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Prognostic relevance of microenvironmental factors CD163 and CD8 combined with EZH2 and chromosome 18 gain in a validation cohort of follicular lymphoma patients of the Lunenburg Lymphoma Biomarker Consortium

Background: In follicular lymphoma (FL), studies addressing the prognostic value of microenvironment-related immunohistochemical (IHC) markers and tumor cell-related genetic markers have yielded conflicting results, precluding implementation in practice. Therefore, the Lunenburg Lymphoma Biomarker Consortium (LLBC) performed a validation study for published markers. Methods: To maximize sensitivity, an end-of-spectrum design was applied for 122 uniformly immunochemotherapy-treated FL patients retrieved from international trials and registries; early failure (EF): progression or lymphoma-related death <2 years versus long remission: response duration of >5 years. IHC staining for T-cells and macrophages was performed on tissue microarrays from initial biopsy and scored with a validated computer-assisted protocol. Shallow whole-genome and deep targeted sequencing was performed on the same samples. Results: 96/122 cases with complete molecular and immunohistochemical data were included in the analysis. EZH2 wild-type (p=0.006), gain of chromosome 18 (p=0.002), low percentages of CD8+ cells (p=0.011) and CD163+ areas (p=0.038) were associated with EF. No significant differences in other markers were observed, thereby refuting previous claims on their prognostic significance. Conclusion: Using an optimized study design, this LLBC study validates wild-type EZH2 status, gain of chromosome 18, low percentages of CD8+ cells and CD163+ area as predictors of EF to immunochemotherapy in FL.

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Data originating from patient samples

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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.

Study ID Study Title Study Type
Other

This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.

ID File Type Size Located in
EGAF00001386226 fastq.gz 501.4 MB
EGAF00001386227 fastq.gz 429.2 MB
EGAF00001386228 fastq.gz 594.4 MB
EGAF00001386229 fastq.gz 231.7 MB
EGAF00001386230 fastq.gz 254.1 MB
EGAF00001386231 fastq.gz 474.9 MB
EGAF00001386232 fastq.gz 524.9 MB
EGAF00001386233 fastq.gz 800.4 MB
EGAF00001386234 fastq.gz 295.1 MB
EGAF00001386235 fastq.gz 445.6 MB
EGAF00001386236 fastq.gz 635.7 MB
EGAF00001386237 fastq.gz 535.8 MB
EGAF00001386238 fastq.gz 274.8 MB
EGAF00001386239 fastq.gz 318.8 MB
EGAF00001386240 fastq.gz 676.7 MB
EGAF00001386241 fastq.gz 580.9 MB
EGAF00001386242 fastq.gz 540.3 MB
EGAF00001386243 fastq.gz 527.9 MB
EGAF00001386244 fastq.gz 476.0 MB
EGAF00001386245 fastq.gz 590.2 MB
EGAF00001386246 fastq.gz 869.1 MB
EGAF00001386247 fastq.gz 440.9 MB
EGAF00001386248 fastq.gz 462.4 MB
EGAF00001386249 fastq.gz 642.7 MB
