeloid leukemia (AML) with 10-90% blasts were selected from the biobank of the Department of Hematology of Leiden University Medical Center (LUMC). The AML cases cover all subtypes, and specifically include known subtype-defining balanced chromosomal translocations according to the WHO classification. The samples were obtained from 96 patients and include three pairs of de novo and relapsed AML and one pair of de novo and presumed therapy-related AML (tAML). Total RNA was isolated from mononuclear cells without prior enrichment for leukemic blasts. The quality and integrity of total RNA was checked and RNA libraries were prepared using the TruSeq RNA library preparation kit v2 (Illumina, San Diego, CA) in an ISO/IEC 17025-accredited protocol. This workflow started with enrichment of messenger RNA by oligo dT magnetic beads. After fragmentation, cDNA synthesis was performed, followed by adaptor ligation and PCR amplification. Paired-end sequencing with a read length of 126 bp was performed on an Illumina HiSeq 2500 v4 sequencer to at least 12.5 Gbp per sample. Image analysis, base calling, and quality check was performed with Illumina data analysis pipeline RTA v1.18.64 and Bcl2fastq v1.8.4. RNAseq reads are provided in compressed Sanger FASTQ format.
- 21/04/2020
- 100 samples
- DAC: EGAC00001000956
- Technology: Illumina HiSeq 2500
Policy: Whole transcriptome RNA sequencing as comprehensive diagnostic tool for acute myeloid leukemia.
Restricted access because the study concerns patient data which should not be traced back to the patients. We will require citation plus acknowledgement.
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| Other |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF00002044247 | fastq.gz | 4.6 GB | ||
| EGAF00002044248 | fastq.gz | 4.7 GB | ||
| EGAF00002044249 | fastq.gz | 4.5 GB | ||
| EGAF00002044250 | fastq.gz | 4.6 GB | ||
| EGAF00002044251 | fastq.gz | 4.5 GB | ||
| EGAF00002044252 | fastq.gz | 4.6 GB | ||
| EGAF00002044253 | fastq.gz | 4.9 GB | ||
| EGAF00002044254 | fastq.gz | 5.1 GB | ||
| EGAF00002044255 | fastq.gz | 5.1 GB | ||
| EGAF00002044256 | fastq.gz | 5.3 GB | ||
| EGAF00002044257 | fastq.gz | 5.7 GB | ||
| EGAF00002044258 | fastq.gz | 6.1 GB | ||
| EGAF00002044259 | fastq.gz | 4.7 GB | ||
| EGAF00002044260 | fastq.gz | 4.7 GB | ||
| EGAF00002044261 | fastq.gz | 5.6 GB | ||
| EGAF00002044262 | fastq.gz | 5.6 GB | ||
| EGAF00002044263 | fastq.gz | 5.0 GB | ||
| EGAF00002044264 | fastq.gz | 4.8 GB | ||
| EGAF00002044265 | fastq.gz | 4.8 GB | ||
| EGAF00002044266 | fastq.gz | 4.9 GB | ||
