Identification of drug resistance genes in cancer cell lines by insertional mutagenesis
Our aim is to identify genes involved in resistance to anti-cancer therapies. In order to do this we have taken advantage of a lentiviral vector (LV)-based insertional mutagen to mutagenize cancer cell lines. LV-transduced cell lines were then treated with anti-cancer therapies and the emergence of resistant clones scored. DNA from pools of resistant clones was collected, subjected to custom capture by baits designed against the LV sequence, and then sequenced to identify the LV-genomic junction. We hope that the identification of recurrently targeted genes in resistant cell population will allow us to identify genes that mediate drug resistance.
- 28/06/2016
- 71 samples
- DAC: EGAC00001000205
- Technologies: Illumina HiSeq 2500, Illumina MiSeq
Wellcome Trust Sanger Institute Data Sharing Policy
Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| Cancer Genomics |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF00000684442 | cram | 175.8 MB | ||
| EGAF00000684443 | cram | 184.8 MB | ||
| EGAF00000684444 | cram | 166.1 MB | ||
| EGAF00000684445 | cram | 155.9 MB | ||
| EGAF00000684446 | cram | 175.8 MB | ||
| EGAF00000684447 | cram | 166.1 MB | ||
| EGAF00000684448 | cram | 84.8 MB | ||
| EGAF00000684449 | cram | 111.9 MB | ||
| EGAF00000684450 | cram | 136.5 MB | ||
| EGAF00000684451 | cram | 146.2 MB | ||
| EGAF00000684452 | cram | 150.6 MB | ||
| EGAF00000692044 | cram | 216.2 MB | ||
| EGAF00000692045 | cram | 234.3 MB | ||
| EGAF00000692046 | cram | 234.6 MB | ||
| EGAF00000692047 | cram | 132.1 MB | ||
| EGAF00000692048 | cram | 389.2 MB | ||
| EGAF00000692049 | cram | 257.9 MB | ||
| EGAF00000692050 | cram | 94.1 MB | ||
| EGAF00000692051 | cram | 111.8 MB | ||
| EGAF00000692052 | cram | 82.5 MB | ||
| EGAF00000692053 | cram | 67.8 MB | ||
| EGAF00000836589 | cram | 1.3 GB | ||
| EGAF00000836590 | cram | 1.4 GB | ||
| EGAF00000836591 | cram | 1.2 GB | ||
| EGAF00000836592 | cram | 1.2 GB | ||
| EGAF00000836593 | cram | 1.3 GB | ||
| EGAF00000836594 | cram | 1.2 GB | ||
| EGAF00000836595 | cram | 610.2 MB | ||
| EGAF00000836596 | cram | 809.2 MB | ||
| EGAF00000836597 | cram | 1.0 GB | ||
| EGAF00000836598 | cram | 1.1 GB | ||
| EGAF00000836599 | cram | 1.1 GB | ||
| EGAF00000836600 | cram | 1.3 GB | ||
| EGAF00000836601 | cram | 1.4 GB | ||
| EGAF00000836602 | cram | 1.3 GB | ||
| EGAF00000836603 | cram | 1.2 GB | ||
| EGAF00000836604 | cram | 1.4 GB | ||
| EGAF00000836605 | cram | 1.2 GB | ||
| EGAF00000836606 | cram | 638.7 MB | ||
| EGAF00000836607 | cram | 842.9 MB | ||
| EGAF00000836608 | cram | 1.1 GB | ||
| EGAF00000836609 | cram | 1.1 GB | ||
| EGAF00000836610 | cram | 1.2 GB | ||
| EGAF00000836611 | cram | 1.6 GB | ||
| EGAF00000836612 | cram | 1.6 GB | ||