EGAF00001386250 fastq.gz 535.7 MB
EGAF00001386251 fastq.gz 1.7 GB
EGAF00001386252 fastq.gz 408.7 MB
EGAF00001386253 fastq.gz 429.3 MB
EGAF00001386254 fastq.gz 605.4 MB
EGAF00001386255 fastq.gz 390.6 MB
EGAF00001386256 fastq.gz 638.3 MB
EGAF00001386257 fastq.gz 424.6 MB
EGAF00001386258 fastq.gz 530.3 MB
EGAF00001386259 fastq.gz 284.6 MB
EGAF00001386260 fastq.gz 232.1 MB
EGAF00001386261 fastq.gz 525.3 MB
EGAF00001386262 fastq.gz 540.6 MB
EGAF00001386263 fastq.gz 743.1 MB
EGAF00001386264 fastq.gz 413.1 MB
EGAF00001386265 fastq.gz 558.8 MB
EGAF00001386266 fastq.gz 531.7 MB
EGAF00001386267 fastq.gz 577.7 MB
EGAF00001386268 fastq.gz 604.6 MB
EGAF00001386269 fastq.gz 650.4 MB
EGAF00001386270 fastq.gz 381.3 MB
EGAF00001386271 fastq.gz 367.3 MB
EGAF00001386272 fastq.gz 456.5 MB
EGAF00001386273 fastq.gz 384.9 MB
EGAF00001386274 fastq.gz 564.6 MB
EGAF00001386275 fastq.gz 375.4 MB
EGAF00001386276 fastq.gz 400.4 MB
EGAF00001386277 fastq.gz 592.8 MB
EGAF00001386278 fastq.gz 628.1 MB
EGAF00001386279 fastq.gz 650.4 MB
EGAF00001386280 fastq.gz 179.2 MB
EGAF00001386281 fastq.gz 228.8 MB
EGAF00001386282 fastq.gz 186.1 MB
EGAF00001386283 fastq.gz 217.4 MB
EGAF00001386284 fastq.gz 540.3 MB
EGAF00001386285 fastq.gz 453.6 MB
EGAF00001386286 fastq.gz 366.5 MB
EGAF00001386287 fastq.gz 447.4 MB
EGAF00001386288 fastq.gz 765.0 MB
EGAF00001386289 fastq.gz 202.9 MB
EGAF00001386290 fastq.gz 950.5 MB
EGAF00001386291 fastq.gz 227.2 MB
EGAF00001386292 fastq.gz 239.2 MB
EGAF00001386293 fastq.gz 1.8 GB
EGAF00001386294 fastq.gz 4.2 GB
EGAF00001386295 fastq.gz 486.5 MB
EGAF00001386296 fastq.gz 258.6 MB
EGAF00001386297 fastq.gz 273.4 MB
EGAF00001386298 fastq.gz 883.9 MB
EGAF00001386299 fastq.gz 557.3 MB
EGAF00001386300 fastq.gz 347.7 MB
EGAF00001386301 fastq.gz 463.5 MB
EGAF00001386302 fastq.gz 1.4 GB
EGAF00001386303 fastq.gz 1.2 GB
EGAF00001386304 fastq.gz 1.1 GB
EGAF00001386305 fastq.gz 1.4 GB
EGAF00001386306 fastq.gz 1.3 GB
EGAF00001386307 fastq.gz 1.3 GB
EGAF00001386308 fastq.gz 1.2 GB
EGAF00001386309 fastq.gz 1.2 GB
EGAF00001386310 fastq.gz 1.2 GB
EGAF00001386311 fastq.gz 1.5 GB
EGAF00001386312 fastq.gz 1.4 GB
EGAF00001386313 fastq.gz 1.5 GB
EGAF00001386314 fastq.gz 1.5 GB
EGAF00001386315 fastq.gz 1.5 GB
EGAF00001386316 fastq.gz 1.4 GB
EGAF00001386317 fastq.gz 1.1 GB
EGAF00001386318 fastq.gz 994.2 MB
EGAF00001386319 fastq.gz 1.3 GB
EGAF00001386320 fastq.gz 1.3 GB
EGAF00001386321 fastq.gz 896.1 MB
EGAF00001386322 fastq.gz 853.4 MB
EGAF00001386323 fastq.gz 1.5 GB
EGAF00001386324 fastq.gz 1.5 GB
EGAF00001386325 fastq.gz 2.1 GB
EGAF00001386326 fastq.gz 2.0 GB
EGAF00001386327 fastq.gz 574.4 MB
EGAF00001386328 fastq.gz 564.5 MB
EGAF00001386329 fastq.gz 4.0 GB
EGAF00001386330 fastq.gz 4.1 GB
EGAF00001386331 fastq.gz 1.2 GB
EGAF00001386332 fastq.gz 1.1 GB
EGAF00001386333 fastq.gz 1.1 GB
EGAF00001386334 fastq.gz 1.1 GB
EGAF00001386335 fastq.gz 1.7 GB
EGAF00001386336 fastq.gz 1.6 GB
