DNA repair in BLM deficient hiPSCs
We are aiming to investigate repair of a double strand break (DSB) within the genome in the presence and absence of the BLOOM protein. Zinc Finger Nucleases introduce DSBs at specified loci within the genome. Using sequencing we will assess the size of the deletion following repair. Protocol 1. Transfect normal and BLOOM deficient human iPS cells with ZFNs, using AMXA 2. Harvest cells after 5 days 3. Perform column extraction of DNA 4. PCR-amplify the ZFN region 5. Sequence and analyse repair of the DSB This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
- 27/05/2015
- 6 samples
- DAC: EGAC00001000332
- Technology: Illumina MiSeq
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Studies are experimental investigations of a particular phenomenon, e.g., case-control studies on a particular trait or cancer research projects reporting matching cancer normal genomes from patients.
| Study ID | Study Title | Study Type |
|---|---|---|
| Other |
This table displays only public information pertaining to the files in the dataset. If you wish to access this dataset, please submit a request. If you already have access to these data files, please consult the download documentation.
| ID | File Type | Size | Located in | |
|---|---|---|---|---|
| EGAF00000512764 | bam | 143.5 MB | ||
| EGAF00000512765 | bam | 135.3 MB | ||
| EGAF00000512766 | bam | 162.4 MB | ||
| EGAF00000512767 | bam | 182.6 MB | ||
| EGAF00000512768 | bam | 145.5 MB | ||
| EGAF00000512769 | bam | 127.9 MB | ||
| 6 Files (897.1 MB) | ||||