| EGAF00002044267 | fastq.gz | 5.1 GB | ||
| EGAF00002044268 | fastq.gz | 5.0 GB | ||
| EGAF00002044269 | fastq.gz | 4.6 GB | ||
| EGAF00002044270 | fastq.gz | 4.5 GB | ||
| EGAF00002044271 | fastq.gz | 5.3 GB | ||
| EGAF00002044272 | fastq.gz | 5.3 GB | ||
| EGAF00002044273 | fastq.gz | 5.5 GB | ||
| EGAF00002044274 | fastq.gz | 5.4 GB | ||
| EGAF00002044275 | fastq.gz | 4.5 GB | ||
| EGAF00002044276 | fastq.gz | 4.5 GB | ||
| EGAF00002044277 | fastq.gz | 5.0 GB | ||
| EGAF00002044278 | fastq.gz | 5.0 GB | ||
| EGAF00002044279 | fastq.gz | 4.3 GB | ||
| EGAF00002044280 | fastq.gz | 4.3 GB | ||
| EGAF00002044281 | fastq.gz | 5.6 GB | ||
| EGAF00002044282 | fastq.gz | 5.6 GB | ||
| EGAF00002044283 | fastq.gz | 6.1 GB | ||
| EGAF00002044284 | fastq.gz | 6.2 GB | ||
| EGAF00002044285 | fastq.gz | 5.8 GB | ||
| EGAF00002044286 | fastq.gz | 5.6 GB | ||
| EGAF00002044287 | fastq.gz | 5.3 GB | ||
| EGAF00002044288 | fastq.gz | 5.3 GB | ||
| EGAF00002044289 | fastq.gz | 4.4 GB | ||
| EGAF00002044290 | fastq.gz | 4.4 GB | ||
| EGAF00002044291 | fastq.gz | 5.4 GB | ||
| EGAF00002044292 | fastq.gz | 5.3 GB | ||
| EGAF00002044293 | fastq.gz | 4.5 GB | ||
| EGAF00002044294 | fastq.gz | 4.4 GB | ||
| EGAF00002044295 | fastq.gz | 5.2 GB | ||
| EGAF00002044296 | fastq.gz | 5.1 GB | ||
| EGAF00002044297 | fastq.gz | 7.1 GB | ||
| EGAF00002044298 | fastq.gz | 7.1 GB | ||
| EGAF00002044299 | fastq.gz | 5.7 GB | ||
| EGAF00002044300 | fastq.gz | 5.8 GB | ||
| EGAF00002044301 | fastq.gz | 6.2 GB | ||
| EGAF00002044302 | fastq.gz | 6.1 GB | ||
| EGAF00002044303 | fastq.gz | 4.4 GB | ||
| EGAF00002044304 | fastq.gz | 4.5 GB | ||
| EGAF00002044305 | fastq.gz | 6.1 GB | ||
| EGAF00002044306 | fastq.gz | 6.3 GB | ||
| EGAF00002044307 | fastq.gz | 5.5 GB | ||
| EGAF00002044308 | fastq.gz | 5.6 GB | ||
| EGAF00002044309 | fastq.gz | 6.0 GB | ||
| EGAF00002044310 | fastq.gz | 6.1 GB | ||
| EGAF00002044311 | fastq.gz | 5.9 GB | ||
| EGAF00002044312 | fastq.gz | 6.0 GB | ||
| EGAF00002044313 | fastq.gz | 4.3 GB | ||
| EGAF00002044314 | fastq.gz | 4.4 GB | ||
| EGAF00002044315 | fastq.gz | 6.0 GB | ||
| EGAF00002044316 | fastq.gz | 6.1 GB | ||
| EGAF00002044317 | fastq.gz | 4.4 GB | ||
| EGAF00002044318 | fastq.gz | 4.5 GB | ||
| EGAF00002044319 | fastq.gz | 4.8 GB | ||
| EGAF00002044320 | fastq.gz | 4.8 GB | ||
| EGAF00002044321 | fastq.gz | 4.3 GB | ||
| EGAF00002044322 | fastq.gz | 4.3 GB | ||
| EGAF00002044323 | fastq.gz | 5.0 GB | ||
| EGAF00002044324 | fastq.gz | 4.9 GB | ||
| EGAF00002044325 | fastq.gz | 7.3 GB | ||
| EGAF00002044326 | fastq.gz | 7.3 GB | ||