| EGAF00000836613 | cram | 1.7 GB | ||
| EGAF00000836614 | cram | 961.1 MB | ||
| EGAF00000836615 | cram | 2.8 GB | ||
| EGAF00000836616 | cram | 1.9 GB | ||
| EGAF00000836617 | cram | 685.4 MB | ||
| EGAF00000836618 | cram | 853.6 MB | ||
| EGAF00000836619 | cram | 618.5 MB | ||
| EGAF00000836620 | cram | 471.4 MB | ||
| EGAF00000836621 | cram | 1.6 GB | ||
| EGAF00000836622 | cram | 1.6 GB | ||
| EGAF00000836623 | cram | 1.7 GB | ||
| EGAF00000836624 | cram | 960.3 MB | ||
| EGAF00000836625 | cram | 2.8 GB | ||
| EGAF00000836626 | cram | 1.9 GB | ||
| EGAF00000836627 | cram | 685.0 MB | ||
| EGAF00000836628 | cram | 852.9 MB | ||
| EGAF00000836629 | cram | 617.8 MB | ||
| EGAF00000836630 | cram | 471.6 MB | ||
| EGAF00000836631 | cram | 201.8 MB | ||
| EGAF00000836632 | cram | 234.5 MB | ||
| EGAF00000836633 | cram | 162.5 MB | ||
| EGAF00000836634 | cram | 184.6 MB | ||
| EGAF00000836635 | cram | 102.9 MB | ||
| EGAF00000836636 | cram | 144.5 MB | ||
| EGAF00000836637 | cram | 102.5 MB | ||
| EGAF00000836638 | cram | 151.2 MB | ||
| EGAF00000836639 | cram | 184.6 MB | ||
| EGAF00000836640 | cram | 136.5 MB | ||
| EGAF00000836641 | cram | 274.5 MB | ||
| EGAF00000836642 | cram | 259.7 MB | ||
| EGAF00000836643 | cram | 193.5 MB | ||
| EGAF00000836644 | cram | 221.8 MB | ||
| EGAF00000836645 | cram | 80.3 MB | ||
| EGAF00000836646 | cram | 102.9 MB | ||
| EGAF00000836647 | cram | 80.2 MB | ||
| EGAF00000836648 | cram | 186.5 MB | ||
| EGAF00000836649 | cram | 219.7 MB | ||
| EGAF00000836650 | cram | 191.2 MB | ||
| EGAF00000836651 | cram | 238.3 MB | ||
| EGAF00000836652 | cram | 278.3 MB | ||
| EGAF00000836653 | cram | 171.9 MB | ||
| EGAF00000836654 | cram | 195.6 MB | ||
| EGAF00000836655 | cram | 78.9 MB | ||
| EGAF00000836656 | cram | 106.2 MB | ||
| EGAF00000836657 | cram | 81.3 MB | ||
| EGAF00000836658 | cram | 158.9 MB | ||
| EGAF00000836659 | cram | 187.9 MB | ||
| EGAF00000836660 | cram | 158.8 MB | ||
| EGAF00000836661 | cram | 205.3 MB | ||
| EGAF00000836662 | cram | 208.3 MB | ||
| EGAF00000836663 | cram | 171.2 MB | ||
| EGAF00000836664 | cram | 201.2 MB | ||
| EGAF00000836665 | cram | 122.6 MB | ||
| EGAF00000836666 | cram | 161.2 MB | ||
| EGAF00000836667 | cram | 119.9 MB | ||
| EGAF00000836668 | cram | 168.8 MB | ||
| EGAF00000836669 | cram | 207.9 MB | ||
| EGAF00000836670 | cram | 149.7 MB | ||
| EGAF00000836671 | cram | 134.2 MB | ||
| EGAF00000836672 | cram | 134.6 MB | ||
| EGAF00000836673 | cram | 119.9 MB | ||
| EGAF00000836674 | cram | 135.3 MB | ||
| EGAF00000836675 | cram | 85.1 MB | ||
| EGAF00000836676 | cram | 111.3 MB | ||
| EGAF00000836677 | cram | 81.6 MB | ||
| EGAF00000836678 | cram | 117.1 MB | ||
| EGAF00000836679 | cram | 140.2 MB | ||
| EGAF00000836680 | cram | 105.0 MB | ||
| 113 Files (62.4 GB) | ||||