EGAF00001386337 fastq.gz 1.1 GB
EGAF00001386338 fastq.gz 1.1 GB
EGAF00001386339 fastq.gz 1.9 GB
EGAF00001386340 fastq.gz 1.8 GB
EGAF00001386341 fastq.gz 2.2 GB
EGAF00001386342 fastq.gz 2.1 GB
EGAF00001386343 fastq.gz 1.3 GB
EGAF00001386344 fastq.gz 1.3 GB
EGAF00001386345 fastq.gz 1.2 GB
EGAF00001386346 fastq.gz 1.2 GB
EGAF00001386347 fastq.gz 2.4 GB
EGAF00001386348 fastq.gz 2.3 GB
EGAF00001386349 fastq.gz 1.5 GB
EGAF00001386350 fastq.gz 1.4 GB
EGAF00001386351 fastq.gz 2.4 GB
EGAF00001386352 fastq.gz 2.6 GB
EGAF00001386353 fastq.gz 788.2 MB
EGAF00001386354 fastq.gz 751.0 MB
EGAF00001386355 fastq.gz 1.3 GB
EGAF00001386356 fastq.gz 1.3 GB
EGAF00001386357 fastq.gz 1.4 GB
EGAF00001386358 fastq.gz 1.4 GB
EGAF00001386359 fastq.gz 1.2 GB
EGAF00001386360 fastq.gz 1.1 GB
EGAF00001386361 fastq.gz 1.6 GB
EGAF00001386362 fastq.gz 1.5 GB
EGAF00001386363 fastq.gz 251.6 MB
EGAF00001386364 fastq.gz 252.1 MB
EGAF00001386365 fastq.gz 468.5 MB
EGAF00001386366 fastq.gz 270.3 MB
EGAF00001386367 fastq.gz 353.3 MB
EGAF00001386368 fastq.gz 784.5 MB
EGAF00001386369 fastq.gz 1.1 GB
EGAF00001386370 fastq.gz 994.2 MB
EGAF00001386371 fastq.gz 352.7 MB
EGAF00001386372 fastq.gz 614.9 MB
EGAF00001386373 fastq.gz 896.1 MB
EGAF00001386374 fastq.gz 261.4 MB
EGAF00001386375 fastq.gz 371.1 MB
EGAF00001386376 fastq.gz 327.7 MB
EGAF00001386377 fastq.gz 410.0 MB
EGAF00001386378 fastq.gz 1.0 GB
EGAF00001386379 fastq.gz 208.9 MB
EGAF00001386380 fastq.gz 223.8 MB
EGAF00001386381 fastq.gz 235.5 MB
EGAF00001386382 fastq.gz 1.2 GB
EGAF00001386383 fastq.gz 1.2 GB
EGAF00001386384 fastq.gz 1.9 GB
EGAF00001386385 fastq.gz 1.8 GB
EGAF00001386386 fastq.gz 1.6 GB
EGAF00001386387 fastq.gz 1.5 GB
EGAF00001386388 fastq.gz 1.1 GB
EGAF00001386389 fastq.gz 1.1 GB
EGAF00001386390 fastq.gz 1.1 GB
EGAF00001386391 fastq.gz 1.1 GB
EGAF00001386392 fastq.gz 1.6 GB
EGAF00001386393 fastq.gz 1.5 GB
EGAF00001386394 fastq.gz 1.4 GB
EGAF00001386395 fastq.gz 1.3 GB
EGAF00001386396 fastq.gz 2.0 GB
EGAF00001386397 fastq.gz 1.9 GB
EGAF00001386398 fastq.gz 640.1 MB
EGAF00001386399 fastq.gz 642.0 MB
EGAF00001386400 fastq.gz 699.9 MB
EGAF00001386401 fastq.gz 707.1 MB
EGAF00001386402 fastq.gz 899.0 MB
EGAF00001386403 fastq.gz 881.7 MB
EGAF00001386404 fastq.gz 943.8 MB
EGAF00001386405 fastq.gz 881.0 MB
EGAF00001386406 fastq.gz 1.0 GB
EGAF00001386407 fastq.gz 1.0 GB
EGAF00001386408 fastq.gz 1.1 GB
EGAF00001386409 fastq.gz 1.1 GB
EGAF00001386410 fastq.gz 5.2 GB
EGAF00001386411 fastq.gz 5.3 GB
EGAF00001386412 fastq.gz 7.0 GB
EGAF00001386413 fastq.gz 7.0 GB
EGAF00001386414 fastq.gz 735.6 MB
EGAF00001386415 fastq.gz 690.3 MB
EGAF00001386416 fastq.gz 1.5 GB
EGAF00001386417 fastq.gz 1.4 GB
EGAF00001386418 fastq.gz 1.5 GB
EGAF00001386419 fastq.gz 1.4 GB
EGAF00001386420 fastq.gz 2.1 GB
EGAF00001386421 fastq.gz 2.0 GB
EGAF00001386422 fastq.gz 1.6 GB
EGAF00001386423 fastq.gz 1.5 GB
EGAF00001386424 fastq.gz 1.2 GB