| EGAF00002044327 | fastq.gz | 5.5 GB | ||
| EGAF00002044328 | fastq.gz | 5.7 GB | ||
| EGAF00002044329 | fastq.gz | 6.0 GB | ||
| EGAF00002044330 | fastq.gz | 6.1 GB | ||
| EGAF00002044331 | fastq.gz | 4.8 GB | ||
| EGAF00002044332 | fastq.gz | 4.7 GB | ||
| EGAF00002044333 | fastq.gz | 4.1 GB | ||
| EGAF00002044334 | fastq.gz | 4.1 GB | ||
| EGAF00002044335 | fastq.gz | 4.6 GB | ||
| EGAF00002044336 | fastq.gz | 4.6 GB | ||
| EGAF00002044337 | fastq.gz | 6.4 GB | ||
| EGAF00002044338 | fastq.gz | 6.2 GB | ||
| EGAF00002044339 | fastq.gz | 4.3 GB | ||
| EGAF00002044340 | fastq.gz | 4.4 GB | ||
| EGAF00002044341 | fastq.gz | 4.2 GB | ||
| EGAF00002044342 | fastq.gz | 4.1 GB | ||
| EGAF00002044343 | fastq.gz | 4.7 GB | ||
| EGAF00002044344 | fastq.gz | 4.7 GB | ||
| EGAF00002044345 | fastq.gz | 4.2 GB | ||
| EGAF00002044346 | fastq.gz | 4.3 GB | ||
| EGAF00002044347 | fastq.gz | 4.9 GB | ||
| EGAF00002044348 | fastq.gz | 4.8 GB | ||
| EGAF00002044349 | fastq.gz | 4.2 GB | ||
| EGAF00002044350 | fastq.gz | 4.1 GB | ||
| EGAF00002044351 | fastq.gz | 4.3 GB | ||
| EGAF00002044352 | fastq.gz | 4.3 GB | ||
| EGAF00002044353 | fastq.gz | 4.2 GB | ||
| EGAF00002044354 | fastq.gz | 4.2 GB | ||
| EGAF00002044355 | fastq.gz | 4.1 GB | ||
| EGAF00002044356 | fastq.gz | 4.2 GB | ||
| EGAF00002044357 | fastq.gz | 4.5 GB | ||
| EGAF00002044358 | fastq.gz | 4.6 GB | ||
| EGAF00002044359 | fastq.gz | 4.9 GB | ||
| EGAF00002044360 | fastq.gz | 5.1 GB | ||
| EGAF00002044361 | fastq.gz | 6.3 GB | ||
| EGAF00002044362 | fastq.gz | 6.2 GB | ||
| EGAF00002044363 | fastq.gz | 4.8 GB | ||
| EGAF00002044364 | fastq.gz | 4.7 GB | ||
| EGAF00002044365 | fastq.gz | 4.7 GB | ||
| EGAF00002044366 | fastq.gz | 4.6 GB | ||
| EGAF00002044367 | fastq.gz | 4.5 GB | ||
| EGAF00002044368 | fastq.gz | 4.6 GB | ||
| EGAF00002044369 | fastq.gz | 4.5 GB | ||
| EGAF00002044370 | fastq.gz | 4.4 GB | ||
| EGAF00002044371 | fastq.gz | 4.6 GB | ||
| EGAF00002044372 | fastq.gz | 4.4 GB | ||
| EGAF00002044373 | fastq.gz | 4.7 GB | ||
| EGAF00002044374 | fastq.gz | 4.8 GB | ||
| EGAF00002044375 | fastq.gz | 4.9 GB | ||
| EGAF00002044376 | fastq.gz | 4.9 GB | ||
| EGAF00002044377 | fastq.gz | 4.7 GB | ||
| EGAF00002044378 | fastq.gz | 4.7 GB | ||
| EGAF00002044379 | fastq.gz | 6.6 GB | ||
| EGAF00002044380 | fastq.gz | 6.5 GB | ||
| EGAF00002044381 | fastq.gz | 6.0 GB | ||
| EGAF00002044382 | fastq.gz | 6.2 GB | ||
| EGAF00002044383 | fastq.gz | 4.5 GB | ||
| EGAF00002044384 | fastq.gz | 4.5 GB | ||
| EGAF00002044385 | fastq.gz | 4.6 GB | ||
| EGAF00002044386 | fastq.gz | 4.6 GB | ||
| EGAF00002044387 | fastq.gz | 5.3 GB | ||
| EGAF00002044388 | fastq.gz | 5.4 GB | ||
| EGAF00002044389 | fastq.gz | 5.1 GB | ||
| EGAF00002044390 | fastq.gz | 5.0 GB | ||
| EGAF00002044391 | fastq.gz | 4.7 GB | ||
| EGAF00002044392 | fastq.gz | 4.7 GB | ||
| EGAF00002044393 | fastq.gz | 4.6 GB | ||
| EGAF00002044394 | fastq.gz | 4.7 GB | ||
| EGAF00002044395 | fastq.gz | 5.6 GB | ||
| EGAF00002044396 | fastq.gz | 5.5 GB | ||
| EGAF00002044397 | fastq.gz | 5.4 GB | ||
| EGAF00002044398 | fastq.gz | 5.4 GB | ||
| EGAF00002044399 | fastq.gz | 4.6 GB | ||
| EGAF00002044400 | fastq.gz | 4.5 GB | ||
| EGAF00002044401 | fastq.gz | 5.0 GB | ||
| EGAF00002044402 | fastq.gz | 5.0 GB | ||
| EGAF00002044403 | fastq.gz | 4.7 GB | ||
| EGAF00002044404 | fastq.gz | 4.8 GB | ||
| EGAF00002044405 | fastq.gz | 4.8 GB | ||
| EGAF00002044406 | fastq.gz | 4.9 GB | ||
| EGAF00002044407 | fastq.gz | 4.4 GB | ||
| EGAF00002044408 | fastq.gz | 4.7 GB | ||
| EGAF00002044409 | fastq.gz | 4.6 GB | ||
| EGAF00002044410 | fastq.gz | 4.6 GB | ||
| EGAF00002044411 | fastq.gz | 5.0 GB | ||
| EGAF00002044412 | fastq.gz | 4.9 GB | ||
| EGAF00002044413 | fastq.gz | 5.1 GB | ||
| EGAF00002044414 | fastq.gz | 5.0 GB | ||
| EGAF00002044415 | fastq.gz | 5.8 GB | ||
| EGAF00002044416 | fastq.gz | 6.0 GB | ||
| EGAF00002044417 | fastq.gz | 5.6 GB | ||
| EGAF00002044418 | fastq.gz | 5.7 GB | ||
| EGAF00002044419 | fastq.gz | 5.9 GB | ||
| EGAF00002044420 | fastq.gz | 5.9 GB | ||
| EGAF00002044421 | fastq.gz | 5.5 GB | ||
| EGAF00002044422 | fastq.gz | 5.6 GB | ||
| EGAF00002044423 | fastq.gz | 5.3 GB | ||
| EGAF00002044424 | fastq.gz | 5.3 GB | ||
| EGAF00002044425 | fastq.gz | 5.6 GB | ||
| EGAF00002044426 | fastq.gz | 5.7 GB | ||
| EGAF00002044427 | fastq.gz | 7.7 GB | ||
| EGAF00002044428 | fastq.gz | 7.8 GB | ||
| EGAF00002044429 | fastq.gz | 5.0 GB | ||
| EGAF00002044430 | fastq.gz | 5.1 GB | ||
| EGAF00002044431 | fastq.gz | 6.0 GB | ||
| EGAF00002044432 | fastq.gz | 6.1 GB | ||
| EGAF00002044433 | fastq.gz | 6.0 GB | ||
| EGAF00002044434 | fastq.gz | 6.2 GB | ||
| EGAF00002044435 | fastq.gz | 6.5 GB | ||
| EGAF00002044436 | fastq.gz | 6.5 GB | ||
| EGAF00002044437 | fastq.gz | 6.5 GB | ||
| EGAF00002044438 | fastq.gz | 6.7 GB | ||
| EGAF00002044439 | fastq.gz | 5.3 GB | ||
| EGAF00002044440 | fastq.gz | 5.5 GB | ||
| EGAF00002044441 | fastq.gz | 4.9 GB | ||
| EGAF00002044442 | fastq.gz | 5.0 GB | ||
| EGAF00002044443 | fastq.gz | 5.6 GB | ||
| EGAF00002044444 | fastq.gz | 5.7 GB | ||
| EGAF00002044445 | fastq.gz | 5.8 GB | ||
| EGAF00002044446 | fastq.gz | 6.0 GB | ||
| 200 Files (1.0 TB) | ||||