EGAF00001386425 fastq.gz 1.2 GB
EGAF00001386426 fastq.gz 1.3 GB
EGAF00001386427 fastq.gz 1.3 GB
EGAF00001386428 fastq.gz 1.8 GB
EGAF00001386429 fastq.gz 1.7 GB
EGAF00001386430 fastq.gz 1.4 GB
EGAF00001386431 fastq.gz 1.3 GB
EGAF00001386432 fastq.gz 1.3 GB
EGAF00001386433 fastq.gz 1.2 GB
EGAF00001386434 fastq.gz 1.3 GB
EGAF00001386435 fastq.gz 1.2 GB
EGAF00001386436 fastq.gz 1.3 GB
EGAF00001386437 fastq.gz 1.2 GB
EGAF00001386438 fastq.gz 1.0 GB
EGAF00001386439 fastq.gz 979.7 MB
EGAF00001386440 fastq.gz 1.3 GB
EGAF00001386441 fastq.gz 1.3 GB
EGAF00001386442 fastq.gz 604.8 MB
EGAF00001386443 fastq.gz 620.3 MB
EGAF00001386444 fastq.gz 608.5 MB
EGAF00001386445 fastq.gz 598.1 MB
EGAF00001386446 fastq.gz 118.5 MB
EGAF00001386447 fastq.gz 114.2 MB
EGAF00001386448 fastq.gz 978.1 MB
EGAF00001386449 fastq.gz 908.5 MB
EGAF00001386450 fastq.gz 328.0 MB
EGAF00001386451 fastq.gz 318.5 MB
EGAF00001386452 fastq.gz 1.2 GB
EGAF00001386453 fastq.gz 1.1 GB
EGAF00001386454 fastq.gz 755.3 MB
EGAF00001386455 fastq.gz 769.5 MB
EGAF00001386456 fastq.gz 1.9 GB
EGAF00001386457 fastq.gz 1.8 GB
EGAF00001386458 fastq.gz 788.2 MB
EGAF00001386459 fastq.gz 801.5 MB
EGAF00001386460 fastq.gz 1.2 GB
EGAF00001386461 fastq.gz 1.2 GB
EGAF00001386462 fastq.gz 1.1 GB
EGAF00001386463 fastq.gz 1.1 GB
EGAF00001386464 fastq.gz 1.4 GB
EGAF00001386465 fastq.gz 1.4 GB
EGAF00001386466 fastq.gz 981.5 MB
EGAF00001386467 fastq.gz 924.1 MB
EGAF00001386468 fastq.gz 1.0 GB
EGAF00001386469 fastq.gz 929.4 MB
EGAF00001386470 fastq.gz 1.3 GB
EGAF00001386471 fastq.gz 1.2 GB
EGAF00001386472 fastq.gz 1.4 GB
EGAF00001386473 fastq.gz 1.3 GB
EGAF00001386474 fastq.gz 1.4 GB
EGAF00001386475 fastq.gz 1.3 GB
EGAF00001386476 fastq.gz 1.5 GB
EGAF00001386477 fastq.gz 1.5 GB
EGAF00001386478 fastq.gz 1.3 GB
EGAF00001386479 fastq.gz 1.3 GB
EGAF00001386480 fastq.gz 739.4 MB
EGAF00001386481 fastq.gz 731.2 MB
EGAF00001386482 fastq.gz 1.3 GB
EGAF00001386483 fastq.gz 1.2 GB
EGAF00001386484 fastq.gz 1.2 GB
EGAF00001386485 fastq.gz 1.2 GB
EGAF00001386486 fastq.gz 2.5 GB
EGAF00001386487 fastq.gz 2.4 GB
EGAF00001386488 fastq.gz 3.8 GB
EGAF00001386489 fastq.gz 3.6 GB
EGAF00001386490 fastq.gz 2.7 GB
EGAF00001386491 fastq.gz 2.5 GB
EGAF00001386492 fastq.gz 1.2 GB
EGAF00001386493 fastq.gz 1.2 GB
EGAF00001386494 fastq.gz 1.8 GB
EGAF00001386495 fastq.gz 1.7 GB
EGAF00001386496 fastq.gz 2.1 GB
EGAF00001386497 fastq.gz 2.1 GB
EGAF00001386498 fastq.gz 288.6 MB
EGAF00001386499 fastq.gz 278.4 MB
EGAF00001386500 fastq.gz 1.3 GB
EGAF00001386501 fastq.gz 1.4 GB
EGAF00001386502 fastq.gz 1.6 GB
EGAF00001386503 fastq.gz 1.6 GB
EGAF00001386504 fastq.gz 1.6 GB
EGAF00001386505 fastq.gz 1.6 GB
EGAF00001386506 fastq.gz 3.4 GB
EGAF00001386507 fastq.gz 3.2 GB
EGAF00001386508 fastq.gz 1.1 GB
EGAF00001386509 fastq.gz 1.0 GB
EGAF00001386510 fastq.gz 1.5 GB
EGAF00001386511 fastq.gz 1.4 GB
EGAF00001386512 fastq.gz 1.5 GB
EGAF00001386513 fastq.gz 1.4 GB
288 Files (335.5 GB)